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Ischemic preconditioning attenuates morphological and biochemical changes in hepatic ischemia/reperfusion in rats.
Pathobiology. 2010; 77(3):136-46.P

Abstract

OBJECTIVE

Ischemic preconditioning (IPC) has been gradually introduced into clinical liver surgery and transplantation in recent years. However, the protective effects of IPC on hepatic warm ischemia/reperfusion (I/R) injury and the potential mechanisms involved are not fully understood. We aimed to evaluate whether the reduction of apoptotic sinusoidal endothelial cells (SECs), induced by IPC, contributes to its protective effect.

METHODS

Male Wistar rats were randomized into three experimental groups: the continuous clamping group underwent 60 min of 70% hepatic ischemia; the IPC group received 10 min ischemia followed by 10 min reperfusion prior to ischemia, and the sham control (sham) underwent a sham operation without ischemia. Hepatocyte and SEC apoptosis, liver necrotic areas and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid, tumor necrosis factor, myeloperoxidase (MPO) and malondialdehyde were determined. Expression of cysteine-aspartic acid protease-3 (caspase-3) in hepatocytes and SECs was also investigated. Furthermore, the hepatic leukocyte infiltration was assessed by intravital fluorescence microscopy.

RESULTS

IPC exhibited a significant alleviation of their postischemic liver function. Serum AST, ALT and tissue MPO were significantly decreased by IPC, and the degree of hepatocyte and SEC apoptosis was significantly inhibited, as shown by the decreased numbers of adherent leukocytes.

CONCLUSIONS

IPC attenuates hepatic I/R injury by the reduction of leukocyte infiltration, the reduction hepatic enzymatic leakage and the depression of apoptotic cells. SECs are more sensitive to apoptosis induced by warm I/R injury compared to hepatocytes.

Authors+Show Affiliations

Key Laboratory of Combined Multiorgan Transplantation, Ministry of Public Health, Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20516729

Citation

Jin, Li-Ming, et al. "Ischemic Preconditioning Attenuates Morphological and Biochemical Changes in Hepatic Ischemia/reperfusion in Rats." Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology, vol. 77, no. 3, 2010, pp. 136-46.
Jin LM, Liu YX, Zhou L, et al. Ischemic preconditioning attenuates morphological and biochemical changes in hepatic ischemia/reperfusion in rats. Pathobiology. 2010;77(3):136-46.
Jin, L. M., Liu, Y. X., Zhou, L., Xie, H. Y., Feng, X. W., Li, H., & Zheng, S. S. (2010). Ischemic preconditioning attenuates morphological and biochemical changes in hepatic ischemia/reperfusion in rats. Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology, 77(3), 136-46. https://doi.org/10.1159/000292647
Jin LM, et al. Ischemic Preconditioning Attenuates Morphological and Biochemical Changes in Hepatic Ischemia/reperfusion in Rats. Pathobiology. 2010;77(3):136-46. PubMed PMID: 20516729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ischemic preconditioning attenuates morphological and biochemical changes in hepatic ischemia/reperfusion in rats. AU - Jin,Li-Ming, AU - Liu,Yuan-Xing, AU - Zhou,Lin, AU - Xie,Hai-Yang, AU - Feng,Xiao-Wen, AU - Li,Hui, AU - Zheng,Shu-Sen, Y1 - 2010/05/28/ PY - 2009/10/02/received PY - 2009/12/03/accepted PY - 2010/6/3/entrez PY - 2010/6/3/pubmed PY - 2010/8/31/medline SP - 136 EP - 46 JF - Pathobiology : journal of immunopathology, molecular and cellular biology JO - Pathobiology VL - 77 IS - 3 N2 - OBJECTIVE: Ischemic preconditioning (IPC) has been gradually introduced into clinical liver surgery and transplantation in recent years. However, the protective effects of IPC on hepatic warm ischemia/reperfusion (I/R) injury and the potential mechanisms involved are not fully understood. We aimed to evaluate whether the reduction of apoptotic sinusoidal endothelial cells (SECs), induced by IPC, contributes to its protective effect. METHODS: Male Wistar rats were randomized into three experimental groups: the continuous clamping group underwent 60 min of 70% hepatic ischemia; the IPC group received 10 min ischemia followed by 10 min reperfusion prior to ischemia, and the sham control (sham) underwent a sham operation without ischemia. Hepatocyte and SEC apoptosis, liver necrotic areas and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid, tumor necrosis factor, myeloperoxidase (MPO) and malondialdehyde were determined. Expression of cysteine-aspartic acid protease-3 (caspase-3) in hepatocytes and SECs was also investigated. Furthermore, the hepatic leukocyte infiltration was assessed by intravital fluorescence microscopy. RESULTS: IPC exhibited a significant alleviation of their postischemic liver function. Serum AST, ALT and tissue MPO were significantly decreased by IPC, and the degree of hepatocyte and SEC apoptosis was significantly inhibited, as shown by the decreased numbers of adherent leukocytes. CONCLUSIONS: IPC attenuates hepatic I/R injury by the reduction of leukocyte infiltration, the reduction hepatic enzymatic leakage and the depression of apoptotic cells. SECs are more sensitive to apoptosis induced by warm I/R injury compared to hepatocytes. SN - 1423-0291 UR - https://www.unboundmedicine.com/medline/citation/20516729/Ischemic_preconditioning_attenuates_morphological_and_biochemical_changes_in_hepatic_ischemia/reperfusion_in_rats_ L2 - https://www.karger.com?DOI=10.1159/000292647 DB - PRIME DP - Unbound Medicine ER -