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Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS).
PLoS One. 2010 May 24; 5(5):e10783.Plos

Abstract

BACKGROUND

Massive die-offs of little brown bats (Myotis lucifugus) have been occurring since 2006 in hibernation sites around Albany, New York, and this problem has spread to other States in the Northeastern United States. White cottony fungal growth is seen on the snouts of affected animals, a prominent sign of White Nose Syndrome (WNS). A previous report described the involvement of the fungus Geomyces destructans in WNS, but an identical fungus was recently isolated in France from a bat that was evidently healthy. The fungus has been recovered sparsely despite plentiful availability of afflicted animals.

METHODOLOGY/PRINCIPAL FINDINGS

We have investigated 100 bat and environmental samples from eight affected sites in 2008. Our findings provide strong evidence for an etiologic role of G. destructans in bat WNS. (i) Direct smears from bat snouts, Periodic Acid Schiff-stained tissue sections from infected tissues, and scanning electron micrographs of bat tissues all showed fungal structures similar to those of G. destructans. (ii) G. destructans DNA was directly amplified from infected bat tissues, (iii) Isolations of G. destructans in cultures from infected bat tissues showed 100% DNA match with the fungus present in positive tissue samples. (iv) RAPD patterns for all G. destructans cultures isolated from two sites were indistinguishable. (v) The fungal isolates showed psychrophilic growth. (vi) We identified in vitro proteolytic activities suggestive of known fungal pathogenic traits in G. destructans.

CONCLUSIONS/SIGNIFICANCE

Further studies are needed to understand whether G. destructans WNS is a symptom or a trigger for bat mass mortality. The availability of well-characterized G. destructans strains should promote an understanding of bat-fungus relationships, and should aid in the screening of biological and chemical control agents.

Authors+Show Affiliations

Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA. vishnu@wadsworth.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20520731

Citation

Chaturvedi, Vishnu, et al. "Morphological and Molecular Characterizations of Psychrophilic Fungus Geomyces Destructans From New York Bats With White Nose Syndrome (WNS)." PloS One, vol. 5, no. 5, 2010, pp. e10783.
Chaturvedi V, Springer DJ, Behr MJ, et al. Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). PLoS ONE. 2010;5(5):e10783.
Chaturvedi, V., Springer, D. J., Behr, M. J., Ramani, R., Li, X., Peck, M. K., Ren, P., Bopp, D. J., Wood, B., Samsonoff, W. A., Butchkoski, C. M., Hicks, A. C., Stone, W. B., Rudd, R. J., & Chaturvedi, S. (2010). Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). PloS One, 5(5), e10783. https://doi.org/10.1371/journal.pone.0010783
Chaturvedi V, et al. Morphological and Molecular Characterizations of Psychrophilic Fungus Geomyces Destructans From New York Bats With White Nose Syndrome (WNS). PLoS ONE. 2010 May 24;5(5):e10783. PubMed PMID: 20520731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). AU - Chaturvedi,Vishnu, AU - Springer,Deborah J, AU - Behr,Melissa J, AU - Ramani,Rama, AU - Li,Xiaojiang, AU - Peck,Marcia K, AU - Ren,Ping, AU - Bopp,Dianna J, AU - Wood,Britta, AU - Samsonoff,William A, AU - Butchkoski,Calvin M, AU - Hicks,Alan C, AU - Stone,Ward B, AU - Rudd,Robert J, AU - Chaturvedi,Sudha, Y1 - 2010/05/24/ PY - 2010/03/14/received PY - 2010/04/29/accepted PY - 2010/6/4/entrez PY - 2010/6/4/pubmed PY - 2010/9/8/medline SP - e10783 EP - e10783 JF - PloS one JO - PLoS ONE VL - 5 IS - 5 N2 - BACKGROUND: Massive die-offs of little brown bats (Myotis lucifugus) have been occurring since 2006 in hibernation sites around Albany, New York, and this problem has spread to other States in the Northeastern United States. White cottony fungal growth is seen on the snouts of affected animals, a prominent sign of White Nose Syndrome (WNS). A previous report described the involvement of the fungus Geomyces destructans in WNS, but an identical fungus was recently isolated in France from a bat that was evidently healthy. The fungus has been recovered sparsely despite plentiful availability of afflicted animals. METHODOLOGY/PRINCIPAL FINDINGS: We have investigated 100 bat and environmental samples from eight affected sites in 2008. Our findings provide strong evidence for an etiologic role of G. destructans in bat WNS. (i) Direct smears from bat snouts, Periodic Acid Schiff-stained tissue sections from infected tissues, and scanning electron micrographs of bat tissues all showed fungal structures similar to those of G. destructans. (ii) G. destructans DNA was directly amplified from infected bat tissues, (iii) Isolations of G. destructans in cultures from infected bat tissues showed 100% DNA match with the fungus present in positive tissue samples. (iv) RAPD patterns for all G. destructans cultures isolated from two sites were indistinguishable. (v) The fungal isolates showed psychrophilic growth. (vi) We identified in vitro proteolytic activities suggestive of known fungal pathogenic traits in G. destructans. CONCLUSIONS/SIGNIFICANCE: Further studies are needed to understand whether G. destructans WNS is a symptom or a trigger for bat mass mortality. The availability of well-characterized G. destructans strains should promote an understanding of bat-fungus relationships, and should aid in the screening of biological and chemical control agents. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/20520731/Morphological_and_molecular_characterizations_of_psychrophilic_fungus_Geomyces_destructans_from_New_York_bats_with_White_Nose_Syndrome__WNS__ DB - PRIME DP - Unbound Medicine ER -