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Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells.
Inflamm Res. 2010 Dec; 59(12):1027-32.IR

Abstract

OBJECTIVE

This study was designed to examine the mutual relationship between 8-isoprostane in exhaled breath condensate (EBC) and superoxide anion generation by bronchoalveolar lavage fluid (BALF) cells in patients with sarcoidosis.

DESIGN

About 29 patients with sarcoidosis, 34 healthy never smokers (control group for EBC) and 15 healthy never smokers (control group for BAL) were examined. EBC was collected directly before bronchoscopy. 8-Isoprostane was measured by ELISA, and superoxide anion by colorimetry.

RESULTS

Exhaled breath condensate 8-isoprostane is increased in sarcoidosis (median, 25-75 percentile): 2.50; 2.50-3.90 versus 6.20; 2.50-16.95 pg/ml, p ≤ 0.05). Spontaneous superoxide anion release from BALF cells was significantly elevated only in patients with a high percentage of lymphocytes in BALF (6.42 ± 1.24 vs. 23.52 ± 4.30 nmol/10(6) cells, p ≤ 0.01). There were no correlations between 8-isoprostane and spontaneous or stimulated superoxide anion release.

CONCLUSIONS

We confirmed higher concentrations of EBC 8-isoprostane in sarcoidosis and higher spontaneous release of superoxide anion from BALF cells in patients with sarcoidosis. The increase of EBC 8-isoprostane is not directly related to superoxide anion released from BALF cells.

Authors+Show Affiliations

Department of Pneumology and Allergy, Medical University of Lodz, Kopcinskiego Str 22, 90-153, Lodz, Poland. piotrow@toya.net.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20521080

Citation

Piotrowski, Wojciech J., et al. "Exhaled 8-isoprostane in Sarcoidosis: Relation to Superoxide Anion Production By Bronchoalveolar Lavage Cells." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 59, no. 12, 2010, pp. 1027-32.
Piotrowski WJ, Kurmanowska Z, Antczak A, et al. Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells. Inflamm Res. 2010;59(12):1027-32.
Piotrowski, W. J., Kurmanowska, Z., Antczak, A., Marczak, J., Ciebiada, M., & Górski, P. (2010). Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 59(12), 1027-32. https://doi.org/10.1007/s00011-010-0222-4
Piotrowski WJ, et al. Exhaled 8-isoprostane in Sarcoidosis: Relation to Superoxide Anion Production By Bronchoalveolar Lavage Cells. Inflamm Res. 2010;59(12):1027-32. PubMed PMID: 20521080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells. AU - Piotrowski,Wojciech J, AU - Kurmanowska,Zofia, AU - Antczak,Adam, AU - Marczak,Jerzy, AU - Ciebiada,Maciej, AU - Górski,Paweł, Y1 - 2010/06/03/ PY - 2010/02/28/received PY - 2010/05/11/accepted PY - 2010/05/09/revised PY - 2010/6/4/entrez PY - 2010/6/4/pubmed PY - 2011/3/1/medline SP - 1027 EP - 32 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm. Res. VL - 59 IS - 12 N2 - OBJECTIVE: This study was designed to examine the mutual relationship between 8-isoprostane in exhaled breath condensate (EBC) and superoxide anion generation by bronchoalveolar lavage fluid (BALF) cells in patients with sarcoidosis. DESIGN: About 29 patients with sarcoidosis, 34 healthy never smokers (control group for EBC) and 15 healthy never smokers (control group for BAL) were examined. EBC was collected directly before bronchoscopy. 8-Isoprostane was measured by ELISA, and superoxide anion by colorimetry. RESULTS: Exhaled breath condensate 8-isoprostane is increased in sarcoidosis (median, 25-75 percentile): 2.50; 2.50-3.90 versus 6.20; 2.50-16.95 pg/ml, p ≤ 0.05). Spontaneous superoxide anion release from BALF cells was significantly elevated only in patients with a high percentage of lymphocytes in BALF (6.42 ± 1.24 vs. 23.52 ± 4.30 nmol/10(6) cells, p ≤ 0.01). There were no correlations between 8-isoprostane and spontaneous or stimulated superoxide anion release. CONCLUSIONS: We confirmed higher concentrations of EBC 8-isoprostane in sarcoidosis and higher spontaneous release of superoxide anion from BALF cells in patients with sarcoidosis. The increase of EBC 8-isoprostane is not directly related to superoxide anion released from BALF cells. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/20521080/Exhaled_8_isoprostane_in_sarcoidosis:_relation_to_superoxide_anion_production_by_bronchoalveolar_lavage_cells_ L2 - https://dx.doi.org/10.1007/s00011-010-0222-4 DB - PRIME DP - Unbound Medicine ER -