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Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines.
Clin Exp Immunol. 2010 Sep; 161(3):584-90.CE

Abstract

Immunosuppression therapy following lung transplant fails to prevent chronic rejection/bronchiolitis obliterans syndrome, which we have shown is associated with lack of suppression of peripheral blood T cell granzyme B, interferon (IFN)-γ and tumour necrosis factor (TNF)-α. We hypothesized that these proinflammatory mediators may increase with time post-transplant in otherwise stable patients before clinical signs of declining lung function, and patients experiencing declining lung function would show a further increase in these mediators. Intracellular cytokine profiles and granzyme B were investigated in T cells in whole blood and airways from lung transplant patients using flow cytometry. There was a significant negative correlation between forced expiratory volume in 1 s (FEV(1)), drug dose and time post-transplant. A significant correlation between increased granzyme B, IFN-γ, interleukin (IL)-2 and TNF-α and time post-transplant was noted in peripheral blood T cells but not lung T cells from stable patients. Patients with similar drug dose but experiencing declining FEV(1) showed a further increase in peripheral blood T cell IFN-γ, IL-2 and TNF-α. Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines. Declining FEV(1) is associated with a further increase in these proinflammatory mediators. Drugs that reduce these inflammatory mediators effectively may reduce the incidence of chronic graft rejection.

Authors+Show Affiliations

Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, SA, Australia. greg.hodge@health.sa.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20528884

Citation

Hodge, G, et al. "Time Post-lung Transplant Correlates With Increasing Peripheral Blood T Cell Granzyme B and Proinflammatory Cytokines." Clinical and Experimental Immunology, vol. 161, no. 3, 2010, pp. 584-90.
Hodge G, Hodge S, Li-Liew C, et al. Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines. Clin Exp Immunol. 2010;161(3):584-90.
Hodge, G., Hodge, S., Li-Liew, C., Chambers, D., Hopkins, P., Reynolds, P. N., & Holmes, M. (2010). Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines. Clinical and Experimental Immunology, 161(3), 584-90. https://doi.org/10.1111/j.1365-2249.2010.04186.x
Hodge G, et al. Time Post-lung Transplant Correlates With Increasing Peripheral Blood T Cell Granzyme B and Proinflammatory Cytokines. Clin Exp Immunol. 2010;161(3):584-90. PubMed PMID: 20528884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines. AU - Hodge,G, AU - Hodge,S, AU - Li-Liew,C, AU - Chambers,D, AU - Hopkins,P, AU - Reynolds,P N, AU - Holmes,M, PY - 2010/6/10/entrez PY - 2010/6/10/pubmed PY - 2011/2/3/medline SP - 584 EP - 90 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 161 IS - 3 N2 - Immunosuppression therapy following lung transplant fails to prevent chronic rejection/bronchiolitis obliterans syndrome, which we have shown is associated with lack of suppression of peripheral blood T cell granzyme B, interferon (IFN)-γ and tumour necrosis factor (TNF)-α. We hypothesized that these proinflammatory mediators may increase with time post-transplant in otherwise stable patients before clinical signs of declining lung function, and patients experiencing declining lung function would show a further increase in these mediators. Intracellular cytokine profiles and granzyme B were investigated in T cells in whole blood and airways from lung transplant patients using flow cytometry. There was a significant negative correlation between forced expiratory volume in 1 s (FEV(1)), drug dose and time post-transplant. A significant correlation between increased granzyme B, IFN-γ, interleukin (IL)-2 and TNF-α and time post-transplant was noted in peripheral blood T cells but not lung T cells from stable patients. Patients with similar drug dose but experiencing declining FEV(1) showed a further increase in peripheral blood T cell IFN-γ, IL-2 and TNF-α. Time post-lung transplant correlates with increasing peripheral blood T cell granzyme B and proinflammatory cytokines. Declining FEV(1) is associated with a further increase in these proinflammatory mediators. Drugs that reduce these inflammatory mediators effectively may reduce the incidence of chronic graft rejection. SN - 1365-2249 UR - https://www.unboundmedicine.com/medline/citation/20528884/Time_post_lung_transplant_correlates_with_increasing_peripheral_blood_T_cell_granzyme_B_and_proinflammatory_cytokines_ L2 - https://doi.org/10.1111/j.1365-2249.2010.04186.x DB - PRIME DP - Unbound Medicine ER -