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Investigation of ractopamine-imprinted polymer for dispersive solid-phase extraction of trace beta-agonists in pig tissues.
J Sep Sci. 2010 Jul; 33(13):2017-25.JS

Abstract

Ractopamine, as an alternative beta-agonist to clenbuterol, is more and more used as leanness-enhancing agent in the swine industry. This work presents a new molecularly imprinted polymer (MIP) using ractopamine as template for dispersive solid-phase extraction of trace ractopamine and the structural related beta-agonists in animal tissues. The binding properties and selectivity of MIP were investigated. High selectivity in polar environment was found, since the extraction capacity of ractopamine with the MIP was 4.5-fold as much as that with the non-imprinted polymer in acetonitrile. Cross-selectivity investigation indicates that the MIP preferentially binds the template and then the structural analogues according to their molecular similarity. Thermodynamic and kinetic investigation was performed to interpret the specific adsorption and molecular recognition of the MIP for ractopamine. Standard free energy, standard enthalpy, and standard entropy were determined. Related information suggested that adsorption of ractopamine onto MIP was an exothermic, spontaneous process. The MIP can be applied as dispersive solid-phase extraction material for enrichment of ractopamine, isoxsuprine, fenoterol and clenbuterol in complex samples before HPLC analysis. The method revealed detection limits of 0.20-0.90 microg/L, recoveries of 83.8-115.2 and 85.2-110.2% for the spiked pig muscle and pig liver, respectively, with the RSD from 2.5 to 8.8%.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hu Y
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, P. R. China. ceshyl@mail.sysu.edu.cn
Liu R
No affiliation info available
Li Y
No affiliation info available
Li G
No affiliation info available

MeSH

Adrenergic beta-AgonistsAdsorptionAnimalsChromatography, High Pressure LiquidKineticsLiverMolecular ImprintingMuscle, SmoothPhenethylaminesPolymersSolid Phase ExtractionSwineThermodynamics

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20533342

Citation

Hu, Yuling, et al. "Investigation of Ractopamine-imprinted Polymer for Dispersive Solid-phase Extraction of Trace Beta-agonists in Pig Tissues." Journal of Separation Science, vol. 33, no. 13, 2010, pp. 2017-25.
Hu Y, Liu R, Li Y, et al. Investigation of ractopamine-imprinted polymer for dispersive solid-phase extraction of trace beta-agonists in pig tissues. J Sep Sci. 2010;33(13):2017-25.
Hu, Y., Liu, R., Li, Y., & Li, G. (2010). Investigation of ractopamine-imprinted polymer for dispersive solid-phase extraction of trace beta-agonists in pig tissues. Journal of Separation Science, 33(13), 2017-25. https://doi.org/10.1002/jssc.201000063
Hu Y, et al. Investigation of Ractopamine-imprinted Polymer for Dispersive Solid-phase Extraction of Trace Beta-agonists in Pig Tissues. J Sep Sci. 2010;33(13):2017-25. PubMed PMID: 20533342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of ractopamine-imprinted polymer for dispersive solid-phase extraction of trace beta-agonists in pig tissues. AU - Hu,Yuling, AU - Liu,Ruijin, AU - Li,Yuanwen, AU - Li,Gongke, PY - 2010/6/10/entrez PY - 2010/6/10/pubmed PY - 2010/9/24/medline SP - 2017 EP - 25 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 13 N2 - Ractopamine, as an alternative beta-agonist to clenbuterol, is more and more used as leanness-enhancing agent in the swine industry. This work presents a new molecularly imprinted polymer (MIP) using ractopamine as template for dispersive solid-phase extraction of trace ractopamine and the structural related beta-agonists in animal tissues. The binding properties and selectivity of MIP were investigated. High selectivity in polar environment was found, since the extraction capacity of ractopamine with the MIP was 4.5-fold as much as that with the non-imprinted polymer in acetonitrile. Cross-selectivity investigation indicates that the MIP preferentially binds the template and then the structural analogues according to their molecular similarity. Thermodynamic and kinetic investigation was performed to interpret the specific adsorption and molecular recognition of the MIP for ractopamine. Standard free energy, standard enthalpy, and standard entropy were determined. Related information suggested that adsorption of ractopamine onto MIP was an exothermic, spontaneous process. The MIP can be applied as dispersive solid-phase extraction material for enrichment of ractopamine, isoxsuprine, fenoterol and clenbuterol in complex samples before HPLC analysis. The method revealed detection limits of 0.20-0.90 microg/L, recoveries of 83.8-115.2 and 85.2-110.2% for the spiked pig muscle and pig liver, respectively, with the RSD from 2.5 to 8.8%. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20533342/Investigation_of_ractopamine_imprinted_polymer_for_dispersive_solid_phase_extraction_of_trace_beta_agonists_in_pig_tissues_ L2 - https://doi.org/10.1002/jssc.201000063 DB - PRIME DP - Unbound Medicine ER -