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Compartmental analyses of 2H5-alpha-linolenic acid and C-U-eicosapentaenoic acid toward synthesis of plasma labeled 22:6n-3 in newborn term infants.
Am J Clin Nutr. 2010 Aug; 92(2):284-93.AJ

Abstract

BACKGROUND

During early postnatal development, the nervous system accretes docosahexaenoic acid (DHA; 22:6n-3), a highly unsaturated n-3 (omega-3) fatty acid (FA) used in the formation of neural cell membranes. DHA, which is present in human breast milk, may also be biosynthesized from n-3 FAs such as 18:3n-3 [alpha-linolenic acid (ALA)] or 20:5n-3 [eicosapentaenoic acid (EPA)]. An important concern is to what extent these precursors can supply DHA to the developing infant.

OBJECTIVE

We analyzed measurements of fractional percentages of plasma (2)H(5)-ALA and (13)C-U-EPA directed toward the synthesis of labeled 22:6n-3 in 11 newborn infants by using compartmental modeling procedures.

DESIGN

One-week-old infants received doses of (2)H(5)-ALA and (13)C-U-EPA ethyl esters enterally. We drew blood from the infants periodically and analyzed the plasma for endogenous and labeled n-3 FAs. From the time-course concentrations of the labeled FAs, we determined rate constant coefficients, fractional synthetic rates, and plasma turnover rates of n-3 FAs.

RESULTS

In infants, approximately 0.04% of the (2)H(5)-ALA dose converted to plasma (2)H(5)-EPA. Plasma (2)H(5)-EPA and (2)H(5)-22:5n-3 [docosapentaenoic acid (DPA)] efficiently converted to (2)H(5)-DPA and (2)H(5)-DHA, respectively. The percentage of plasma (13)C-U-EPA directed toward the synthesis of (13)C-DHA was lower than the percentage of plasma (2)H(5)-EPA that originated from (2)H(5)-ALA.

CONCLUSIONS

Endogenously synthesized EPA was efficiently converted to DHA. In comparison, preformed EPA was less efficiently used for DHA biosynthesis, which suggests a differential metabolism of endogenous EPA compared with exogenous EPA. However, on a per mole basis, preformed EPA was 3.6 times more effective toward DHA synthesis than was ALA. Newborns required an intake of approximately 5 mg preformed DHA. kg(-1) x d(-1) to maintain plasma DHA homeostasis.

Authors+Show Affiliations

Laboratory of Membrane Biochemistry and Biophysics and Laboratory of Metabolic Control, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20534748

Citation

Lin, Yu Hong, et al. "Compartmental Analyses of 2H5-alpha-linolenic Acid and C-U-eicosapentaenoic Acid Toward Synthesis of Plasma Labeled 22:6n-3 in Newborn Term Infants." The American Journal of Clinical Nutrition, vol. 92, no. 2, 2010, pp. 284-93.
Lin YH, Llanos A, Mena P, et al. Compartmental analyses of 2H5-alpha-linolenic acid and C-U-eicosapentaenoic acid toward synthesis of plasma labeled 22:6n-3 in newborn term infants. Am J Clin Nutr. 2010;92(2):284-93.
Lin, Y. H., Llanos, A., Mena, P., Uauy, R., Salem, N., & Pawlosky, R. J. (2010). Compartmental analyses of 2H5-alpha-linolenic acid and C-U-eicosapentaenoic acid toward synthesis of plasma labeled 22:6n-3 in newborn term infants. The American Journal of Clinical Nutrition, 92(2), 284-93. https://doi.org/10.3945/ajcn.2009.28779
Lin YH, et al. Compartmental Analyses of 2H5-alpha-linolenic Acid and C-U-eicosapentaenoic Acid Toward Synthesis of Plasma Labeled 22:6n-3 in Newborn Term Infants. Am J Clin Nutr. 2010;92(2):284-93. PubMed PMID: 20534748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compartmental analyses of 2H5-alpha-linolenic acid and C-U-eicosapentaenoic acid toward synthesis of plasma labeled 22:6n-3 in newborn term infants. AU - Lin,Yu Hong, AU - Llanos,Adolfo, AU - Mena,Patricia, AU - Uauy,Ricardo, AU - Salem,Norman,Jr AU - Pawlosky,Robert J, Y1 - 2010/06/09/ PY - 2010/6/11/entrez PY - 2010/6/11/pubmed PY - 2010/8/13/medline SP - 284 EP - 93 JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 92 IS - 2 N2 - BACKGROUND: During early postnatal development, the nervous system accretes docosahexaenoic acid (DHA; 22:6n-3), a highly unsaturated n-3 (omega-3) fatty acid (FA) used in the formation of neural cell membranes. DHA, which is present in human breast milk, may also be biosynthesized from n-3 FAs such as 18:3n-3 [alpha-linolenic acid (ALA)] or 20:5n-3 [eicosapentaenoic acid (EPA)]. An important concern is to what extent these precursors can supply DHA to the developing infant. OBJECTIVE: We analyzed measurements of fractional percentages of plasma (2)H(5)-ALA and (13)C-U-EPA directed toward the synthesis of labeled 22:6n-3 in 11 newborn infants by using compartmental modeling procedures. DESIGN: One-week-old infants received doses of (2)H(5)-ALA and (13)C-U-EPA ethyl esters enterally. We drew blood from the infants periodically and analyzed the plasma for endogenous and labeled n-3 FAs. From the time-course concentrations of the labeled FAs, we determined rate constant coefficients, fractional synthetic rates, and plasma turnover rates of n-3 FAs. RESULTS: In infants, approximately 0.04% of the (2)H(5)-ALA dose converted to plasma (2)H(5)-EPA. Plasma (2)H(5)-EPA and (2)H(5)-22:5n-3 [docosapentaenoic acid (DPA)] efficiently converted to (2)H(5)-DPA and (2)H(5)-DHA, respectively. The percentage of plasma (13)C-U-EPA directed toward the synthesis of (13)C-DHA was lower than the percentage of plasma (2)H(5)-EPA that originated from (2)H(5)-ALA. CONCLUSIONS: Endogenously synthesized EPA was efficiently converted to DHA. In comparison, preformed EPA was less efficiently used for DHA biosynthesis, which suggests a differential metabolism of endogenous EPA compared with exogenous EPA. However, on a per mole basis, preformed EPA was 3.6 times more effective toward DHA synthesis than was ALA. Newborns required an intake of approximately 5 mg preformed DHA. kg(-1) x d(-1) to maintain plasma DHA homeostasis. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/20534748/Compartmental_analyses_of_2H5_alpha_linolenic_acid_and_C_U_eicosapentaenoic_acid_toward_synthesis_of_plasma_labeled_22:6n_3_in_newborn_term_infants_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.2009.28779 DB - PRIME DP - Unbound Medicine ER -