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Characterization of rapeseed (Brassica napus) oils by bulk C, O, H, and fatty acid C stable isotope analyses.
J Agric Food Chem. 2010 Jul 14; 58(13):8048-55.JA

Abstract

Rapeseed (Brassica napus) oils differing in cultivar, sites of growth, and harvest year were characterized by fatty acid concentrations and carbon, hydrogen, and oxygen stable isotope analyses of bulk oils (delta(13)C(bulk), delta(2)H(bulk), delta(18)O(bulk) values) and individual fatty acids (delta(13)C(FA)). The delta(13)C(bulk), delta(2)H(bulk), and delta(18)O(bulk) values were determined by continuous flow combustion and high-temperature conversion elemental analyzer-isotope ratio mass spectrometry (EA/IRMS, TC-EA/IRMS). The delta(13)C(FA) values were determined using gas chromatography--combustion-isotope ratio mass spectrometry (GC/C/IRMS). For comparison, other C(3) vegetable oils rich in linolenic acid (flax and false flax oils) and rich in linoleic acid (poppy, sunflower, and safflower oils) were submitted to the same chemical and isotopic analyses. The bulk and molecular delta(13)C values were typical for C(3) plants. The delta(13)C value of palmitic acid (delta(13)C(16:0)) and n-3 alpha-linolenic acid (delta(13)C(18:3n-3)) differed (p < 0.001) between rape, flax, and poppy oils. Also within species, significant differences of delta(13)C(FA) were observed (p < 0.01). The hydrogen and oxygen isotope compositions of rape oil differed between cultivars (p < 0.05). Major differences in the individual delta(13)C(FA) values were found. A plant-specific carbon isotope fractionation occurs during the biosynthesis of the fatty acids and particularly during desaturation of C(18) acids in rape and flax. Bulk oil and specific fatty acid stable isotope analysis might be useful in tracing dietary lipids differing in their origin.

Authors+Show Affiliations

Institute of Plant, Animal and Agroecosystem Sciences, ETH Zurich, Universitaetstrasse 2, CH-8092 Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20536244

Citation

Richter, Eva Katharina, et al. "Characterization of Rapeseed (Brassica Napus) Oils By Bulk C, O, H, and Fatty Acid C Stable Isotope Analyses." Journal of Agricultural and Food Chemistry, vol. 58, no. 13, 2010, pp. 8048-55.
Richter EK, Spangenberg JE, Kreuzer M, et al. Characterization of rapeseed (Brassica napus) oils by bulk C, O, H, and fatty acid C stable isotope analyses. J Agric Food Chem. 2010;58(13):8048-55.
Richter, E. K., Spangenberg, J. E., Kreuzer, M., & Leiber, F. (2010). Characterization of rapeseed (Brassica napus) oils by bulk C, O, H, and fatty acid C stable isotope analyses. Journal of Agricultural and Food Chemistry, 58(13), 8048-55. https://doi.org/10.1021/jf101128f
Richter EK, et al. Characterization of Rapeseed (Brassica Napus) Oils By Bulk C, O, H, and Fatty Acid C Stable Isotope Analyses. J Agric Food Chem. 2010 Jul 14;58(13):8048-55. PubMed PMID: 20536244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of rapeseed (Brassica napus) oils by bulk C, O, H, and fatty acid C stable isotope analyses. AU - Richter,Eva Katharina, AU - Spangenberg,Jorge E, AU - Kreuzer,Michael, AU - Leiber,Florian, PY - 2010/6/12/entrez PY - 2010/6/12/pubmed PY - 2010/10/22/medline SP - 8048 EP - 55 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 13 N2 - Rapeseed (Brassica napus) oils differing in cultivar, sites of growth, and harvest year were characterized by fatty acid concentrations and carbon, hydrogen, and oxygen stable isotope analyses of bulk oils (delta(13)C(bulk), delta(2)H(bulk), delta(18)O(bulk) values) and individual fatty acids (delta(13)C(FA)). The delta(13)C(bulk), delta(2)H(bulk), and delta(18)O(bulk) values were determined by continuous flow combustion and high-temperature conversion elemental analyzer-isotope ratio mass spectrometry (EA/IRMS, TC-EA/IRMS). The delta(13)C(FA) values were determined using gas chromatography--combustion-isotope ratio mass spectrometry (GC/C/IRMS). For comparison, other C(3) vegetable oils rich in linolenic acid (flax and false flax oils) and rich in linoleic acid (poppy, sunflower, and safflower oils) were submitted to the same chemical and isotopic analyses. The bulk and molecular delta(13)C values were typical for C(3) plants. The delta(13)C value of palmitic acid (delta(13)C(16:0)) and n-3 alpha-linolenic acid (delta(13)C(18:3n-3)) differed (p < 0.001) between rape, flax, and poppy oils. Also within species, significant differences of delta(13)C(FA) were observed (p < 0.01). The hydrogen and oxygen isotope compositions of rape oil differed between cultivars (p < 0.05). Major differences in the individual delta(13)C(FA) values were found. A plant-specific carbon isotope fractionation occurs during the biosynthesis of the fatty acids and particularly during desaturation of C(18) acids in rape and flax. Bulk oil and specific fatty acid stable isotope analysis might be useful in tracing dietary lipids differing in their origin. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20536244/Characterization_of_rapeseed__Brassica_napus__oils_by_bulk_C_O_H_and_fatty_acid_C_stable_isotope_analyses_ L2 - https://doi.org/10.1021/jf101128f DB - PRIME DP - Unbound Medicine ER -