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Characterization of norovirus-associated traveler's diarrhea.
Clin Infect Dis. 2010 Jul 15; 51(2):123-30.CI

Abstract

BACKGROUND

Traveler's diarrhea is the most common medical complaint of international visitors to developing regions. Previous findings suggested that noroviruses (NoVs) are an underappreciated cause of traveler's diarrhea. METHODS. In the present study, we sought to define the presence of NoVs in 320 acute diarrheic stool samples collected from 299 US students who traveled to Guadalajara, Cuernavaca, or Puerto Vallarta, Mexico, during the period from 2007 through 2008. Conventional and quantitative real-time polymerase chain reaction assays were used to detect and determine NoV loads in stool samples. NoV strains were characterized by purification of viral RNA followed by sequencing of the viral capsid protein 1 gene. Sequences were compared using multiple sequence alignment, and phylogenetic trees were generated to evaluate the evolutionary relatedness of the viral strains associated with cases of traveler's diarrhea.

RESULTS

NoV RNA was detected in 30 (9.4%) of 320 samples. Twelve strains belonged to genogroup I, and 18 strains belonged to genogroup II. NoV prevalence was higher in the winter season than in the summer season (23% vs 7%, respectively; P = .001). The cDNA viral loads of genogroup I viruses were found to be 500-fold higher than those of genogroup II strains. Phylogenetic analysis revealed a diverse population of NoV strains over different locations and years.

CONCLUSIONS

NoV strains are important causes of traveler's diarrhea in Mexico, especially during the wintertime, and US students in Mexico may represent a suitable group for future NoV vaccine efficacy trials.

Authors+Show Affiliations

Department of Molecular Virology and Microbiology and Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20540620

Citation

Ajami, N, et al. "Characterization of Norovirus-associated Traveler's Diarrhea." Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, vol. 51, no. 2, 2010, pp. 123-30.
Ajami N, Koo H, Darkoh C, et al. Characterization of norovirus-associated traveler's diarrhea. Clin Infect Dis. 2010;51(2):123-30.
Ajami, N., Koo, H., Darkoh, C., Atmar, R. L., Okhuysen, P. C., Jiang, Z. D., Flores, J., & Dupont, H. L. (2010). Characterization of norovirus-associated traveler's diarrhea. Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America, 51(2), 123-30. https://doi.org/10.1086/653530
Ajami N, et al. Characterization of Norovirus-associated Traveler's Diarrhea. Clin Infect Dis. 2010 Jul 15;51(2):123-30. PubMed PMID: 20540620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of norovirus-associated traveler's diarrhea. AU - Ajami,N, AU - Koo,H, AU - Darkoh,C, AU - Atmar,R L, AU - Okhuysen,P C, AU - Jiang,Z-D, AU - Flores,J, AU - Dupont,H L, PY - 2010/6/15/entrez PY - 2010/6/15/pubmed PY - 2010/9/21/medline SP - 123 EP - 30 JF - Clinical infectious diseases : an official publication of the Infectious Diseases Society of America JO - Clin Infect Dis VL - 51 IS - 2 N2 - BACKGROUND: Traveler's diarrhea is the most common medical complaint of international visitors to developing regions. Previous findings suggested that noroviruses (NoVs) are an underappreciated cause of traveler's diarrhea. METHODS. In the present study, we sought to define the presence of NoVs in 320 acute diarrheic stool samples collected from 299 US students who traveled to Guadalajara, Cuernavaca, or Puerto Vallarta, Mexico, during the period from 2007 through 2008. Conventional and quantitative real-time polymerase chain reaction assays were used to detect and determine NoV loads in stool samples. NoV strains were characterized by purification of viral RNA followed by sequencing of the viral capsid protein 1 gene. Sequences were compared using multiple sequence alignment, and phylogenetic trees were generated to evaluate the evolutionary relatedness of the viral strains associated with cases of traveler's diarrhea. RESULTS: NoV RNA was detected in 30 (9.4%) of 320 samples. Twelve strains belonged to genogroup I, and 18 strains belonged to genogroup II. NoV prevalence was higher in the winter season than in the summer season (23% vs 7%, respectively; P = .001). The cDNA viral loads of genogroup I viruses were found to be 500-fold higher than those of genogroup II strains. Phylogenetic analysis revealed a diverse population of NoV strains over different locations and years. CONCLUSIONS: NoV strains are important causes of traveler's diarrhea in Mexico, especially during the wintertime, and US students in Mexico may represent a suitable group for future NoV vaccine efficacy trials. SN - 1537-6591 UR - https://www.unboundmedicine.com/medline/citation/20540620/Characterization_of_norovirus_associated_traveler's_diarrhea_ L2 - https://academic.oup.com/cid/article-lookup/doi/10.1086/653530 DB - PRIME DP - Unbound Medicine ER -