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Significance and confounders of peripheral DJ-1 and alpha-synuclein in Parkinson's disease.
Neurosci Lett. 2010 Aug 09; 480(1):78-82.NL

Abstract

DJ-1 and alpha-synuclein are leading biomarkers for Parkinson's disease diagnosis and/or monitoring disease progression. A few recent investigations have determined DJ-1 and alpha-synuclein levels in plasma or serum, a more convenient sample source than cerebrospinal fluid; but the results were variable or even contradictory. Besides limitations in detection technology and limited number of cases in some studies, inadequate control of several important confounders likely has contributed to these inconsistent results. In this study, the relative contribution of each blood component to blood DJ-1 and alpha-synuclein was evaluated, followed by quantification of plasma levels of both markers in a larger cohort of patients/subjects (approximately 300 cases) whose cerebrospinal fluid DJ-1 and alpha-synuclein levels have been determined recently. The results demonstrated that the DJ-1 and alpha-synuclein in blood resided predominantly in red blood cells (>95%), followed by platelets (1-4%), white blood cells and plasma (< or =1%), indicating that variations in hemolysis and/or platelet contamination could have a significant effect on plasma/serum DJ-1 and alpha-synuclein levels. Nonetheless, after adjusting for the age, although there was a trend of decrease in DJ-1 and alpha-synuclein in patients with Parkinson's or Alzheimer's disease compared with healthy controls, no statistical difference was observed in this cohort between any groups, even when the extent of hemolysis and platelet contamination were controlled for. Additionally, no correlation between DJ-1 or alpha-synuclein and Parkinson's disease severity was identified. In conclusion, unlike in cerebrospinal fluid, total DJ-1 or alpha-synuclein in plasma alone is not useful as biomarkers for Parkinson's disease diagnosis or progression/severity.

Authors+Show Affiliations

Department of Pathology, University of Washington School of Medicine, Seattle, WA 98104, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20540987

Citation

Shi, Min, et al. "Significance and Confounders of Peripheral DJ-1 and Alpha-synuclein in Parkinson's Disease." Neuroscience Letters, vol. 480, no. 1, 2010, pp. 78-82.
Shi M, Zabetian CP, Hancock AM, et al. Significance and confounders of peripheral DJ-1 and alpha-synuclein in Parkinson's disease. Neurosci Lett. 2010;480(1):78-82.
Shi, M., Zabetian, C. P., Hancock, A. M., Ginghina, C., Hong, Z., Yearout, D., Chung, K. A., Quinn, J. F., Peskind, E. R., Galasko, D., Jankovic, J., Leverenz, J. B., & Zhang, J. (2010). Significance and confounders of peripheral DJ-1 and alpha-synuclein in Parkinson's disease. Neuroscience Letters, 480(1), 78-82. https://doi.org/10.1016/j.neulet.2010.06.009
Shi M, et al. Significance and Confounders of Peripheral DJ-1 and Alpha-synuclein in Parkinson's Disease. Neurosci Lett. 2010 Aug 9;480(1):78-82. PubMed PMID: 20540987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Significance and confounders of peripheral DJ-1 and alpha-synuclein in Parkinson's disease. AU - Shi,Min, AU - Zabetian,Cyrus P, AU - Hancock,Aneeka M, AU - Ginghina,Carmen, AU - Hong,Zhen, AU - Yearout,Dora, AU - Chung,Kathryn A, AU - Quinn,Joseph F, AU - Peskind,Elaine R, AU - Galasko,Douglas, AU - Jankovic,Joseph, AU - Leverenz,James B, AU - Zhang,Jing, Y1 - 2010/06/09/ PY - 2010/03/30/received PY - 2010/05/30/revised PY - 2010/06/01/accepted PY - 2010/6/15/entrez PY - 2010/6/15/pubmed PY - 2010/9/25/medline SP - 78 EP - 82 JF - Neuroscience letters JO - Neurosci Lett VL - 480 IS - 1 N2 - DJ-1 and alpha-synuclein are leading biomarkers for Parkinson's disease diagnosis and/or monitoring disease progression. A few recent investigations have determined DJ-1 and alpha-synuclein levels in plasma or serum, a more convenient sample source than cerebrospinal fluid; but the results were variable or even contradictory. Besides limitations in detection technology and limited number of cases in some studies, inadequate control of several important confounders likely has contributed to these inconsistent results. In this study, the relative contribution of each blood component to blood DJ-1 and alpha-synuclein was evaluated, followed by quantification of plasma levels of both markers in a larger cohort of patients/subjects (approximately 300 cases) whose cerebrospinal fluid DJ-1 and alpha-synuclein levels have been determined recently. The results demonstrated that the DJ-1 and alpha-synuclein in blood resided predominantly in red blood cells (>95%), followed by platelets (1-4%), white blood cells and plasma (< or =1%), indicating that variations in hemolysis and/or platelet contamination could have a significant effect on plasma/serum DJ-1 and alpha-synuclein levels. Nonetheless, after adjusting for the age, although there was a trend of decrease in DJ-1 and alpha-synuclein in patients with Parkinson's or Alzheimer's disease compared with healthy controls, no statistical difference was observed in this cohort between any groups, even when the extent of hemolysis and platelet contamination were controlled for. Additionally, no correlation between DJ-1 or alpha-synuclein and Parkinson's disease severity was identified. In conclusion, unlike in cerebrospinal fluid, total DJ-1 or alpha-synuclein in plasma alone is not useful as biomarkers for Parkinson's disease diagnosis or progression/severity. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/20540987/Significance_and_confounders_of_peripheral_DJ_1_and_alpha_synuclein_in_Parkinson's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(10)00742-1 DB - PRIME DP - Unbound Medicine ER -