Tags

Type your tag names separated by a space and hit enter

Sulfuretin isolated from heartwood of Rhus verniciflua inhibits LPS-induced inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines expression via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells.
Int Immunopharmacol 2010; 10(8):943-50II

Abstract

It has been reported that Rhusverniciflua exhibits anti-inflammatory, anti-oxidant and anti-cancer activities. However, little is known about biological activity of sulfuretin, a flavonoid isolated from R.verniciflua. In the present study, we investigated the anti-inflammatory effect and the underlying molecular mechanisms of sulfuretin in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Sulfuretin dose-dependently reduced the productions of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) induced by LPS. Consistent with these findings, sulfuretin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-alpha, and IL-1 beta. In addition, sulfuretin attenuated LPS-induced DNA binding and the transcriptional activities of nuclear factor-kappa B (NF-kappaB), which was accompanied by a parallel reduction of degradation and phosphorylation of inhibitory kappa B-alpha (I kappaB-alpha) and consequently by decreased nuclear translocation of p65 subunit of NF-kappaB. Furthermore, pretreatment with sulfuretin significantly inhibited the LPS-stimulated activation of I kappaB kinase beta (IKK beta). Taken together, these results suggest that the anti-inflammatory effect of sulfuretin in LPS-treated RAW 264.7 macrophages is associated with the suppression of NF-kappaB transcriptional activity via the inhibitory regulation of IKKbeta phosphorylation.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20546946

Citation

Shin, Ji-Sun, et al. "Sulfuretin Isolated From Heartwood of Rhus Verniciflua Inhibits LPS-induced Inducible Nitric Oxide Synthase, Cyclooxygenase-2, and Pro-inflammatory Cytokines Expression Via the Down-regulation of NF-kappaB in RAW 264.7 Murine Macrophage Cells." International Immunopharmacology, vol. 10, no. 8, 2010, pp. 943-50.
Shin JS, Park YM, Choi JH, et al. Sulfuretin isolated from heartwood of Rhus verniciflua inhibits LPS-induced inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines expression via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. Int Immunopharmacol. 2010;10(8):943-50.
Shin, J. S., Park, Y. M., Choi, J. H., Park, H. J., Shin, M. C., Lee, Y. S., & Lee, K. T. (2010). Sulfuretin isolated from heartwood of Rhus verniciflua inhibits LPS-induced inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines expression via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. International Immunopharmacology, 10(8), pp. 943-50. doi:10.1016/j.intimp.2010.05.007.
Shin JS, et al. Sulfuretin Isolated From Heartwood of Rhus Verniciflua Inhibits LPS-induced Inducible Nitric Oxide Synthase, Cyclooxygenase-2, and Pro-inflammatory Cytokines Expression Via the Down-regulation of NF-kappaB in RAW 264.7 Murine Macrophage Cells. Int Immunopharmacol. 2010;10(8):943-50. PubMed PMID: 20546946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sulfuretin isolated from heartwood of Rhus verniciflua inhibits LPS-induced inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines expression via the down-regulation of NF-kappaB in RAW 264.7 murine macrophage cells. AU - Shin,Ji-Sun, AU - Park,Young Mi, AU - Choi,Jung-Hye, AU - Park,Hee-Juhn, AU - Shin,Min Cheol, AU - Lee,Yong Sup, AU - Lee,Kyung-Tae, Y1 - 2010/05/28/ PY - 2010/02/11/received PY - 2010/05/08/revised PY - 2010/05/16/accepted PY - 2010/6/16/entrez PY - 2010/6/16/pubmed PY - 2011/3/4/medline SP - 943 EP - 50 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 10 IS - 8 N2 - It has been reported that Rhusverniciflua exhibits anti-inflammatory, anti-oxidant and anti-cancer activities. However, little is known about biological activity of sulfuretin, a flavonoid isolated from R.verniciflua. In the present study, we investigated the anti-inflammatory effect and the underlying molecular mechanisms of sulfuretin in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Sulfuretin dose-dependently reduced the productions of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) induced by LPS. Consistent with these findings, sulfuretin significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-alpha, and IL-1 beta. In addition, sulfuretin attenuated LPS-induced DNA binding and the transcriptional activities of nuclear factor-kappa B (NF-kappaB), which was accompanied by a parallel reduction of degradation and phosphorylation of inhibitory kappa B-alpha (I kappaB-alpha) and consequently by decreased nuclear translocation of p65 subunit of NF-kappaB. Furthermore, pretreatment with sulfuretin significantly inhibited the LPS-stimulated activation of I kappaB kinase beta (IKK beta). Taken together, these results suggest that the anti-inflammatory effect of sulfuretin in LPS-treated RAW 264.7 macrophages is associated with the suppression of NF-kappaB transcriptional activity via the inhibitory regulation of IKKbeta phosphorylation. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/20546946/Sulfuretin_isolated_from_heartwood_of_Rhus_verniciflua_inhibits_LPS_induced_inducible_nitric_oxide_synthase_cyclooxygenase_2_and_pro_inflammatory_cytokines_expression_via_the_down_regulation_of_NF_kappaB_in_RAW_264_7_murine_macrophage_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(10)00166-9 DB - PRIME DP - Unbound Medicine ER -