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Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies.
Environ Toxicol. 2011 Nov; 26(6):623-32.ET

Abstract

The aim of the present study was to measure the early effects of copper (10 and 50 μg L(-1)), cadmium (2, 10, and 50 μg L(-1)) and mixtures of these metals in the freshwater bivalve Corbicula fluminea exposed for 12 h in laboratory. Transcription levels of superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GPx), pi-class glutathione S-transferase (pi-GST), metallothionein (MT) in digestive gland and gills, and response of lysosomal system and neutral lipids in digestive gland were determined after the exposure period. Results showed that lysosomal system, neutral lipids content, and mRNA levels were modified, suggesting their early response against oxidative stress and their important role in cell integrity. The integrated biomarker response was calculated and showed that the effects of the combinations of Cu and Cd on the biomarker responses are additive. MT and pi-GST mRNA expression correspond to the largest ranges of response. As efficient biomarkers should have an early warning capacity, SOD, CAT, Se-GPx, pi-GST, MT transcripts levels, lysosomal system, and neutral lipids could be used as biomarkers of metal contamination in the aquatic environment.

Authors+Show Affiliations

Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes, Université Paul Verlaine-Metz, CNRS UMR 7146, Rue Delestraint, 57070 Metz, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20549629

Citation

Bigot, Aurélie, et al. "Early Defense Responses in the Freshwater Bivalve Corbicula Fluminea Exposed to Copper and Cadmium: Transcriptional and Histochemical Studies." Environmental Toxicology, vol. 26, no. 6, 2011, pp. 623-32.
Bigot A, Minguez L, Giambérini L, et al. Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies. Environ Toxicol. 2011;26(6):623-32.
Bigot, A., Minguez, L., Giambérini, L., & Rodius, F. (2011). Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies. Environmental Toxicology, 26(6), 623-32. https://doi.org/10.1002/tox.20599
Bigot A, et al. Early Defense Responses in the Freshwater Bivalve Corbicula Fluminea Exposed to Copper and Cadmium: Transcriptional and Histochemical Studies. Environ Toxicol. 2011;26(6):623-32. PubMed PMID: 20549629.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early defense responses in the freshwater bivalve Corbicula fluminea exposed to copper and cadmium: Transcriptional and histochemical studies. AU - Bigot,Aurélie, AU - Minguez,Laëtitia, AU - Giambérini,Laure, AU - Rodius,François, Y1 - 2010/04/12/ PY - 2009/03/13/received PY - 2010/02/19/revised PY - 2010/02/20/accepted PY - 2010/6/16/entrez PY - 2010/6/16/pubmed PY - 2012/3/1/medline SP - 623 EP - 32 JF - Environmental toxicology JO - Environ Toxicol VL - 26 IS - 6 N2 - The aim of the present study was to measure the early effects of copper (10 and 50 μg L(-1)), cadmium (2, 10, and 50 μg L(-1)) and mixtures of these metals in the freshwater bivalve Corbicula fluminea exposed for 12 h in laboratory. Transcription levels of superoxide dismutase (SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GPx), pi-class glutathione S-transferase (pi-GST), metallothionein (MT) in digestive gland and gills, and response of lysosomal system and neutral lipids in digestive gland were determined after the exposure period. Results showed that lysosomal system, neutral lipids content, and mRNA levels were modified, suggesting their early response against oxidative stress and their important role in cell integrity. The integrated biomarker response was calculated and showed that the effects of the combinations of Cu and Cd on the biomarker responses are additive. MT and pi-GST mRNA expression correspond to the largest ranges of response. As efficient biomarkers should have an early warning capacity, SOD, CAT, Se-GPx, pi-GST, MT transcripts levels, lysosomal system, and neutral lipids could be used as biomarkers of metal contamination in the aquatic environment. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/20549629/Early_defense_responses_in_the_freshwater_bivalve_Corbicula_fluminea_exposed_to_copper_and_cadmium:_Transcriptional_and_histochemical_studies_ L2 - https://doi.org/10.1002/tox.20599 DB - PRIME DP - Unbound Medicine ER -