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Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography.
J Sep Sci. 2010 Aug; 33(15):2266-71.JS

Abstract

Three hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one-step by both reversed-phase and normal-phase high-speed counter-current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:10:0.2:0.2:20) by reversed-phase high-speed counter-current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (0.2:10:2:1:5) by normal-phase high-speed counter-current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed-phase and normal-phase high-speed counter-current chromatography to separate high-polarity of low-molecular-weight substances.

Authors+Show Affiliations

School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20549664

Citation

Liu, Dan, et al. "Separation and Purification of Hydrolyzable Tannin From Geranium Wilfordii Maxim By Reversed-phase and Normal-phase High-speed Counter-current Chromatography." Journal of Separation Science, vol. 33, no. 15, 2010, pp. 2266-71.
Liu D, Su Z, Wang C, et al. Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography. J Sep Sci. 2010;33(15):2266-71.
Liu, D., Su, Z., Wang, C., Gu, M., & Xing, S. (2010). Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography. Journal of Separation Science, 33(15), 2266-71. https://doi.org/10.1002/jssc.200900599
Liu D, et al. Separation and Purification of Hydrolyzable Tannin From Geranium Wilfordii Maxim By Reversed-phase and Normal-phase High-speed Counter-current Chromatography. J Sep Sci. 2010;33(15):2266-71. PubMed PMID: 20549664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography. AU - Liu,Dan, AU - Su,Zhiguo, AU - Wang,Changhai, AU - Gu,Ming, AU - Xing,Siliang, PY - 2010/6/16/entrez PY - 2010/6/16/pubmed PY - 2010/12/14/medline SP - 2266 EP - 71 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 15 N2 - Three hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one-step by both reversed-phase and normal-phase high-speed counter-current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (1:10:0.2:0.2:20) by reversed-phase high-speed counter-current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n-hexane-ethyl acetate-methanol-acetic acid-water (0.2:10:2:1:5) by normal-phase high-speed counter-current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed-phase and normal-phase high-speed counter-current chromatography to separate high-polarity of low-molecular-weight substances. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/20549664/Separation_and_purification_of_hydrolyzable_tannin_from_Geranium_wilfordii_Maxim_by_reversed_phase_and_normal_phase_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/jssc.200900599 DB - PRIME DP - Unbound Medicine ER -