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Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders.
J Sci Food Agric 2010; 90(9):1473-8JS

Abstract

BACKGROUND

The objective of this study was to validate an improved 4-dimethylaminocinnamaldehyde (DMAC) colorimetric method using a commercially available standard (procyanidin A2), for the standard method for quantification of proanthocyanidins (PACs) in cranberry powders, in order to establish dosage guidelines for the uropathogenic bacterial anti-adhesion effect of cranberry.

RESULTS

Commercially available cranberry samples were obtained (five from U.S. sources and six from European sources) for PAC quantification in five different analytical laboratories. Each laboratory extracted and analyzed the samples using the improved DMAC method. Within-laboratory variation (mean +/- SD) was 4.1 +/- 1.7% RSD (range, 2.3-6.1% RSD) and the between laboratory variability was 16.9 +/- 8.5% RSD (range, 8-32% RSD). For comparative purposes, the cranberry samples were alternatively quantified using weights of extracted PACs (gravimetric). The correlation coefficient between the two methods was 0.989.

CONCLUSION

This improved DMAC method provides a simple, robust and relatively specific spectrophotometric assay for total PACs in cranberry samples using commercially available procyanidin A2 dimer as a standard. DMAC is most useful within a given type of food such as cranberries, but may not be appropriate for comparing concentrations across different food types, particularly in those cases where large differences exist among the relative amounts of each oligomer and polymer.

Authors+Show Affiliations

USDA-ARS, Arkansas Children's Nutrition Center, Little Rock, AR 72202, USA. priorronaldl@uams.edu

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Validation Studies

Language

eng

PubMed ID

20549799

Citation

Prior, Ronald L., et al. "Multi-laboratory Validation of a Standard Method for Quantifying Proanthocyanidins in Cranberry Powders." Journal of the Science of Food and Agriculture, vol. 90, no. 9, 2010, pp. 1473-8.
Prior RL, Fan E, Ji H, et al. Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. J Sci Food Agric. 2010;90(9):1473-8.
Prior, R. L., Fan, E., Ji, H., Howell, A., Nio, C., Payne, M. J., & Reed, J. (2010). Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. Journal of the Science of Food and Agriculture, 90(9), pp. 1473-8. doi:10.1002/jsfa.3966.
Prior RL, et al. Multi-laboratory Validation of a Standard Method for Quantifying Proanthocyanidins in Cranberry Powders. J Sci Food Agric. 2010;90(9):1473-8. PubMed PMID: 20549799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. AU - Prior,Ronald L, AU - Fan,Ellen, AU - Ji,Hongping, AU - Howell,Amy, AU - Nio,Christian, AU - Payne,Mark J, AU - Reed,Jess, PY - 2010/6/16/entrez PY - 2010/6/16/pubmed PY - 2010/10/14/medline SP - 1473 EP - 8 JF - Journal of the science of food and agriculture JO - J. Sci. Food Agric. VL - 90 IS - 9 N2 - BACKGROUND: The objective of this study was to validate an improved 4-dimethylaminocinnamaldehyde (DMAC) colorimetric method using a commercially available standard (procyanidin A2), for the standard method for quantification of proanthocyanidins (PACs) in cranberry powders, in order to establish dosage guidelines for the uropathogenic bacterial anti-adhesion effect of cranberry. RESULTS: Commercially available cranberry samples were obtained (five from U.S. sources and six from European sources) for PAC quantification in five different analytical laboratories. Each laboratory extracted and analyzed the samples using the improved DMAC method. Within-laboratory variation (mean +/- SD) was 4.1 +/- 1.7% RSD (range, 2.3-6.1% RSD) and the between laboratory variability was 16.9 +/- 8.5% RSD (range, 8-32% RSD). For comparative purposes, the cranberry samples were alternatively quantified using weights of extracted PACs (gravimetric). The correlation coefficient between the two methods was 0.989. CONCLUSION: This improved DMAC method provides a simple, robust and relatively specific spectrophotometric assay for total PACs in cranberry samples using commercially available procyanidin A2 dimer as a standard. DMAC is most useful within a given type of food such as cranberries, but may not be appropriate for comparing concentrations across different food types, particularly in those cases where large differences exist among the relative amounts of each oligomer and polymer. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/20549799/Multi_laboratory_validation_of_a_standard_method_for_quantifying_proanthocyanidins_in_cranberry_powders_ L2 - https://doi.org/10.1002/jsfa.3966 DB - PRIME DP - Unbound Medicine ER -