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Fate of Aegilops speltoides-derived, repetitive DNA sequences in diploid Aegilops species, wheat-Aegilops amphiploids and derived chromosome addition lines.
Cytogenet Genome Res. 2010 Jul; 129(1-3):47-54.CG

Abstract

The present study reports the cloning and characterization of an Aegilops speltoides-derived subtelomeric repeat, designated as pSp1B16. Clone pSp1B16 has 98% sequence homology with the previously isolated Ae. speltoides repeat Spelt1. The distribution of pSp1B16 and another Ae. speltoides repeat, pGc1R1, was analyzed in diploid Aegilops species, tetra- and hexaploid wheats, wheat-Aegilops amphiploids and derived chromosome addition lines by fluorescence in situ hybridization (FISH). Clones pSp1B16 and pGc1R1 revealed FISH sites in Ae. speltoides, Ae. sharonensis and Triticum timopheevii, whereas additional pGc1R1 FISH sites were observed in Ae. longissima and Ae. caudata. The pSp1B16 and pGc1R1 FISH patterns of the Aegilops chromosomes in the wheat-Aegilops amphiploids and chromosome addition lines are similar to those present in the Aegilops parent accession. We did not observe any evidence of pSp1B16 and pGc1R1 sequence elimination, which is in contrast to previous studies using similar hybrids and repeats. The presented data suggest that the genomic changes in synthetic amphiploids observed in previous studies might be caused by homoeologous recombination, which was suppressed in the amphiploid analyzed in this study.

Authors+Show Affiliations

Molecular Cytogenetics Laboratory, Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20551615

Citation

Kumar, S, et al. "Fate of Aegilops Speltoides-derived, Repetitive DNA Sequences in Diploid Aegilops Species, wheat-Aegilops Amphiploids and Derived Chromosome Addition Lines." Cytogenetic and Genome Research, vol. 129, no. 1-3, 2010, pp. 47-54.
Kumar S, Friebe B, Gill BS. Fate of Aegilops speltoides-derived, repetitive DNA sequences in diploid Aegilops species, wheat-Aegilops amphiploids and derived chromosome addition lines. Cytogenet Genome Res. 2010;129(1-3):47-54.
Kumar, S., Friebe, B., & Gill, B. S. (2010). Fate of Aegilops speltoides-derived, repetitive DNA sequences in diploid Aegilops species, wheat-Aegilops amphiploids and derived chromosome addition lines. Cytogenetic and Genome Research, 129(1-3), 47-54. https://doi.org/10.1159/000314552
Kumar S, Friebe B, Gill BS. Fate of Aegilops Speltoides-derived, Repetitive DNA Sequences in Diploid Aegilops Species, wheat-Aegilops Amphiploids and Derived Chromosome Addition Lines. Cytogenet Genome Res. 2010;129(1-3):47-54. PubMed PMID: 20551615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fate of Aegilops speltoides-derived, repetitive DNA sequences in diploid Aegilops species, wheat-Aegilops amphiploids and derived chromosome addition lines. AU - Kumar,S, AU - Friebe,B, AU - Gill,B S, Y1 - 2010/06/15/ PY - 2010/6/17/entrez PY - 2010/6/17/pubmed PY - 2010/8/7/medline SP - 47 EP - 54 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 129 IS - 1-3 N2 - The present study reports the cloning and characterization of an Aegilops speltoides-derived subtelomeric repeat, designated as pSp1B16. Clone pSp1B16 has 98% sequence homology with the previously isolated Ae. speltoides repeat Spelt1. The distribution of pSp1B16 and another Ae. speltoides repeat, pGc1R1, was analyzed in diploid Aegilops species, tetra- and hexaploid wheats, wheat-Aegilops amphiploids and derived chromosome addition lines by fluorescence in situ hybridization (FISH). Clones pSp1B16 and pGc1R1 revealed FISH sites in Ae. speltoides, Ae. sharonensis and Triticum timopheevii, whereas additional pGc1R1 FISH sites were observed in Ae. longissima and Ae. caudata. The pSp1B16 and pGc1R1 FISH patterns of the Aegilops chromosomes in the wheat-Aegilops amphiploids and chromosome addition lines are similar to those present in the Aegilops parent accession. We did not observe any evidence of pSp1B16 and pGc1R1 sequence elimination, which is in contrast to previous studies using similar hybrids and repeats. The presented data suggest that the genomic changes in synthetic amphiploids observed in previous studies might be caused by homoeologous recombination, which was suppressed in the amphiploid analyzed in this study. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/20551615/Fate_of_Aegilops_speltoides_derived_repetitive_DNA_sequences_in_diploid_Aegilops_species_wheat_Aegilops_amphiploids_and_derived_chromosome_addition_lines_ L2 - https://www.karger.com?DOI=10.1159/000314552 DB - PRIME DP - Unbound Medicine ER -