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A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers.
Cytogenet Genome Res. 2010 Jul; 129(1-3):224-33.CG

Abstract

The short arm of rye chromosome 1R (1RS) is introduced into many common wheat cultivars because of its agronomic importance. The gametocidal system has been used to produce dissection lines carrying segments of rye chromosome 1R. We focused on establishing more dissection lines for 1RS and on obtaining PCR-based markers specific to 1RS. We established 66 1RS dissection lines carrying 1RS segments of chromosome 1R derived from a common wheat cultivar 'Burgas 2' and obtained 27 markers. We conducted a PCR analysis using the dissection lines and markers, and divided 1RS into 17 regions separated by the breakpoints. Comparison of the 'Burgas 2' 1RS map with another map of 1RS derived from 'Imperial' rye implied a restructuring between the 2 1RS chromosomes.

Authors+Show Affiliations

Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20551617

Citation

Gyawali, Y P., et al. "A Cytological Map of the Short Arm of Rye Chromosome 1R Constructed With 1R Dissection Stocks of Common Wheat and PCR-based Markers." Cytogenetic and Genome Research, vol. 129, no. 1-3, 2010, pp. 224-33.
Gyawali YP, Nasuda S, Endo TR. A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers. Cytogenet Genome Res. 2010;129(1-3):224-33.
Gyawali, Y. P., Nasuda, S., & Endo, T. R. (2010). A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers. Cytogenetic and Genome Research, 129(1-3), 224-33. https://doi.org/10.1159/000314556
Gyawali YP, Nasuda S, Endo TR. A Cytological Map of the Short Arm of Rye Chromosome 1R Constructed With 1R Dissection Stocks of Common Wheat and PCR-based Markers. Cytogenet Genome Res. 2010;129(1-3):224-33. PubMed PMID: 20551617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers. AU - Gyawali,Y P, AU - Nasuda,S, AU - Endo,T R, Y1 - 2010/06/11/ PY - 2010/6/17/entrez PY - 2010/6/17/pubmed PY - 2010/8/7/medline SP - 224 EP - 33 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 129 IS - 1-3 N2 - The short arm of rye chromosome 1R (1RS) is introduced into many common wheat cultivars because of its agronomic importance. The gametocidal system has been used to produce dissection lines carrying segments of rye chromosome 1R. We focused on establishing more dissection lines for 1RS and on obtaining PCR-based markers specific to 1RS. We established 66 1RS dissection lines carrying 1RS segments of chromosome 1R derived from a common wheat cultivar 'Burgas 2' and obtained 27 markers. We conducted a PCR analysis using the dissection lines and markers, and divided 1RS into 17 regions separated by the breakpoints. Comparison of the 'Burgas 2' 1RS map with another map of 1RS derived from 'Imperial' rye implied a restructuring between the 2 1RS chromosomes. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/20551617/A_cytological_map_of_the_short_arm_of_rye_chromosome_1R_constructed_with_1R_dissection_stocks_of_common_wheat_and_PCR_based_markers_ L2 - https://www.karger.com?DOI=10.1159/000314556 DB - PRIME DP - Unbound Medicine ER -