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Neurotrophin-4 dependency of intraepithelial vagal sensory nerve terminals that selectively contact pulmonary NEBs in mice.
Histol Histopathol. 2010 08; 25(8):975-84.HH

Abstract

Important physiological functions of neurotrophins (NTs) in airways and lungs are the early development, differentiation and maintenance of peripheral sensory neurons. The main pulmonary sensory innervation is of vagal origin, with several nerve fibre populations that selectively contact complex morphologically well-characterized receptor end-organs, called neuroepithelial bodies (NEBs). NEBs in mouse lungs are innervated by at least two separate myelinated vagal sensory nerve fibre populations, of which the neurochemical coding is suggestive of a mechanosensory function. Since neurotrophin-4 (NT-4) has been especially described to be important for the maintenance of mechanosensory nerve terminals, the present study aimed at investigating the NT-4 dependency of the two myelinated vagal sensory nerve fibre populations innervating mouse pulmonary NEBs. Multiple immunostaining in 21-day-old and adult mouse lungs revealed the expression of the NT-4 receptor TrkB on the two different myelinated vagal sensory nerve fibre populations, i.e., the vesicular glutamate transporter/calbindin-positive and the P2X2/3-positive fibres, which selectively contact pulmonary NEBs. Examination of the effect of the lack of NT-4 on these NEB-related nerve fibre populations, by comparing adult NT-4-/- and wild-type mice, revealed that in NT-4-/- mice the percentage of NEBs contacted by P2X2/3+ is reduced by 75%, while the VGLUT+/CB+ population seemed to be unaffected. This study demonstrated that although mouse pulmonary NEBs are contacted by two distinct TrkB expressing populations of vagal myelinated afferents, only one is distinctly reduced in NT-4 deficient mice, suggesting the involvement of NTs. In view of the growing evidence for the involvement of NTs in neuronal plasticity associated with airway diseases, pulmonary NEBs innervated by NT-sensitive vagal afferents may play a significant role.

Authors+Show Affiliations

Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20552548

Citation

Oztay, Fusun, et al. "Neurotrophin-4 Dependency of Intraepithelial Vagal Sensory Nerve Terminals That Selectively Contact Pulmonary NEBs in Mice." Histology and Histopathology, vol. 25, no. 8, 2010, pp. 975-84.
Oztay F, Brouns I, Pintelon I, et al. Neurotrophin-4 dependency of intraepithelial vagal sensory nerve terminals that selectively contact pulmonary NEBs in mice. Histol Histopathol. 2010;25(8):975-84.
Oztay, F., Brouns, I., Pintelon, I., Raab, M., Neuhuber, W., Timmermans, J. P., & Adriaensen, D. (2010). Neurotrophin-4 dependency of intraepithelial vagal sensory nerve terminals that selectively contact pulmonary NEBs in mice. Histology and Histopathology, 25(8), 975-84. https://doi.org/10.14670/HH-25.975
Oztay F, et al. Neurotrophin-4 Dependency of Intraepithelial Vagal Sensory Nerve Terminals That Selectively Contact Pulmonary NEBs in Mice. Histol Histopathol. 2010;25(8):975-84. PubMed PMID: 20552548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neurotrophin-4 dependency of intraepithelial vagal sensory nerve terminals that selectively contact pulmonary NEBs in mice. AU - Oztay,Fusun, AU - Brouns,Inge, AU - Pintelon,Isabel, AU - Raab,Marion, AU - Neuhuber,Winfried, AU - Timmermans,Jean-Pierre, AU - Adriaensen,Dirk, PY - 2010/6/17/entrez PY - 2010/6/17/pubmed PY - 2010/9/24/medline SP - 975 EP - 84 JF - Histology and histopathology JO - Histol. Histopathol. VL - 25 IS - 8 N2 - Important physiological functions of neurotrophins (NTs) in airways and lungs are the early development, differentiation and maintenance of peripheral sensory neurons. The main pulmonary sensory innervation is of vagal origin, with several nerve fibre populations that selectively contact complex morphologically well-characterized receptor end-organs, called neuroepithelial bodies (NEBs). NEBs in mouse lungs are innervated by at least two separate myelinated vagal sensory nerve fibre populations, of which the neurochemical coding is suggestive of a mechanosensory function. Since neurotrophin-4 (NT-4) has been especially described to be important for the maintenance of mechanosensory nerve terminals, the present study aimed at investigating the NT-4 dependency of the two myelinated vagal sensory nerve fibre populations innervating mouse pulmonary NEBs. Multiple immunostaining in 21-day-old and adult mouse lungs revealed the expression of the NT-4 receptor TrkB on the two different myelinated vagal sensory nerve fibre populations, i.e., the vesicular glutamate transporter/calbindin-positive and the P2X2/3-positive fibres, which selectively contact pulmonary NEBs. Examination of the effect of the lack of NT-4 on these NEB-related nerve fibre populations, by comparing adult NT-4-/- and wild-type mice, revealed that in NT-4-/- mice the percentage of NEBs contacted by P2X2/3+ is reduced by 75%, while the VGLUT+/CB+ population seemed to be unaffected. This study demonstrated that although mouse pulmonary NEBs are contacted by two distinct TrkB expressing populations of vagal myelinated afferents, only one is distinctly reduced in NT-4 deficient mice, suggesting the involvement of NTs. In view of the growing evidence for the involvement of NTs in neuronal plasticity associated with airway diseases, pulmonary NEBs innervated by NT-sensitive vagal afferents may play a significant role. SN - 1699-5848 UR - https://www.unboundmedicine.com/medline/citation/20552548/Neurotrophin_4_dependency_of_intraepithelial_vagal_sensory_nerve_terminals_that_selectively_contact_pulmonary_NEBs_in_mice_ L2 - http://www.hh.um.es/Abstracts/Vol_25/25_8/25_8_975.htm DB - PRIME DP - Unbound Medicine ER -