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Production and identification of wheat - Agropyron cristatum (1.4P) alien translocation lines.
Genome. 2010 Jun; 53(6):472-81.G

Abstract

The P genome of Agropyron Gaertn., a wild relative of wheat, contains an abundance of desirable genes that can be utilized as genetic resources to improve wheat. In this study, wheat - Aegilops cylindrica Host gametocidal chromosome 2C addition lines were crossed with wheat - Agropyron cristatum (L.) Gaertn. disomic addition line accession II-21 with alien recombinant chromosome (1.4)P. We successfully induced wheat - A. cristatum alien chromosomal translocations for the first time. The frequency of translocation in the progeny was 3.75%, which was detected by molecular markers and genomic in situ hybridization (GISH). The translocation chromosomes were identified by dual-color GISH /fluorescence in situ hybridization (FISH). The P genomic DNA was used as probe to detect the (1.4)P chromosome fragment, and pHvG39, pAs1, or pSc119.2 repeated sequences were used as probes to identify wheat translocated chromosomes. The results showed that six types of translocations were identified in the three wheat - A. cristatum alien translocation lines, including the whole arm or terminal portion of a (1.4)P chromosome. The (1.4)P chromosome fragments were translocated to wheat chromosomes 1B, 2B, 5B, and 3D. The breakpoints were located at the centromeres of 1B and 2B, the pericentric locations of 5BS, and the terminals of 5BL and 3DS. In addition, we obtained 12 addition-deletion lines that contained alien A. cristatum chromosome (1.4)P in wheat background. All of these wheat - A. cristatum alien translocation lines and addition-deletion lines would be valuable for identifying A. cristatum chromosome (1.4)P-related genes and providing genetic resources and new germplasm accessions for the genetic improvement of wheat. The specific molecular markers of A. cristatum (1.4)P chromosome have been developed and used to track the (1.4)P chromatin.

Authors+Show Affiliations

National Key Facilities for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20555436

Citation

Liu, Wei-Hua, et al. "Production and Identification of Wheat - Agropyron Cristatum (1.4P) Alien Translocation Lines." Genome, vol. 53, no. 6, 2010, pp. 472-81.
Liu WH, Luan Y, Wang JC, et al. Production and identification of wheat - Agropyron cristatum (1.4P) alien translocation lines. Genome. 2010;53(6):472-81.
Liu, W. H., Luan, Y., Wang, J. C., Wang, X. G., Su, J. J., Zhang, J. P., Yang, X. M., Gao, A. N., & Li, L. H. (2010). Production and identification of wheat - Agropyron cristatum (1.4P) alien translocation lines. Genome, 53(6), 472-81. https://doi.org/10.1139/g10-023
Liu WH, et al. Production and Identification of Wheat - Agropyron Cristatum (1.4P) Alien Translocation Lines. Genome. 2010;53(6):472-81. PubMed PMID: 20555436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production and identification of wheat - Agropyron cristatum (1.4P) alien translocation lines. AU - Liu,Wei-Hua, AU - Luan,Yang, AU - Wang,Jing-Chang, AU - Wang,Xiao-Guang, AU - Su,Jun-Ji, AU - Zhang,Jin-Peng, AU - Yang,Xin-Ming, AU - Gao,Ai-Nong, AU - Li,Li-Hui, PY - 2010/6/18/entrez PY - 2010/6/18/pubmed PY - 2010/9/29/medline SP - 472 EP - 81 JF - Genome JO - Genome VL - 53 IS - 6 N2 - The P genome of Agropyron Gaertn., a wild relative of wheat, contains an abundance of desirable genes that can be utilized as genetic resources to improve wheat. In this study, wheat - Aegilops cylindrica Host gametocidal chromosome 2C addition lines were crossed with wheat - Agropyron cristatum (L.) Gaertn. disomic addition line accession II-21 with alien recombinant chromosome (1.4)P. We successfully induced wheat - A. cristatum alien chromosomal translocations for the first time. The frequency of translocation in the progeny was 3.75%, which was detected by molecular markers and genomic in situ hybridization (GISH). The translocation chromosomes were identified by dual-color GISH /fluorescence in situ hybridization (FISH). The P genomic DNA was used as probe to detect the (1.4)P chromosome fragment, and pHvG39, pAs1, or pSc119.2 repeated sequences were used as probes to identify wheat translocated chromosomes. The results showed that six types of translocations were identified in the three wheat - A. cristatum alien translocation lines, including the whole arm or terminal portion of a (1.4)P chromosome. The (1.4)P chromosome fragments were translocated to wheat chromosomes 1B, 2B, 5B, and 3D. The breakpoints were located at the centromeres of 1B and 2B, the pericentric locations of 5BS, and the terminals of 5BL and 3DS. In addition, we obtained 12 addition-deletion lines that contained alien A. cristatum chromosome (1.4)P in wheat background. All of these wheat - A. cristatum alien translocation lines and addition-deletion lines would be valuable for identifying A. cristatum chromosome (1.4)P-related genes and providing genetic resources and new germplasm accessions for the genetic improvement of wheat. The specific molecular markers of A. cristatum (1.4)P chromosome have been developed and used to track the (1.4)P chromatin. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/20555436/Production_and_identification_of_wheat___Agropyron_cristatum__1_4P__alien_translocation_lines_ L2 - https://cdnsciencepub.com/doi/10.1139/g10-023?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -