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First toxin profile of ciguateric fish in Madeira Arquipelago (Europe).
Anal Chem. 2010 Jul 15; 82(14):6032-9.AC

Abstract

Ciguatera fish poisoning (CFP) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (CTXs). These toxins are issued from Gambierdiscus species of dinoflagellates. Herbivorous fish accumulate these toxins in their musculature and viscera after ingesting dinoflagellates. Epidemiological studies showed that CFP has been present in areas between 35 degrees North and 35 degrees South latitude, mainly, Indo-pacific and Caribbean areas, but not in waters closed to European and African continent. In the present paper, a specimen of Seriola dumerili weighing 70 kg and a smaller Seriola fasciata specimen, captured in waters belonging to Selvagens Islands (Madeira Arquipelago), were analyzed. Fishes from this genus were implicated in previous suspected ciguatera poisoning outbreaks in the Portuguese Madeira Arquipelago in the North Atlantic Ocean. Analysis was performed by two approaches, a functional method using cerebellar granule cells and by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) method. The study was carried out in one portion of the tail muscle of Seriola fasciata and five parts of the body of Seriola dumerili (tail muscle, head, ventral muscle, mid muscle, and liver). The functional method consisted in the modification of the inward sodium current in cerebellar granule cells and the chemical method was a high resolution chromatography, which allowed elucidating the toxin profile in the samples. In addition, UPLC-MS technique was optimized and used for detecting and quantifying CTXs for the first time. After fish extraction and clean up, the chromatograms revealed the presence of CTX-1B at 1111.6 m/z, CTX-3C at 1023.5 m/z, a CTX analogue at 1040.6 m/z, and a CTX from the Caribbean or Indic waters at 1141.6 m/z. Therefore, the results obtained in the present paper for both methods confirm, for the first time, the presence of CTX in fish from Madeira Arquipelago.

Authors+Show Affiliations

Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20557036

Citation

Otero, Paz, et al. "First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe)." Analytical Chemistry, vol. 82, no. 14, 2010, pp. 6032-9.
Otero P, Pérez S, Alfonso A, et al. First toxin profile of ciguateric fish in Madeira Arquipelago (Europe). Anal Chem. 2010;82(14):6032-9.
Otero, P., Pérez, S., Alfonso, A., Vale, C., Rodríguez, P., Gouveia, N. N., Gouveia, N., Delgado, J., Vale, P., Hirama, M., Ishihara, Y., Molgó, J., & Botana, L. M. (2010). First toxin profile of ciguateric fish in Madeira Arquipelago (Europe). Analytical Chemistry, 82(14), 6032-9. https://doi.org/10.1021/ac100516q
Otero P, et al. First Toxin Profile of Ciguateric Fish in Madeira Arquipelago (Europe). Anal Chem. 2010 Jul 15;82(14):6032-9. PubMed PMID: 20557036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First toxin profile of ciguateric fish in Madeira Arquipelago (Europe). AU - Otero,Paz, AU - Pérez,Sheila, AU - Alfonso,Amparo, AU - Vale,Carmen, AU - Rodríguez,Paula, AU - Gouveia,Neide N, AU - Gouveia,Nuno, AU - Delgado,João, AU - Vale,Paulo, AU - Hirama,Masahiro, AU - Ishihara,Yuuki, AU - Molgó,Jordi, AU - Botana,Luis M, PY - 2010/6/19/entrez PY - 2010/6/19/pubmed PY - 2010/11/5/medline SP - 6032 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 82 IS - 14 N2 - Ciguatera fish poisoning (CFP) is a human foodborne intoxication caused by ingestion of tropical fishes contaminated with the potent polyether toxins known as ciguatoxins (CTXs). These toxins are issued from Gambierdiscus species of dinoflagellates. Herbivorous fish accumulate these toxins in their musculature and viscera after ingesting dinoflagellates. Epidemiological studies showed that CFP has been present in areas between 35 degrees North and 35 degrees South latitude, mainly, Indo-pacific and Caribbean areas, but not in waters closed to European and African continent. In the present paper, a specimen of Seriola dumerili weighing 70 kg and a smaller Seriola fasciata specimen, captured in waters belonging to Selvagens Islands (Madeira Arquipelago), were analyzed. Fishes from this genus were implicated in previous suspected ciguatera poisoning outbreaks in the Portuguese Madeira Arquipelago in the North Atlantic Ocean. Analysis was performed by two approaches, a functional method using cerebellar granule cells and by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) method. The study was carried out in one portion of the tail muscle of Seriola fasciata and five parts of the body of Seriola dumerili (tail muscle, head, ventral muscle, mid muscle, and liver). The functional method consisted in the modification of the inward sodium current in cerebellar granule cells and the chemical method was a high resolution chromatography, which allowed elucidating the toxin profile in the samples. In addition, UPLC-MS technique was optimized and used for detecting and quantifying CTXs for the first time. After fish extraction and clean up, the chromatograms revealed the presence of CTX-1B at 1111.6 m/z, CTX-3C at 1023.5 m/z, a CTX analogue at 1040.6 m/z, and a CTX from the Caribbean or Indic waters at 1141.6 m/z. Therefore, the results obtained in the present paper for both methods confirm, for the first time, the presence of CTX in fish from Madeira Arquipelago. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/20557036/First_toxin_profile_of_ciguateric_fish_in_Madeira_Arquipelago__Europe__ L2 - https://doi.org/10.1021/ac100516q DB - PRIME DP - Unbound Medicine ER -