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Carbohydrate-to-fat ratio affects food intake and body weight in Wistar rats.
Exp Biol Med (Maywood). 2010 Jul; 235(7):833-8.EB

Abstract

The aim of the study was to evaluate the impact of carbohydrate-to-fat ratio on body weight and appetite regulation in Wistar rats. Twenty-four Wistar rats were randomized to three dietary groups (n = 8): normal carbohydrate diet (NC), low-carbohydrate diet (LC) and high-carbohydrate diet (HC) for 12 weeks. Body weight and food intake were recorded. Circulating leptin and insulin levels were measured by radioimmunoassay method. The expression levels of leptin receptor, insulin receptor, orexin, neuropeptide Y (NPY), agouti-related protein (AgRP) and melanocortin-4 receptor (MC-4R) in the hypothalamus were also measured by realtime polymerase chain reaction (PCR). In the LC group, food intake reduced while body weight increased significantly compared with the NC and HC groups. Plasma leptin levels increased in the LC (18.5 +/- 8.2 ng/mL) group compared with the NC (8.6 +/- 3.8 ng/mL, P < 0.001) and HC (6.6 +/- 1.9 ng/mL, P < 0.001) groups. Realtime reverse transcription-PCR revealed a decrease in the hypothalamic expression level of only leptin receptor in the LC (0.764, 0.471-4.648 copy/mL) and HC (0.357, 0.129-0.781 copy/mL) groups compared with the NC (1.323, 0.616-2.392 copy/mL; P = 0.01) group, and that there was no significant change in those of insulin receptor, AgRP, Orexin, NPY and MC-4R. Low-carbohydrate, high-fat diet raised body weight, which led to a rising of circulating leptin levels and a reduced expression of leptin receptor in the hypothalamus.

Authors+Show Affiliations

Department of Clinical Nutrition, Ren Ji Hospital and Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20558837

Citation

Xu, Ren-Ying, et al. "Carbohydrate-to-fat Ratio Affects Food Intake and Body Weight in Wistar Rats." Experimental Biology and Medicine (Maywood, N.J.), vol. 235, no. 7, 2010, pp. 833-8.
Xu RY, Wan YP, Tang QY, et al. Carbohydrate-to-fat ratio affects food intake and body weight in Wistar rats. Exp Biol Med (Maywood). 2010;235(7):833-8.
Xu, R. Y., Wan, Y. P., Tang, Q. Y., Wu, J., & Cai, W. (2010). Carbohydrate-to-fat ratio affects food intake and body weight in Wistar rats. Experimental Biology and Medicine (Maywood, N.J.), 235(7), 833-8. https://doi.org/10.1258/ebm.2010.009276
Xu RY, et al. Carbohydrate-to-fat Ratio Affects Food Intake and Body Weight in Wistar Rats. Exp Biol Med (Maywood). 2010;235(7):833-8. PubMed PMID: 20558837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbohydrate-to-fat ratio affects food intake and body weight in Wistar rats. AU - Xu,Ren-Ying, AU - Wan,Yan-Ping, AU - Tang,Qing-Ya, AU - Wu,Jiang, AU - Cai,Wei, PY - 2010/6/19/entrez PY - 2010/6/19/pubmed PY - 2010/7/10/medline SP - 833 EP - 8 JF - Experimental biology and medicine (Maywood, N.J.) JO - Exp. Biol. Med. (Maywood) VL - 235 IS - 7 N2 - The aim of the study was to evaluate the impact of carbohydrate-to-fat ratio on body weight and appetite regulation in Wistar rats. Twenty-four Wistar rats were randomized to three dietary groups (n = 8): normal carbohydrate diet (NC), low-carbohydrate diet (LC) and high-carbohydrate diet (HC) for 12 weeks. Body weight and food intake were recorded. Circulating leptin and insulin levels were measured by radioimmunoassay method. The expression levels of leptin receptor, insulin receptor, orexin, neuropeptide Y (NPY), agouti-related protein (AgRP) and melanocortin-4 receptor (MC-4R) in the hypothalamus were also measured by realtime polymerase chain reaction (PCR). In the LC group, food intake reduced while body weight increased significantly compared with the NC and HC groups. Plasma leptin levels increased in the LC (18.5 +/- 8.2 ng/mL) group compared with the NC (8.6 +/- 3.8 ng/mL, P < 0.001) and HC (6.6 +/- 1.9 ng/mL, P < 0.001) groups. Realtime reverse transcription-PCR revealed a decrease in the hypothalamic expression level of only leptin receptor in the LC (0.764, 0.471-4.648 copy/mL) and HC (0.357, 0.129-0.781 copy/mL) groups compared with the NC (1.323, 0.616-2.392 copy/mL; P = 0.01) group, and that there was no significant change in those of insulin receptor, AgRP, Orexin, NPY and MC-4R. Low-carbohydrate, high-fat diet raised body weight, which led to a rising of circulating leptin levels and a reduced expression of leptin receptor in the hypothalamus. SN - 1535-3699 UR - https://www.unboundmedicine.com/medline/citation/20558837/Carbohydrate_to_fat_ratio_affects_food_intake_and_body_weight_in_Wistar_rats_ L2 - http://journals.sagepub.com/doi/full/10.1258/ebm.2010.009276?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -