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Lymphocytic bronchiolitis is associated with inadequate suppression of blood T-cell granzyme B, IFN-gamma, and TNF-alpha.
Transplantation. 2010 May 27; 89(10):1283-9.T

Abstract

BACKGROUND

Lymphocytic bronchiolitis (LB) has been shown to be an important factor for the subsequent development of obliterative bronchiolitis (OB). We have previously shown that OB, which limits long-term survival after lung transplantation, is associated with lack of suppression of peripheral blood T-cell granzyme B, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha. However, the role of these proinflammatory mediators in LB is unknown. We hypothesized that these proinflammatory mediators may also be increased during LB episodes despite standard immunosuppression regimens.

METHODS

T-cell intracellular cytokine profiles and granzyme B were studied in whole blood, bronchoalveolar lavage samples, and bronchial brushings from stable lung transplant patients with LB and from healthy controls, using multiparameter flow cytometry.

RESULTS

There was a significant increase in peripheral blood T-cell granzyme B and CD8 T-cell IFN-gamma and TNF-alpha in patients with LB compared with control and stable groups and a decrease in CD25CD127CD3CD8 T regulatory cells in stable and LB transplant patients compared with controls. No changes were noted in the airways.

CONCLUSIONS

LB is associated with inadequate suppression of peripheral blood T-cell granzyme B, IFN-gamma, and TNF-alpha. Drugs that effectively reduce these proinflammatory mediators may improve current protocols for treating LB and possibly reduce subsequent progression to OB in lung transplant patients.

Authors+Show Affiliations

Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, South Australia, Australia. greg.hodge@health.sa.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20559033

Citation

Hodge, Greg, et al. "Lymphocytic Bronchiolitis Is Associated With Inadequate Suppression of Blood T-cell Granzyme B, IFN-gamma, and TNF-alpha." Transplantation, vol. 89, no. 10, 2010, pp. 1283-9.
Hodge G, Hodge S, Li-Liew C, et al. Lymphocytic bronchiolitis is associated with inadequate suppression of blood T-cell granzyme B, IFN-gamma, and TNF-alpha. Transplantation. 2010;89(10):1283-9.
Hodge, G., Hodge, S., Li-Liew, C., Chambers, D., Hopkins, P., Reynolds, P. N., & Holmes, M. (2010). Lymphocytic bronchiolitis is associated with inadequate suppression of blood T-cell granzyme B, IFN-gamma, and TNF-alpha. Transplantation, 89(10), 1283-9. https://doi.org/10.1097/TP.0b013e3181d75971
Hodge G, et al. Lymphocytic Bronchiolitis Is Associated With Inadequate Suppression of Blood T-cell Granzyme B, IFN-gamma, and TNF-alpha. Transplantation. 2010 May 27;89(10):1283-9. PubMed PMID: 20559033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lymphocytic bronchiolitis is associated with inadequate suppression of blood T-cell granzyme B, IFN-gamma, and TNF-alpha. AU - Hodge,Greg, AU - Hodge,Sandra, AU - Li-Liew,Chien, AU - Chambers,Daniel, AU - Hopkins,Peter, AU - Reynolds,Paul N, AU - Holmes,Mark, PY - 2010/6/19/entrez PY - 2010/6/19/pubmed PY - 2010/7/8/medline SP - 1283 EP - 9 JF - Transplantation JO - Transplantation VL - 89 IS - 10 N2 - BACKGROUND: Lymphocytic bronchiolitis (LB) has been shown to be an important factor for the subsequent development of obliterative bronchiolitis (OB). We have previously shown that OB, which limits long-term survival after lung transplantation, is associated with lack of suppression of peripheral blood T-cell granzyme B, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha. However, the role of these proinflammatory mediators in LB is unknown. We hypothesized that these proinflammatory mediators may also be increased during LB episodes despite standard immunosuppression regimens. METHODS: T-cell intracellular cytokine profiles and granzyme B were studied in whole blood, bronchoalveolar lavage samples, and bronchial brushings from stable lung transplant patients with LB and from healthy controls, using multiparameter flow cytometry. RESULTS: There was a significant increase in peripheral blood T-cell granzyme B and CD8 T-cell IFN-gamma and TNF-alpha in patients with LB compared with control and stable groups and a decrease in CD25CD127CD3CD8 T regulatory cells in stable and LB transplant patients compared with controls. No changes were noted in the airways. CONCLUSIONS: LB is associated with inadequate suppression of peripheral blood T-cell granzyme B, IFN-gamma, and TNF-alpha. Drugs that effectively reduce these proinflammatory mediators may improve current protocols for treating LB and possibly reduce subsequent progression to OB in lung transplant patients. SN - 1534-6080 UR - https://www.unboundmedicine.com/medline/citation/20559033/Lymphocytic_bronchiolitis_is_associated_with_inadequate_suppression_of_blood_T_cell_granzyme_B_IFN_gamma_and_TNF_alpha_ L2 - http://dx.doi.org/10.1097/TP.0b013e3181d75971 DB - PRIME DP - Unbound Medicine ER -