Tags

Type your tag names separated by a space and hit enter

Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
J Pharm Biomed Anal. 2010 Nov 02; 53(3):462-74.JP

Abstract

Steroidal saponins are the major bioactive constituents of Dioscorea zingiberensis C. H. Wright (D. zingiberensis). In this work, ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS/MS) was applied to the separation and characterization of steroidal saponins in crude extracts from D. zingiberensis. The results showed that fragment ions from glycosidic and cross-ring cleavages gave a wealth of structural information related to aglycone skeletons, sugar types and the sequence of sugar units. According to the summarized fragmentation patterns, identification of steroidal saponins from D. zingiberensis could be fulfilled, even when reference standards were unavailable. As a result, a total of thirty-one saponins with five aglycone skeletons, including fourteen new trace saponins, were identified or tentatively elucidated in crude extracts from D. zingiberensis based on their retention times, the mass spectrometric fragmentation patterns, and MS and MS/MS data.

Authors+Show Affiliations

Institute of Chemistry and Applications of Plant Resource, School of Biological & Food Engineering, Dalian Polytechnic University, 1 Qinggong Yuan, Ganjingzi District, Dalian 116034, China. zhujingb@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20561762

Citation

Zhu, Jingbo, et al. "Characterization of Steroidal Saponins in Crude Extracts From Dioscorea Zingiberensis C. H. Wright By Ultra-performance Liquid Chromatography/electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 53, no. 3, 2010, pp. 462-74.
Zhu J, Guo X, Fu S, et al. Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. J Pharm Biomed Anal. 2010;53(3):462-74.
Zhu, J., Guo, X., Fu, S., Zhang, X., & Liang, X. (2010). Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 53(3), 462-74. https://doi.org/10.1016/j.jpba.2010.05.019
Zhu J, et al. Characterization of Steroidal Saponins in Crude Extracts From Dioscorea Zingiberensis C. H. Wright By Ultra-performance Liquid Chromatography/electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry. J Pharm Biomed Anal. 2010 Nov 2;53(3):462-74. PubMed PMID: 20561762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of steroidal saponins in crude extracts from Dioscorea zingiberensis C. H. Wright by ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. AU - Zhu,Jingbo, AU - Guo,Xiujie, AU - Fu,Shaoping, AU - Zhang,Xiuli, AU - Liang,Xinmiao, Y1 - 2010/05/27/ PY - 2009/12/04/received PY - 2010/05/17/revised PY - 2010/05/19/accepted PY - 2010/6/22/entrez PY - 2010/6/22/pubmed PY - 2010/9/18/medline SP - 462 EP - 74 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 53 IS - 3 N2 - Steroidal saponins are the major bioactive constituents of Dioscorea zingiberensis C. H. Wright (D. zingiberensis). In this work, ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS/MS) was applied to the separation and characterization of steroidal saponins in crude extracts from D. zingiberensis. The results showed that fragment ions from glycosidic and cross-ring cleavages gave a wealth of structural information related to aglycone skeletons, sugar types and the sequence of sugar units. According to the summarized fragmentation patterns, identification of steroidal saponins from D. zingiberensis could be fulfilled, even when reference standards were unavailable. As a result, a total of thirty-one saponins with five aglycone skeletons, including fourteen new trace saponins, were identified or tentatively elucidated in crude extracts from D. zingiberensis based on their retention times, the mass spectrometric fragmentation patterns, and MS and MS/MS data. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/20561762/Characterization_of_steroidal_saponins_in_crude_extracts_from_Dioscorea_zingiberensis_C__H__Wright_by_ultra_performance_liquid_chromatography/electrospray_ionization_quadrupole_time_of_flight_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -