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Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells.
J Ethnopharmacol. 2010 Aug 09; 130(3):536-44.JE

Abstract

AIM OF THE STUDY

Ardisia species, notably A. compressa, are used in some regions of the world as food or in traditional medicine for prevention and treatment of certain health conditions including liver disease. We investigated the chemical composition and relative anticancer potential of six Ardisia species [A. japonica (AJ), A. escallonioides (AES), A. mamillata (AM), A. compressa (AC), A. crenata (ACR), and A. elliptica (AE)].

MATERIALS AND METHODS

Antioxidant capacity, DNA human topoisomerase II catalytic inhibition, and cytotoxicity on human liver cancer cells (HepG2) were determined in vitro in tea extracts of the 6 Ardisia species evaluated. Selected pure phenolic compounds present in Ardisia species were also evaluated.

RESULTS

AC showed the highest topoisomerase II catalytic inhibition (IC(50)=12 microg/ml) and cytotoxicity (IC(50)=117 microg/ml) against HepG2 cells, followed by ACR and AJ. Total polyphenols ranged from 21 to 72 mg equivalents of gallic acid (GA)/g solid extract (SE). LC-MS analysis revealed the presence of GA, quercetin derivatives, ardisenone, ardisiaquinone, ardisianone, bergenin, norbergenin, and embelin. However, neither total polyphenol concentration nor antioxidant capacity correlated with anticancer capacity. Significant HepG2 cytotoxicity was also achieved by bergenin (IC(50)=18 microM) and embelin (IC(50)=120 microM). AC, bergenin, embelin, and quercetin showed a tendency to accumulate cells in the G1 phase and reduced G2/M leading to apoptosis.

CONCLUSIONS

Although the mechanism is not entirely clear, AC, ACR, and AJ are the Ardisia species with the greatest anticancer potential against liver cancer cells in vitro and deserve further investigation.

Authors+Show Affiliations

Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, IL, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20561930

Citation

Newell, Amanda M B., et al. "Comparative in Vitro Bioactivities of Tea Extracts From Six Species of Ardisia and Their Effect On Growth Inhibition of HepG2 Cells." Journal of Ethnopharmacology, vol. 130, no. 3, 2010, pp. 536-44.
Newell AM, Yousef GG, Lila MA, et al. Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. J Ethnopharmacol. 2010;130(3):536-44.
Newell, A. M., Yousef, G. G., Lila, M. A., Ramírez-Mares, M. V., & de Mejia, E. G. (2010). Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. Journal of Ethnopharmacology, 130(3), 536-44. https://doi.org/10.1016/j.jep.2010.05.051
Newell AM, et al. Comparative in Vitro Bioactivities of Tea Extracts From Six Species of Ardisia and Their Effect On Growth Inhibition of HepG2 Cells. J Ethnopharmacol. 2010 Aug 9;130(3):536-44. PubMed PMID: 20561930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. AU - Newell,Amanda M B, AU - Yousef,Gad G, AU - Lila,Mary Ann, AU - Ramírez-Mares,Marco Vinicio, AU - de Mejia,Elvira Gonzalez, Y1 - 2010/06/02/ PY - 2010/02/20/received PY - 2010/05/12/revised PY - 2010/05/25/accepted PY - 2010/6/22/entrez PY - 2010/6/22/pubmed PY - 2010/11/4/medline SP - 536 EP - 44 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 130 IS - 3 N2 - AIM OF THE STUDY: Ardisia species, notably A. compressa, are used in some regions of the world as food or in traditional medicine for prevention and treatment of certain health conditions including liver disease. We investigated the chemical composition and relative anticancer potential of six Ardisia species [A. japonica (AJ), A. escallonioides (AES), A. mamillata (AM), A. compressa (AC), A. crenata (ACR), and A. elliptica (AE)]. MATERIALS AND METHODS: Antioxidant capacity, DNA human topoisomerase II catalytic inhibition, and cytotoxicity on human liver cancer cells (HepG2) were determined in vitro in tea extracts of the 6 Ardisia species evaluated. Selected pure phenolic compounds present in Ardisia species were also evaluated. RESULTS: AC showed the highest topoisomerase II catalytic inhibition (IC(50)=12 microg/ml) and cytotoxicity (IC(50)=117 microg/ml) against HepG2 cells, followed by ACR and AJ. Total polyphenols ranged from 21 to 72 mg equivalents of gallic acid (GA)/g solid extract (SE). LC-MS analysis revealed the presence of GA, quercetin derivatives, ardisenone, ardisiaquinone, ardisianone, bergenin, norbergenin, and embelin. However, neither total polyphenol concentration nor antioxidant capacity correlated with anticancer capacity. Significant HepG2 cytotoxicity was also achieved by bergenin (IC(50)=18 microM) and embelin (IC(50)=120 microM). AC, bergenin, embelin, and quercetin showed a tendency to accumulate cells in the G1 phase and reduced G2/M leading to apoptosis. CONCLUSIONS: Although the mechanism is not entirely clear, AC, ACR, and AJ are the Ardisia species with the greatest anticancer potential against liver cancer cells in vitro and deserve further investigation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20561930/Comparative_in_vitro_bioactivities_of_tea_extracts_from_six_species_of_Ardisia_and_their_effect_on_growth_inhibition_of_HepG2_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00371-5 DB - PRIME DP - Unbound Medicine ER -