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Protective effects of tetrahydropalmatine against gamma-radiation induced damage to human endothelial cells.
Life Sci. 2010 Jul 03; 87(1-2):55-63.LS

Abstract

AIMS

Irradiation-induced damage to pulmonary endothelial cells is thought to be an important mediator of the pathogenesis of radiation pneumonopathy. Tetrahydropalmatine (THP) has been shown to have a protective effect against oxidative stress. This study was designed to investigate the potential radioprotective effect of THP against irradiation-induced endothelial cellular damage and to elucidate the underlying mechanisms.

MAIN METHODS

Human EA.hy926 cells were treated with THP and irradiation. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For the detection of apoptosis, morphological observation, flow cytometry and a caspase-3 activity assay were employed. The expression of cytochrome-c and Bax/Bcl-2 protein were detected by western blot analysis. Generation of reactive oxygen species (ROS) was measured by flow cytometry. Malondialdehyde (MDA), lactate dehydrogenase (LDH), glutathione (GSH) and superoxide dismutase (SOD) were measured to assess cellular oxidative stress induced injury.

KEY FINDINGS

Preincubation of EA.hy926 cells with THP before gamma-radiation resulted in significant inhibition of apoptosis and enhancement of cell viability, as revealed by morphological observation, flow cytometry and MTT assay. THP significantly reduced intracellular ROS formation, levels of intracellular MDA and LDH, and enhanced the production of intracellular antioxidants (GSH and SOD) in EA.hy926 cells. Meanwhile, THP also inhibited the decrease of intracellular mitochondrial membrane potential (psim), caspase-3 activation, cytochrome-c release and reduced Bax/Bcl-2 ratio in THP pretreated, irradiated cells.

SIGNIFICANCE

Our findings demonstrated THP could effectively protect endothelial cells against gamma-irradiation injury, which could potentially be applied to the prevention of endothelial cell dysfunctions associated with ionizing irradiation-induced lung injury.

Authors+Show Affiliations

Department of Oncology, Guang 'anmen Hospital, China Academy of Chinese Medical Sciences, 5 Beixiange Road, Xuanwu District, Beijing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20562023

Citation

Yu, Jing, et al. "Protective Effects of Tetrahydropalmatine Against Gamma-radiation Induced Damage to Human Endothelial Cells." Life Sciences, vol. 87, no. 1-2, 2010, pp. 55-63.
Yu J, Piao BK, Pei YX, et al. Protective effects of tetrahydropalmatine against gamma-radiation induced damage to human endothelial cells. Life Sci. 2010;87(1-2):55-63.
Yu, J., Piao, B. K., Pei, Y. X., Qi, X., & Hua, B. J. (2010). Protective effects of tetrahydropalmatine against gamma-radiation induced damage to human endothelial cells. Life Sciences, 87(1-2), 55-63. https://doi.org/10.1016/j.lfs.2010.05.011
Yu J, et al. Protective Effects of Tetrahydropalmatine Against Gamma-radiation Induced Damage to Human Endothelial Cells. Life Sci. 2010 Jul 3;87(1-2):55-63. PubMed PMID: 20562023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of tetrahydropalmatine against gamma-radiation induced damage to human endothelial cells. AU - Yu,Jing, AU - Piao,Bin-kui, AU - Pei,Ying-xia, AU - Qi,Xin, AU - Hua,Bao-jin, Y1 - 2010/05/26/ PY - 2010/01/08/received PY - 2010/05/05/revised PY - 2010/05/18/accepted PY - 2010/6/22/entrez PY - 2010/6/22/pubmed PY - 2010/7/22/medline SP - 55 EP - 63 JF - Life sciences JO - Life Sci. VL - 87 IS - 1-2 N2 - AIMS: Irradiation-induced damage to pulmonary endothelial cells is thought to be an important mediator of the pathogenesis of radiation pneumonopathy. Tetrahydropalmatine (THP) has been shown to have a protective effect against oxidative stress. This study was designed to investigate the potential radioprotective effect of THP against irradiation-induced endothelial cellular damage and to elucidate the underlying mechanisms. MAIN METHODS: Human EA.hy926 cells were treated with THP and irradiation. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. For the detection of apoptosis, morphological observation, flow cytometry and a caspase-3 activity assay were employed. The expression of cytochrome-c and Bax/Bcl-2 protein were detected by western blot analysis. Generation of reactive oxygen species (ROS) was measured by flow cytometry. Malondialdehyde (MDA), lactate dehydrogenase (LDH), glutathione (GSH) and superoxide dismutase (SOD) were measured to assess cellular oxidative stress induced injury. KEY FINDINGS: Preincubation of EA.hy926 cells with THP before gamma-radiation resulted in significant inhibition of apoptosis and enhancement of cell viability, as revealed by morphological observation, flow cytometry and MTT assay. THP significantly reduced intracellular ROS formation, levels of intracellular MDA and LDH, and enhanced the production of intracellular antioxidants (GSH and SOD) in EA.hy926 cells. Meanwhile, THP also inhibited the decrease of intracellular mitochondrial membrane potential (psim), caspase-3 activation, cytochrome-c release and reduced Bax/Bcl-2 ratio in THP pretreated, irradiated cells. SIGNIFICANCE: Our findings demonstrated THP could effectively protect endothelial cells against gamma-irradiation injury, which could potentially be applied to the prevention of endothelial cell dysfunctions associated with ionizing irradiation-induced lung injury. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/20562023/Protective_effects_of_tetrahydropalmatine_against_gamma_radiation_induced_damage_to_human_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(10)00220-1 DB - PRIME DP - Unbound Medicine ER -