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Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination.
Clin Respir J. 2010 Apr; 4(2):83-8.CR

Abstract

BACKGROUND AND AIMS

Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not.

METHODS

EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity.

RESULTS

No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum.

CONCLUSION

We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination.

Authors+Show Affiliations

Respiratory Medicine and Allergology, Department of Clinical Sciences in Lund, Lund University, Lund, Sweden. Ellen.Tufvesson@med.lu.seNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20565481

Citation

Tufvesson, Ellen, et al. "Levels of Cysteinyl-leukotrienes in Exhaled Breath Condensate Are Not Due to Saliva Contamination." The Clinical Respiratory Journal, vol. 4, no. 2, 2010, pp. 83-8.
Tufvesson E, van Weele LJ, Ekedahl H, et al. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. Clin Respir J. 2010;4(2):83-8.
Tufvesson, E., van Weele, L. J., Ekedahl, H., & Bjermer, L. (2010). Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal, 4(2), 83-8. https://doi.org/10.1111/j.1752-699X.2009.00151.x
Tufvesson E, et al. Levels of Cysteinyl-leukotrienes in Exhaled Breath Condensate Are Not Due to Saliva Contamination. Clin Respir J. 2010;4(2):83-8. PubMed PMID: 20565481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. AU - Tufvesson,Ellen, AU - van Weele,Linda Jacqueline, AU - Ekedahl,Henrik, AU - Bjermer,Leif, PY - 2010/6/23/entrez PY - 2010/6/23/pubmed PY - 2010/9/29/medline SP - 83 EP - 8 JF - The clinical respiratory journal JO - Clin Respir J VL - 4 IS - 2 N2 - BACKGROUND AND AIMS: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. METHODS: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. RESULTS: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. CONCLUSION: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. SN - 1752-699X UR - https://www.unboundmedicine.com/medline/citation/20565481/Levels_of_cysteinyl_leukotrienes_in_exhaled_breath_condensate_are_not_due_to_saliva_contamination_ L2 - https://doi.org/10.1111/j.1752-699X.2009.00151.x DB - PRIME DP - Unbound Medicine ER -