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Effects of copper sulfate, hydrogen peroxide and N-phenyl-2-naphthylamine on oxidative stress and the expression of genes involved photosynthesis and microcystin disposition in Microcystis aeruginosa.
Aquat Toxicol. 2010 Sep 01; 99(3):405-12.AT

Abstract

Algal blooms have been increasing in prevalence all over the world, destroying ecosystems and placing other organisms at risk. Chemical remediation is one of most important methods of controlling algal bloom formation. The effects of copper sulfate, hydrogen peroxide (H(2)O(2)) and N-phenyl-2-naphthylamine on photosynthesis-related and microcystin-related gene transcription and physiological changes of Microcystis aeruginosa were analyzed. The results suggest that transcription of psaB, psbD1 and rbcL was inhibited by the three algaecides, which blocked the electron transport chain, significantly enhanced reactive oxygen species (ROS) accumulation and overwhelmed the antioxidant system. The increase in ROS destroyed pigment synthesis and membrane integrity, which inhibited or killed the algal cells. Furthermore, H(2)O(2) treatment down-regulated mcyD transcription, which indicated a decrease in the microcystin level in the cells. Our results demonstrate that H(2)O(2) has the greatest potential as an algaecide because it not only inhibits algae growth but may reduce microcystin synthesis.

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Authors+Show Affiliations

Qian H
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang, 310032, China.
Yu S
No affiliation info available
Sun Z
No affiliation info available
Xie X
No affiliation info available
Liu W
No affiliation info available
Fu Z
No affiliation info available

MeSH

2-NaphthylamineCatalaseCopper SulfateDisinfectantsGene ExpressionHarmful Algal BloomHydrogen PeroxideMalondialdehydeMicrocystinsMicrocystisOxidative StressPeroxidasePhotosynthesisPigments, BiologicalReactive Oxygen SpeciesSuperoxide Dismutase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20566224

Citation

Qian, Haifeng, et al. "Effects of Copper Sulfate, Hydrogen Peroxide and N-phenyl-2-naphthylamine On Oxidative Stress and the Expression of Genes Involved Photosynthesis and Microcystin Disposition in Microcystis Aeruginosa." Aquatic Toxicology (Amsterdam, Netherlands), vol. 99, no. 3, 2010, pp. 405-12.
Qian H, Yu S, Sun Z, et al. Effects of copper sulfate, hydrogen peroxide and N-phenyl-2-naphthylamine on oxidative stress and the expression of genes involved photosynthesis and microcystin disposition in Microcystis aeruginosa. Aquat Toxicol. 2010;99(3):405-12.
Qian, H., Yu, S., Sun, Z., Xie, X., Liu, W., & Fu, Z. (2010). Effects of copper sulfate, hydrogen peroxide and N-phenyl-2-naphthylamine on oxidative stress and the expression of genes involved photosynthesis and microcystin disposition in Microcystis aeruginosa. Aquatic Toxicology (Amsterdam, Netherlands), 99(3), 405-12. https://doi.org/10.1016/j.aquatox.2010.05.018
Qian H, et al. Effects of Copper Sulfate, Hydrogen Peroxide and N-phenyl-2-naphthylamine On Oxidative Stress and the Expression of Genes Involved Photosynthesis and Microcystin Disposition in Microcystis Aeruginosa. Aquat Toxicol. 2010 Sep 1;99(3):405-12. PubMed PMID: 20566224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of copper sulfate, hydrogen peroxide and N-phenyl-2-naphthylamine on oxidative stress and the expression of genes involved photosynthesis and microcystin disposition in Microcystis aeruginosa. AU - Qian,Haifeng, AU - Yu,Shuqiong, AU - Sun,Zhengqi, AU - Xie,Xiucai, AU - Liu,Weiping, AU - Fu,Zhengwei, Y1 - 2010/06/04/ PY - 2010/02/12/received PY - 2010/05/24/revised PY - 2010/05/29/accepted PY - 2010/6/23/entrez PY - 2010/6/23/pubmed PY - 2010/9/10/medline SP - 405 EP - 12 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 99 IS - 3 N2 - Algal blooms have been increasing in prevalence all over the world, destroying ecosystems and placing other organisms at risk. Chemical remediation is one of most important methods of controlling algal bloom formation. The effects of copper sulfate, hydrogen peroxide (H(2)O(2)) and N-phenyl-2-naphthylamine on photosynthesis-related and microcystin-related gene transcription and physiological changes of Microcystis aeruginosa were analyzed. The results suggest that transcription of psaB, psbD1 and rbcL was inhibited by the three algaecides, which blocked the electron transport chain, significantly enhanced reactive oxygen species (ROS) accumulation and overwhelmed the antioxidant system. The increase in ROS destroyed pigment synthesis and membrane integrity, which inhibited or killed the algal cells. Furthermore, H(2)O(2) treatment down-regulated mcyD transcription, which indicated a decrease in the microcystin level in the cells. Our results demonstrate that H(2)O(2) has the greatest potential as an algaecide because it not only inhibits algae growth but may reduce microcystin synthesis. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/20566224/Effects_of_copper_sulfate_hydrogen_peroxide_and_N_phenyl_2_naphthylamine_on_oxidative_stress_and_the_expression_of_genes_involved_photosynthesis_and_microcystin_disposition_in_Microcystis_aeruginosa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(10)00211-0 DB - PRIME DP - Unbound Medicine ER -