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Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail.
Mol Nutr Food Res 2010; 54(12):1744-52MN

Abstract

SCOPE

Atomic force microscopy (AFM) was used to directly measure the nanoscale adhesion forces between P-fimbriated Escherichia coli (E. coli) and human uroepithelial cells exposed to cranberry juice, in order to reveal the molecular mechanisms by which cranberry juice cocktail (CJC) affects bacterial adhesion.

METHODS AND RESULTS

Bacterial cell probes were created by attaching P-fimbriated E. coli HB101pDC1 or non-fimbriated E. coli HB101 to AFM tips, and the cellular probes were used to directly measure the adhesion forces between E. coli and uroepithelial cells in solutions containing: 0, 2.5, 5, 10, and 27 wt% CJC. Macroscale attachment of E. coli to uroepithelial cells was measured and correlated to nanoscale adhesion force measurements. The adhesion forces between E. coli HB101pDC1 and uroepithelial cells were dose-dependent, and decreased from 9.32±2.37 nN in the absence of CJC to 0.75±0.19 nN in 27 wt% CJC. Adhesion forces between E. coli HB101 and uroepithelial cells were low in buffer (0.74±0.18 nN), and did not change significantly in CJC (0.78±0.18 nN in 27 wt% CJC; P=0.794).

CONCLUSION

Our study shows that CJC significantly decreases nanoscale adhesion forces between P-fimbriated E. coli and uroepithelial cells.

Authors+Show Affiliations

Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20568234

Citation

Liu, Yatao, et al. "Direct Adhesion Force Measurements Between E. Coli and Human Uroepithelial Cells in Cranberry Juice Cocktail." Molecular Nutrition & Food Research, vol. 54, no. 12, 2010, pp. 1744-52.
Liu Y, Pinzón-Arango PA, Gallardo-Moreno AM, et al. Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail. Mol Nutr Food Res. 2010;54(12):1744-52.
Liu, Y., Pinzón-Arango, P. A., Gallardo-Moreno, A. M., & Camesano, T. A. (2010). Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail. Molecular Nutrition & Food Research, 54(12), pp. 1744-52. doi:10.1002/mnfr.200900535.
Liu Y, et al. Direct Adhesion Force Measurements Between E. Coli and Human Uroepithelial Cells in Cranberry Juice Cocktail. Mol Nutr Food Res. 2010;54(12):1744-52. PubMed PMID: 20568234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail. AU - Liu,Yatao, AU - Pinzón-Arango,Paola A, AU - Gallardo-Moreno,Amparo M, AU - Camesano,Terri A, PY - 2010/6/23/entrez PY - 2010/6/23/pubmed PY - 2011/6/1/medline SP - 1744 EP - 52 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 54 IS - 12 N2 - SCOPE: Atomic force microscopy (AFM) was used to directly measure the nanoscale adhesion forces between P-fimbriated Escherichia coli (E. coli) and human uroepithelial cells exposed to cranberry juice, in order to reveal the molecular mechanisms by which cranberry juice cocktail (CJC) affects bacterial adhesion. METHODS AND RESULTS: Bacterial cell probes were created by attaching P-fimbriated E. coli HB101pDC1 or non-fimbriated E. coli HB101 to AFM tips, and the cellular probes were used to directly measure the adhesion forces between E. coli and uroepithelial cells in solutions containing: 0, 2.5, 5, 10, and 27 wt% CJC. Macroscale attachment of E. coli to uroepithelial cells was measured and correlated to nanoscale adhesion force measurements. The adhesion forces between E. coli HB101pDC1 and uroepithelial cells were dose-dependent, and decreased from 9.32±2.37 nN in the absence of CJC to 0.75±0.19 nN in 27 wt% CJC. Adhesion forces between E. coli HB101 and uroepithelial cells were low in buffer (0.74±0.18 nN), and did not change significantly in CJC (0.78±0.18 nN in 27 wt% CJC; P=0.794). CONCLUSION: Our study shows that CJC significantly decreases nanoscale adhesion forces between P-fimbriated E. coli and uroepithelial cells. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/20568234/Direct_adhesion_force_measurements_between_E__coli_and_human_uroepithelial_cells_in_cranberry_juice_cocktail_ L2 - https://doi.org/10.1002/mnfr.200900535 DB - PRIME DP - Unbound Medicine ER -