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Role of Fosinopril and Valsartan on Klotho Gene Expression Induced by Angiotensin II in Rat Renal Tubular Epithelial Cells.
Kidney Blood Press Res. 2010; 33(3):186-92.KB

Abstract

BACKGROUND/AIMS

Klotho gene, a new anti-aging gene, is mainly expressed in the kidney tubules. Several studies have found the relationship between klotho and emergence and development of renal diseases. This study set out to explore the role of fosinopril (Fos) and valsartan (Val) on klotho expression induced by angiotensin II (Ang II) in rat renal tubular epithelial cells (NRK-52E).

METHODS

NRK-52E cells were divided into five groups according to the treatment of Ang II, Fos and Val. Transforming growth factor-β1 (TGF-β1), p38, phospho-p38 (p-p38), p53, and Sp1 protein expression were determined by immunohistochemical and Western blotting analysis. Klotho expression was detected by reverse transcription-polymerase chain reaction and Western blotting analysis.

RESULTS

Ang II upregulated TGF-β1, p-p38 and p53 expression, and inhibited Sp1 and klotho expression in NRK-52E cells. After the intervention of Fos and/or Val, TGF-β1, p-p38 and p53 expression were downregulated, Sp1 and klotho expression were upregulated. TGF-β1 and p53, Sp1 and klotho expression exhibited a positive linear correlation, respectively.

CONCLUSION

We conclude that Fos and Val have a protective role in Ang II-induced renal damage, and it may be through mechanism of inhibiting TGF-β1, p-p38 and p53 expression, thus upregulating Sp1 and klotho expression.

Authors+Show Affiliations

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20571281

Citation

Zhou, Q, et al. "Role of Fosinopril and Valsartan On Klotho Gene Expression Induced By Angiotensin II in Rat Renal Tubular Epithelial Cells." Kidney & Blood Pressure Research, vol. 33, no. 3, 2010, pp. 186-92.
Zhou Q, Lin S, Tang R, et al. Role of Fosinopril and Valsartan on Klotho Gene Expression Induced by Angiotensin II in Rat Renal Tubular Epithelial Cells. Kidney Blood Press Res. 2010;33(3):186-92.
Zhou, Q., Lin, S., Tang, R., Veeraragoo, P., Peng, W., & Wu, R. (2010). Role of Fosinopril and Valsartan on Klotho Gene Expression Induced by Angiotensin II in Rat Renal Tubular Epithelial Cells. Kidney & Blood Pressure Research, 33(3), 186-92. https://doi.org/10.1159/000316703
Zhou Q, et al. Role of Fosinopril and Valsartan On Klotho Gene Expression Induced By Angiotensin II in Rat Renal Tubular Epithelial Cells. Kidney Blood Press Res. 2010;33(3):186-92. PubMed PMID: 20571281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of Fosinopril and Valsartan on Klotho Gene Expression Induced by Angiotensin II in Rat Renal Tubular Epithelial Cells. AU - Zhou,Q, AU - Lin,S, AU - Tang,R, AU - Veeraragoo,P, AU - Peng,W, AU - Wu,R, Y1 - 2010/06/23/ PY - 2009/10/08/received PY - 2010/04/02/accepted PY - 2010/6/24/entrez PY - 2010/6/24/pubmed PY - 2015/10/20/medline SP - 186 EP - 92 JF - Kidney & blood pressure research JO - Kidney Blood Press Res VL - 33 IS - 3 N2 - BACKGROUND/AIMS: Klotho gene, a new anti-aging gene, is mainly expressed in the kidney tubules. Several studies have found the relationship between klotho and emergence and development of renal diseases. This study set out to explore the role of fosinopril (Fos) and valsartan (Val) on klotho expression induced by angiotensin II (Ang II) in rat renal tubular epithelial cells (NRK-52E). METHODS: NRK-52E cells were divided into five groups according to the treatment of Ang II, Fos and Val. Transforming growth factor-β1 (TGF-β1), p38, phospho-p38 (p-p38), p53, and Sp1 protein expression were determined by immunohistochemical and Western blotting analysis. Klotho expression was detected by reverse transcription-polymerase chain reaction and Western blotting analysis. RESULTS: Ang II upregulated TGF-β1, p-p38 and p53 expression, and inhibited Sp1 and klotho expression in NRK-52E cells. After the intervention of Fos and/or Val, TGF-β1, p-p38 and p53 expression were downregulated, Sp1 and klotho expression were upregulated. TGF-β1 and p53, Sp1 and klotho expression exhibited a positive linear correlation, respectively. CONCLUSION: We conclude that Fos and Val have a protective role in Ang II-induced renal damage, and it may be through mechanism of inhibiting TGF-β1, p-p38 and p53 expression, thus upregulating Sp1 and klotho expression. SN - 1423-0143 UR - https://www.unboundmedicine.com/medline/citation/20571281/Role_of_Fosinopril_and_Valsartan_on_Klotho_Gene_Expression_Induced_by_Angiotensin_II_in_Rat_Renal_Tubular_Epithelial_Cells_ L2 - https://www.karger.com?DOI=10.1159/000316703 DB - PRIME DP - Unbound Medicine ER -