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Multiple routes of hepatitis C virus transmission among injection drug users in Hai Phong, Northern Vietnam.
J Med Virol. 2010 Aug; 82(8):1355-63.JM

Abstract

To identify hepatitis C virus (HCV) transmission routes among injection drug users in Northern Vietnam, plasma samples were collected from 486 drug users in Hai Phong. Plasma viral RNA was extracted from 323 (66.5%) samples that were positive for anti-HCV antibodies. Portions of the HCV 5'-untranslated (5'UTR)-Core and NS5B genes were amplified by reverse-transcriptase polymerase chain reaction, sequenced directly, and genotyped in 194 and 195 specimens, respectively. Both regions were genotyped in 137 specimens. In the 5'UTR-Core region, genotype 6a was predominant (32.5%), followed by genotype 1a (23.7%), genotype 1b (20.6%), and genotype 6e (14.4%). In the NS5B region, genotype 1a was predominant (42.6%), followed by genotype 1b (24.1%), genotype 6a (14.4%), genotype 3b (7.2%), and genotype 6e (5.1%). Of the 137 specimens with both regions genotyped, 23 (16.8%) showed discordant genotyping results between the two regions, suggesting possible recombination and/or dual infection. Phylogenetic analysis revealed close associations between Hai Phong strains and strains from Southern China: the Yunnan province for genotype 3b; the Guangxi province for genotype 6e; the USA for genotype 1a; and Southern Vietnam for genotypes 1a and 6e. The human immunodeficiency virus (HIV) infection rate among HCV-infected injection drug users was 52.6-55.4% and did not differ significantly by HCV genotype. Most drug users infected with HIV-1 [98.8% (171/173)] were co-infected with HCV. These results suggest multiple routes of HCV transmission among injection drug users in Northern Vietnam that may also be HIV transmission routes.

Authors+Show Affiliations

Department of Viral Infection and International Health, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20572071

Citation

Tanimoto, Tomoaki, et al. "Multiple Routes of Hepatitis C Virus Transmission Among Injection Drug Users in Hai Phong, Northern Vietnam." Journal of Medical Virology, vol. 82, no. 8, 2010, pp. 1355-63.
Tanimoto T, Nguyen HC, Ishizaki A, et al. Multiple routes of hepatitis C virus transmission among injection drug users in Hai Phong, Northern Vietnam. J Med Virol. 2010;82(8):1355-63.
Tanimoto, T., Nguyen, H. C., Ishizaki, A., Chung, P. T., Hoang, T. T., Nguyen, V. T., Kageyama, S., Oka, S., Pham, V. T., & Ichimura, H. (2010). Multiple routes of hepatitis C virus transmission among injection drug users in Hai Phong, Northern Vietnam. Journal of Medical Virology, 82(8), 1355-63. https://doi.org/10.1002/jmv.21787
Tanimoto T, et al. Multiple Routes of Hepatitis C Virus Transmission Among Injection Drug Users in Hai Phong, Northern Vietnam. J Med Virol. 2010;82(8):1355-63. PubMed PMID: 20572071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple routes of hepatitis C virus transmission among injection drug users in Hai Phong, Northern Vietnam. AU - Tanimoto,Tomoaki, AU - Nguyen,Hung Cuong, AU - Ishizaki,Azumi, AU - Chung,Phan Thi Thu, AU - Hoang,Thi Thanh Huyen, AU - Nguyen,Vu Trung, AU - Kageyama,Seiji, AU - Oka,Shinichi, AU - Pham,Van Thuc, AU - Ichimura,Hiroshi, PY - 2010/6/24/entrez PY - 2010/6/24/pubmed PY - 2010/9/24/medline SP - 1355 EP - 63 JF - Journal of medical virology JO - J Med Virol VL - 82 IS - 8 N2 - To identify hepatitis C virus (HCV) transmission routes among injection drug users in Northern Vietnam, plasma samples were collected from 486 drug users in Hai Phong. Plasma viral RNA was extracted from 323 (66.5%) samples that were positive for anti-HCV antibodies. Portions of the HCV 5'-untranslated (5'UTR)-Core and NS5B genes were amplified by reverse-transcriptase polymerase chain reaction, sequenced directly, and genotyped in 194 and 195 specimens, respectively. Both regions were genotyped in 137 specimens. In the 5'UTR-Core region, genotype 6a was predominant (32.5%), followed by genotype 1a (23.7%), genotype 1b (20.6%), and genotype 6e (14.4%). In the NS5B region, genotype 1a was predominant (42.6%), followed by genotype 1b (24.1%), genotype 6a (14.4%), genotype 3b (7.2%), and genotype 6e (5.1%). Of the 137 specimens with both regions genotyped, 23 (16.8%) showed discordant genotyping results between the two regions, suggesting possible recombination and/or dual infection. Phylogenetic analysis revealed close associations between Hai Phong strains and strains from Southern China: the Yunnan province for genotype 3b; the Guangxi province for genotype 6e; the USA for genotype 1a; and Southern Vietnam for genotypes 1a and 6e. The human immunodeficiency virus (HIV) infection rate among HCV-infected injection drug users was 52.6-55.4% and did not differ significantly by HCV genotype. Most drug users infected with HIV-1 [98.8% (171/173)] were co-infected with HCV. These results suggest multiple routes of HCV transmission among injection drug users in Northern Vietnam that may also be HIV transmission routes. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/20572071/Multiple_routes_of_hepatitis_C_virus_transmission_among_injection_drug_users_in_Hai_Phong_Northern_Vietnam_ L2 - https://doi.org/10.1002/jmv.21787 DB - PRIME DP - Unbound Medicine ER -