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Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: an experimental study on rat myocardium, testicles and urinary bladder.
Food Chem Toxicol. 2010 Aug-Sep; 48(8-9):2326-36.FC

Abstract

The present study aimed to evaluate the role of DL-alpha-lipoic acid (LA) and squalene (SQ) on oxidative cardiac, testicular and urotoxic damage induced by cyclophosphamide (CP). Male Wistar rats were divided into four groups; three groups received a single intraperitoneal injection of CP (200mg/kg BW) to induce toxicity, and two of these groups received either LA (35 mg/kg BW) or SQ (0.4 ml/rat) orally 7 days before and 7 days after CP injection. A vehicle-treated control group was also included. Oxidative damage was observed by decreased serum total antioxidant capacity (TAC) level and abnormal alterations in glutathione peroxidase (GPx) and glutathione reductase (GR) activities, levels of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO) and calcium (Ca(+2)) in the heart, testes and urinary bladder of CP-administered rats. Cardiac marker enzyme activities; creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and aspartate transaminase (AST) showed severe declines whereas testicular markers; sorbitol dehydrogenase (SDH), gamma-glutamyl transferase (gamma-GT), acid and alkaline phosphatases (ACP and ALP), serum testosterone (T) level and haemoglobin (Hb) absorbance were abnormal. Histopathological observations were also altered. These CP-induced pathological alterations were attenuated by treatment with LA or SQ. These findings highlight the efficacy of LA and SQ as cytoprotectants in CP-induced toxicity.

Authors+Show Affiliations

Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20573578

Citation

Motawi, Tarek M K., et al. "Cytoprotective Effects of DL-alpha-lipoic Acid or Squalene On Cyclophosphamide-induced Oxidative Injury: an Experimental Study On Rat Myocardium, Testicles and Urinary Bladder." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 48, no. 8-9, 2010, pp. 2326-36.
Motawi TM, Sadik NA, Refaat A. Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: an experimental study on rat myocardium, testicles and urinary bladder. Food Chem Toxicol. 2010;48(8-9):2326-36.
Motawi, T. M., Sadik, N. A., & Refaat, A. (2010). Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: an experimental study on rat myocardium, testicles and urinary bladder. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 48(8-9), 2326-36. https://doi.org/10.1016/j.fct.2010.05.067
Motawi TM, Sadik NA, Refaat A. Cytoprotective Effects of DL-alpha-lipoic Acid or Squalene On Cyclophosphamide-induced Oxidative Injury: an Experimental Study On Rat Myocardium, Testicles and Urinary Bladder. Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2326-36. PubMed PMID: 20573578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytoprotective effects of DL-alpha-lipoic acid or squalene on cyclophosphamide-induced oxidative injury: an experimental study on rat myocardium, testicles and urinary bladder. AU - Motawi,Tarek M K, AU - Sadik,Nermin A H, AU - Refaat,Ayat, Y1 - 2010/05/31/ PY - 2010/03/09/received PY - 2010/05/09/revised PY - 2010/05/24/accepted PY - 2010/6/25/entrez PY - 2010/6/25/pubmed PY - 2010/11/4/medline SP - 2326 EP - 36 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 48 IS - 8-9 N2 - The present study aimed to evaluate the role of DL-alpha-lipoic acid (LA) and squalene (SQ) on oxidative cardiac, testicular and urotoxic damage induced by cyclophosphamide (CP). Male Wistar rats were divided into four groups; three groups received a single intraperitoneal injection of CP (200mg/kg BW) to induce toxicity, and two of these groups received either LA (35 mg/kg BW) or SQ (0.4 ml/rat) orally 7 days before and 7 days after CP injection. A vehicle-treated control group was also included. Oxidative damage was observed by decreased serum total antioxidant capacity (TAC) level and abnormal alterations in glutathione peroxidase (GPx) and glutathione reductase (GR) activities, levels of glutathione (GSH), malondialdehyde (MDA), nitric oxide (NO) and calcium (Ca(+2)) in the heart, testes and urinary bladder of CP-administered rats. Cardiac marker enzyme activities; creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and aspartate transaminase (AST) showed severe declines whereas testicular markers; sorbitol dehydrogenase (SDH), gamma-glutamyl transferase (gamma-GT), acid and alkaline phosphatases (ACP and ALP), serum testosterone (T) level and haemoglobin (Hb) absorbance were abnormal. Histopathological observations were also altered. These CP-induced pathological alterations were attenuated by treatment with LA or SQ. These findings highlight the efficacy of LA and SQ as cytoprotectants in CP-induced toxicity. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/20573578/Cytoprotective_effects_of_DL_alpha_lipoic_acid_or_squalene_on_cyclophosphamide_induced_oxidative_injury:_an_experimental_study_on_rat_myocardium_testicles_and_urinary_bladder_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(10)00360-1 DB - PRIME DP - Unbound Medicine ER -