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Burkholderia thailandensis whole cell antigen cross-reacts with B. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay.
Southeast Asian J Trop Med Public Health. 2010 Mar; 41(2):395-400.SA

Abstract

An immunofluorescent assay (IFAT) using whole cell antigen derived from Burkholderia thailandensis used for detection of total antibodies to Burkholderia pseudomallei, was found to compare favorably with a previous published report on a B. pseudomallei IFAT assay. At a 1:20 cut-off titer, the assay had high sensitivity (98.9%) and satisfactory specificity (92.3%), when tested against sera from 94 patients suspected of melioidosis. Sera from 12 patients with culture proven melioidosis gave absolute concordance with the 2 test antigens. No sera from 50 blood donors had a titer of > or =20. Cross-reactivity with patients' sera positive for Chlamydia, Mycoplasma, Legionella and typhoid was not observed, except for 3 sera from typhus patients and one from a patient with leptospirosis. The major advantage of this assay is that the cultivation and preparation of B. thailandensis as antigen can be carried out in any laboratory with basic microbiological set-up. The serodiagnosis of melioidosis can be made safe for medical laboratory personnel, particularly in B. pseudomallei endemic regions.

Authors+Show Affiliations

Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. puthu@um.edu.myNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20578523

Citation

Puthucheary, S D., et al. "Burkholderia Thailandensis Whole Cell Antigen Cross-reacts With B. Pseudomallei Antibodies From Patients With Melioidosis in an Immunofluorescent Assay." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 41, no. 2, 2010, pp. 395-400.
Puthucheary SD, Anuar AS, Tee TS. Burkholderia thailandensis whole cell antigen cross-reacts with B. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay. Southeast Asian J Trop Med Public Health. 2010;41(2):395-400.
Puthucheary, S. D., Anuar, A. S., & Tee, T. S. (2010). Burkholderia thailandensis whole cell antigen cross-reacts with B. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay. The Southeast Asian Journal of Tropical Medicine and Public Health, 41(2), 395-400.
Puthucheary SD, Anuar AS, Tee TS. Burkholderia Thailandensis Whole Cell Antigen Cross-reacts With B. Pseudomallei Antibodies From Patients With Melioidosis in an Immunofluorescent Assay. Southeast Asian J Trop Med Public Health. 2010;41(2):395-400. PubMed PMID: 20578523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia thailandensis whole cell antigen cross-reacts with B. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay. AU - Puthucheary,S D, AU - Anuar,Amal S S, AU - Tee,Tay Sun, PY - 2010/6/29/entrez PY - 2010/6/29/pubmed PY - 2010/10/12/medline SP - 395 EP - 400 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 41 IS - 2 N2 - An immunofluorescent assay (IFAT) using whole cell antigen derived from Burkholderia thailandensis used for detection of total antibodies to Burkholderia pseudomallei, was found to compare favorably with a previous published report on a B. pseudomallei IFAT assay. At a 1:20 cut-off titer, the assay had high sensitivity (98.9%) and satisfactory specificity (92.3%), when tested against sera from 94 patients suspected of melioidosis. Sera from 12 patients with culture proven melioidosis gave absolute concordance with the 2 test antigens. No sera from 50 blood donors had a titer of > or =20. Cross-reactivity with patients' sera positive for Chlamydia, Mycoplasma, Legionella and typhoid was not observed, except for 3 sera from typhus patients and one from a patient with leptospirosis. The major advantage of this assay is that the cultivation and preparation of B. thailandensis as antigen can be carried out in any laboratory with basic microbiological set-up. The serodiagnosis of melioidosis can be made safe for medical laboratory personnel, particularly in B. pseudomallei endemic regions. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/20578523/Burkholderia_thailandensis_whole_cell_antigen_cross_reacts_with_B__pseudomallei_antibodies_from_patients_with_melioidosis_in_an_immunofluorescent_assay_ DB - PRIME DP - Unbound Medicine ER -