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Iridoid extracts from Ajuga iva increase the antioxidant enzyme activities in red blood cells of rats fed a cholesterol-rich diet.
Nutr Res. 2010 May; 30(5):358-65.NR

Abstract

The lyophilized aqueous extract of Ajuga iva (Ai) is able to reduce oxidative stress, which may prevent lipid peroxidation in hypercholesterolemic rats. Iridoids (I) were isolated from Ai. We hypothesized that the antioxidant defense status in red blood cells (RBC) and tissues in rats fed a cholesterol-rich diet and treated with Ai may be correlated to these compounds. Male Wistar rats (n = 32) weighing 120 +/- 5 g were fed a diet containing 1% cholesterol for 15 days. After this phase, hypercholesterolemic (HC) rats were divided into groups, fed the same diet, and received either the same or different doses (5, 10, or 15 mg/kg body weight by intraperitoneal injection) of I for 15 days. Compared with the HC group, total cholesterol value was 1.4- and 1.2-fold lower in the I(5)-HC and I(10)-HC groups. Serum thiobarbituric acid reactive substance content was 2.3-, 2.9-, and 3-fold lower in the I(5)-HC, I(10)-HC, and I(15)-HC groups compared with the HC group. In RBC, glutathione peroxidase, glutathione reductase, and superoxide dismutase activities were significantly higher in the I(5)-HC, I(10)-HC, and I(15)-HC groups than the HC group. Liver, heart, and muscle glutathione peroxidase and superoxide dismutase activities were significantly higher in the groups treated with I than the HC group. Muscle glutathione reductase activity was increased 1.4-fold in the I(5)-HC, 1.5-fold in the I(10)-HC, and 1.5-fold in the I(15)-HC group. In HC rats, different doses of I increase the antioxidant enzyme activities in RBC and act differently in tissues. Treatment with I may play an important role in suppressing oxidative stress caused by dietary cholesterol and, thus, may be useful for the prevention and/or early treatment of hypercholesterolemia.

Authors+Show Affiliations

Laboratoire de Nutrition Clinique et Métabolique, Département de Biologie, Faculté des Sciences, Université d'Oran Es-Sénia, 31000 Oran, Algeria. bsherazede@yahoo.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20579528

Citation

Bouderbala, Sherazede, et al. "Iridoid Extracts From Ajuga Iva Increase the Antioxidant Enzyme Activities in Red Blood Cells of Rats Fed a Cholesterol-rich Diet." Nutrition Research (New York, N.Y.), vol. 30, no. 5, 2010, pp. 358-65.
Bouderbala S, Prost J, Lacaille-Dubois MA, et al. Iridoid extracts from Ajuga iva increase the antioxidant enzyme activities in red blood cells of rats fed a cholesterol-rich diet. Nutr Res. 2010;30(5):358-65.
Bouderbala, S., Prost, J., Lacaille-Dubois, M. A., & Bouchenak, M. (2010). Iridoid extracts from Ajuga iva increase the antioxidant enzyme activities in red blood cells of rats fed a cholesterol-rich diet. Nutrition Research (New York, N.Y.), 30(5), 358-65. https://doi.org/10.1016/j.nutres.2010.05.004
Bouderbala S, et al. Iridoid Extracts From Ajuga Iva Increase the Antioxidant Enzyme Activities in Red Blood Cells of Rats Fed a Cholesterol-rich Diet. Nutr Res. 2010;30(5):358-65. PubMed PMID: 20579528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iridoid extracts from Ajuga iva increase the antioxidant enzyme activities in red blood cells of rats fed a cholesterol-rich diet. AU - Bouderbala,Sherazede, AU - Prost,Josiane, AU - Lacaille-Dubois,Marie Aleth, AU - Bouchenak,Malika, PY - 2010/03/11/received PY - 2010/04/23/revised PY - 2010/05/05/accepted PY - 2010/6/29/entrez PY - 2010/6/29/pubmed PY - 2010/10/27/medline SP - 358 EP - 65 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 30 IS - 5 N2 - The lyophilized aqueous extract of Ajuga iva (Ai) is able to reduce oxidative stress, which may prevent lipid peroxidation in hypercholesterolemic rats. Iridoids (I) were isolated from Ai. We hypothesized that the antioxidant defense status in red blood cells (RBC) and tissues in rats fed a cholesterol-rich diet and treated with Ai may be correlated to these compounds. Male Wistar rats (n = 32) weighing 120 +/- 5 g were fed a diet containing 1% cholesterol for 15 days. After this phase, hypercholesterolemic (HC) rats were divided into groups, fed the same diet, and received either the same or different doses (5, 10, or 15 mg/kg body weight by intraperitoneal injection) of I for 15 days. Compared with the HC group, total cholesterol value was 1.4- and 1.2-fold lower in the I(5)-HC and I(10)-HC groups. Serum thiobarbituric acid reactive substance content was 2.3-, 2.9-, and 3-fold lower in the I(5)-HC, I(10)-HC, and I(15)-HC groups compared with the HC group. In RBC, glutathione peroxidase, glutathione reductase, and superoxide dismutase activities were significantly higher in the I(5)-HC, I(10)-HC, and I(15)-HC groups than the HC group. Liver, heart, and muscle glutathione peroxidase and superoxide dismutase activities were significantly higher in the groups treated with I than the HC group. Muscle glutathione reductase activity was increased 1.4-fold in the I(5)-HC, 1.5-fold in the I(10)-HC, and 1.5-fold in the I(15)-HC group. In HC rats, different doses of I increase the antioxidant enzyme activities in RBC and act differently in tissues. Treatment with I may play an important role in suppressing oxidative stress caused by dietary cholesterol and, thus, may be useful for the prevention and/or early treatment of hypercholesterolemia. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/20579528/Iridoid_extracts_from_Ajuga_iva_increase_the_antioxidant_enzyme_activities_in_red_blood_cells_of_rats_fed_a_cholesterol_rich_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(10)00078-3 DB - PRIME DP - Unbound Medicine ER -