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Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men.
J Physiol. 2010 Aug 15; 588(Pt 16):3119-30.JP

Abstract

We aimed to determine if any mechanistic differences exist between a single set (1SET) and multiple sets (i.e. 3 sets; 3SET) of resistance exercise by utilizing a primed constant infusion of [ring-13C6]phenylalanine to determine myofibrillar protein synthesis (MPS) and Western blot analysis to examine anabolic signalling molecule phosphorylation following an acute bout of resistance exercise. Eight resistance-trained men (24+/-5 years, BMI=25+/-4 kg m2) were randomly assigned to perform unilateral leg extension exercise at 70% concentric one repetition maximum (1RM) until volitional fatigue for 1SET or 3SET. Biopsies from the vastus lateralis were taken in the fasted state (Fast) and fed state (Fed; 20 g of whey protein isolate) at rest, 5 h Fed, 24 h Fast and 29 h Fed post-exercise. Fed-state MPS was transiently elevated above rest at 5 h for 1SET (2.3-fold) and returned to resting levels by 29 h post-exercise. However, the exercise induced increase in MPS following 3SET was superior in amplitude and duration as compared to 1SET at both 5 h (3.1-fold above rest) and 29 h post-exercise (2.3-fold above rest). Phosphorylation of 70 kDa S6 protein kinase (p70S6K) demonstrated a coordinated increase with MPS at 5 h and 29 h post-exercise such that the extent of p70S6K phosphorylation was related to the MPS response (r=0.338, P=0.033). Phosphorylation of 90 kDa ribosomal S6 protein kinase (p90RSK) and ribosomal protein S6 (rps6) was similar for 1SET and 3SET at 24 h Fast and 29 h Fed, respectively. However, 3SET induced a greater activation of eukaryotic translation initiation factor 2B (eIF2B) and rpS6 at 5 h Fed. These data suggest that 3SET of resistance exercise is more anabolic than 1SET and may lead to greater increases in myofibrillar protein accretion over time.

Authors+Show Affiliations

Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20581041

Citation

Burd, Nicholas A., et al. "Resistance Exercise Volume Affects Myofibrillar Protein Synthesis and Anabolic Signalling Molecule Phosphorylation in Young Men." The Journal of Physiology, vol. 588, no. Pt 16, 2010, pp. 3119-30.
Burd NA, Holwerda AM, Selby KC, et al. Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. J Physiol. 2010;588(Pt 16):3119-30.
Burd, N. A., Holwerda, A. M., Selby, K. C., West, D. W., Staples, A. W., Cain, N. E., Cashaback, J. G., Potvin, J. R., Baker, S. K., & Phillips, S. M. (2010). Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. The Journal of Physiology, 588(Pt 16), 3119-30. https://doi.org/10.1113/jphysiol.2010.192856
Burd NA, et al. Resistance Exercise Volume Affects Myofibrillar Protein Synthesis and Anabolic Signalling Molecule Phosphorylation in Young Men. J Physiol. 2010 Aug 15;588(Pt 16):3119-30. PubMed PMID: 20581041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men. AU - Burd,Nicholas A, AU - Holwerda,Andrew M, AU - Selby,Keegan C, AU - West,Daniel W D, AU - Staples,Aaron W, AU - Cain,Nathan E, AU - Cashaback,Joshua G A, AU - Potvin,James R, AU - Baker,Steven K, AU - Phillips,Stuart M, Y1 - 2010/06/25/ PY - 2010/6/29/entrez PY - 2010/6/29/pubmed PY - 2010/12/14/medline SP - 3119 EP - 30 JF - The Journal of physiology JO - J Physiol VL - 588 IS - Pt 16 N2 - We aimed to determine if any mechanistic differences exist between a single set (1SET) and multiple sets (i.e. 3 sets; 3SET) of resistance exercise by utilizing a primed constant infusion of [ring-13C6]phenylalanine to determine myofibrillar protein synthesis (MPS) and Western blot analysis to examine anabolic signalling molecule phosphorylation following an acute bout of resistance exercise. Eight resistance-trained men (24+/-5 years, BMI=25+/-4 kg m2) were randomly assigned to perform unilateral leg extension exercise at 70% concentric one repetition maximum (1RM) until volitional fatigue for 1SET or 3SET. Biopsies from the vastus lateralis were taken in the fasted state (Fast) and fed state (Fed; 20 g of whey protein isolate) at rest, 5 h Fed, 24 h Fast and 29 h Fed post-exercise. Fed-state MPS was transiently elevated above rest at 5 h for 1SET (2.3-fold) and returned to resting levels by 29 h post-exercise. However, the exercise induced increase in MPS following 3SET was superior in amplitude and duration as compared to 1SET at both 5 h (3.1-fold above rest) and 29 h post-exercise (2.3-fold above rest). Phosphorylation of 70 kDa S6 protein kinase (p70S6K) demonstrated a coordinated increase with MPS at 5 h and 29 h post-exercise such that the extent of p70S6K phosphorylation was related to the MPS response (r=0.338, P=0.033). Phosphorylation of 90 kDa ribosomal S6 protein kinase (p90RSK) and ribosomal protein S6 (rps6) was similar for 1SET and 3SET at 24 h Fast and 29 h Fed, respectively. However, 3SET induced a greater activation of eukaryotic translation initiation factor 2B (eIF2B) and rpS6 at 5 h Fed. These data suggest that 3SET of resistance exercise is more anabolic than 1SET and may lead to greater increases in myofibrillar protein accretion over time. SN - 1469-7793 UR - https://www.unboundmedicine.com/medline/citation/20581041/Resistance_exercise_volume_affects_myofibrillar_protein_synthesis_and_anabolic_signalling_molecule_phosphorylation_in_young_men_ L2 - https://doi.org/10.1113/jphysiol.2010.192856 DB - PRIME DP - Unbound Medicine ER -