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Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC.
J Bacteriol. 2010 Sep; 192(17):4498-503.JB

Abstract

Tripartite efflux pumps found in Gram-negative bacteria are involved in antibiotic resistance and toxic-protein secretion. In this study, we show, using site-directed mutational analyses, that the conserved residues located in the tip region of the alpha-hairpin of the membrane fusion protein (MFP) AcrA play an essential role in the action of the tripartite efflux pump AcrAB-TolC. In addition, we provide in vivo functional data showing that both the length and the amino acid sequence of the alpha-hairpin of AcrA can be flexible for the formation of a functional AcrAB-TolC pump. Genetic-complementation experiments further indicated functional interrelationships between the AcrA hairpin tip region and the TolC aperture tip region. Our findings may offer a molecular basis for understanding the multidrug resistance of pathogenic bacteria.

Authors+Show Affiliations

Department of Life Science (BK21 Program), Chung-Ang University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20581201

Citation

Kim, Hong-Man, et al. "Functional Relationships Between the AcrA Hairpin Tip Region and the TolC Aperture Tip Region for the Formation of the Bacterial Tripartite Efflux Pump AcrAB-TolC." Journal of Bacteriology, vol. 192, no. 17, 2010, pp. 4498-503.
Kim HM, Xu Y, Lee M, et al. Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC. J Bacteriol. 2010;192(17):4498-503.
Kim, H. M., Xu, Y., Lee, M., Piao, S., Sim, S. H., Ha, N. C., & Lee, K. (2010). Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC. Journal of Bacteriology, 192(17), 4498-503. https://doi.org/10.1128/JB.00334-10
Kim HM, et al. Functional Relationships Between the AcrA Hairpin Tip Region and the TolC Aperture Tip Region for the Formation of the Bacterial Tripartite Efflux Pump AcrAB-TolC. J Bacteriol. 2010;192(17):4498-503. PubMed PMID: 20581201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC. AU - Kim,Hong-Man, AU - Xu,Yongbin, AU - Lee,Minho, AU - Piao,Shunfu, AU - Sim,Se-Hoon, AU - Ha,Nam-Chul, AU - Lee,Kangseok, Y1 - 2010/06/25/ PY - 2010/6/29/entrez PY - 2010/6/29/pubmed PY - 2010/9/14/medline SP - 4498 EP - 503 JF - Journal of bacteriology JO - J. Bacteriol. VL - 192 IS - 17 N2 - Tripartite efflux pumps found in Gram-negative bacteria are involved in antibiotic resistance and toxic-protein secretion. In this study, we show, using site-directed mutational analyses, that the conserved residues located in the tip region of the alpha-hairpin of the membrane fusion protein (MFP) AcrA play an essential role in the action of the tripartite efflux pump AcrAB-TolC. In addition, we provide in vivo functional data showing that both the length and the amino acid sequence of the alpha-hairpin of AcrA can be flexible for the formation of a functional AcrAB-TolC pump. Genetic-complementation experiments further indicated functional interrelationships between the AcrA hairpin tip region and the TolC aperture tip region. Our findings may offer a molecular basis for understanding the multidrug resistance of pathogenic bacteria. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/20581201/Functional_relationships_between_the_AcrA_hairpin_tip_region_and_the_TolC_aperture_tip_region_for_the_formation_of_the_bacterial_tripartite_efflux_pump_AcrAB_TolC_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=20581201 DB - PRIME DP - Unbound Medicine ER -