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[CAP59 gene amplification in Cryptococcus neoformans and Cryptococcus gattii directly from a yeast suspension].
Rev Argent Microbiol. 2010 Apr-Jun; 42(2):91-4.RA

Abstract

Cryptococcus is an encapsulated yeast of class Basidiomycetes, etiologic agent of cryptococcosis. Cryptococcosis is one of the most common opportunistic infections in immunosuppressed patients, although it can affect immunocompetent individuals. In recent years, the identification of medically important fungal species has been achieved through the amplification of specific regions or genes of fungal DNA by polymerase chain reaction (PCR). The CAP59 gene is involved in the synthesis of the capsule in Cryptococcus neoformans and Cryptococcus gattii, and is useful in the molecular identification of serotypes. In this research, we use yeasts of different serotypes from collection strains and C. neoformans isolates recovered from patients with cryptococcosis. A standardized yeast suspension from different Cryptococcus isolates as template allowed CAP59 gene amplification. This procedure was quick, simple, and inexpensive and required no PCR steps. This is important for taxonomic studies in laboratories with implemented molecular biology tools.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Siachoque N
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Jewtuchowicz VM
No affiliation info available
Iovannitti C
No affiliation info available
Mujica MT
No affiliation info available

MeSH

CryptococcosisCryptococcus gattiiCryptococcus neoformansDNA, FungalFungal ProteinsHumansMycologyNucleic Acid Amplification TechniquesPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSensitivity and SpecificitySerotypingSuspensions

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

20589327

Citation

Siachoque, N, et al. "[CAP59 Gene Amplification in Cryptococcus Neoformans and Cryptococcus Gattii Directly From a Yeast Suspension]." Revista Argentina De Microbiologia, vol. 42, no. 2, 2010, pp. 91-4.
Siachoque N, Jewtuchowicz VM, Iovannitti C, et al. [CAP59 gene amplification in Cryptococcus neoformans and Cryptococcus gattii directly from a yeast suspension]. Rev Argent Microbiol. 2010;42(2):91-4.
Siachoque, N., Jewtuchowicz, V. M., Iovannitti, C., & Mujica, M. T. (2010). [CAP59 gene amplification in Cryptococcus neoformans and Cryptococcus gattii directly from a yeast suspension]. Revista Argentina De Microbiologia, 42(2), 91-4. https://doi.org/10.1590/S0325-75412010000200004
Siachoque N, et al. [CAP59 Gene Amplification in Cryptococcus Neoformans and Cryptococcus Gattii Directly From a Yeast Suspension]. Rev Argent Microbiol. 2010 Apr-Jun;42(2):91-4. PubMed PMID: 20589327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [CAP59 gene amplification in Cryptococcus neoformans and Cryptococcus gattii directly from a yeast suspension]. AU - Siachoque,N, AU - Jewtuchowicz,V M, AU - Iovannitti,C, AU - Mujica,M T, PY - 2009/09/08/received PY - 2010/01/12/accepted PY - 2010/7/1/entrez PY - 2010/7/1/pubmed PY - 2010/10/6/medline SP - 91 EP - 4 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 42 IS - 2 N2 - Cryptococcus is an encapsulated yeast of class Basidiomycetes, etiologic agent of cryptococcosis. Cryptococcosis is one of the most common opportunistic infections in immunosuppressed patients, although it can affect immunocompetent individuals. In recent years, the identification of medically important fungal species has been achieved through the amplification of specific regions or genes of fungal DNA by polymerase chain reaction (PCR). The CAP59 gene is involved in the synthesis of the capsule in Cryptococcus neoformans and Cryptococcus gattii, and is useful in the molecular identification of serotypes. In this research, we use yeasts of different serotypes from collection strains and C. neoformans isolates recovered from patients with cryptococcosis. A standardized yeast suspension from different Cryptococcus isolates as template allowed CAP59 gene amplification. This procedure was quick, simple, and inexpensive and required no PCR steps. This is important for taxonomic studies in laboratories with implemented molecular biology tools. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/20589327/[CAP59_gene_amplification_in_Cryptococcus_neoformans_and_Cryptococcus_gattii_directly_from_a_yeast_suspension]_ DB - PRIME DP - Unbound Medicine ER -