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Developmental ontogeny of autoantigens associated with localized autoimmunity in murine testis and epididymis.
J Reprod Immunol. 2010 Dec; 87(1-2):45-51.JR

Abstract

Experimental autoimmune orchitis (EAO) with experimental autoimmune epididymitis (EAE) can be induced in mice by immunization with testicular antigens emulsified in adjuvants. On immunization with syngeneic testicular germ cells (TGC) alone, EAO with no EAE is induced in mice. Recently, we found that EAE with no EAO can be induced in vasectomized mice by immunization with TGC. In the present study, we investigated the appearance of autoantigens relevant to EAO and EAE by reacting each immune serum sample with testes and epididymides extracts from normal mice of various ages by immunoblotting. The results showed that the antisera obtained from mice with EAO lesions specifically defined testicular antigens with molecular weights of 15 kDa, 40 kDa, 75 kDa and >200 kDa from 4 weeks of age, but the antisera obtained from mice with EAE strongly defined both testicular and epididymal antigens of 25 kDa from 5 and 8 weeks of ages, respectively. These results suggest that vasectomy changes the target autoantigens in TGC-induced autoimmunity.

Authors+Show Affiliations

Department of Anatomy, Tokyo Medical University, Shinjuku 6-1-1, Shinjuku-ku, Shinjuku, Tokyo 160-8402, Japan. quning@tokyo-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20591496

Citation

Qu, Ning, et al. "Developmental Ontogeny of Autoantigens Associated With Localized Autoimmunity in Murine Testis and Epididymis." Journal of Reproductive Immunology, vol. 87, no. 1-2, 2010, pp. 45-51.
Qu N, Naito M, Terayama H, et al. Developmental ontogeny of autoantigens associated with localized autoimmunity in murine testis and epididymis. J Reprod Immunol. 2010;87(1-2):45-51.
Qu, N., Naito, M., Terayama, H., Hirai, S., Li, J., Ogawa, Y., Kitaoka, M., & Itoh, M. (2010). Developmental ontogeny of autoantigens associated with localized autoimmunity in murine testis and epididymis. Journal of Reproductive Immunology, 87(1-2), 45-51. https://doi.org/10.1016/j.jri.2010.05.005
Qu N, et al. Developmental Ontogeny of Autoantigens Associated With Localized Autoimmunity in Murine Testis and Epididymis. J Reprod Immunol. 2010;87(1-2):45-51. PubMed PMID: 20591496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental ontogeny of autoantigens associated with localized autoimmunity in murine testis and epididymis. AU - Qu,Ning, AU - Naito,Munekazu, AU - Terayama,Hayato, AU - Hirai,Shuichi, AU - Li,Jun, AU - Ogawa,Yuki, AU - Kitaoka,Miyuki, AU - Itoh,Masahiro, Y1 - 2010/06/29/ PY - 2010/02/26/received PY - 2010/04/28/revised PY - 2010/05/24/accepted PY - 2010/7/2/entrez PY - 2010/7/2/pubmed PY - 2011/5/21/medline SP - 45 EP - 51 JF - Journal of reproductive immunology JO - J Reprod Immunol VL - 87 IS - 1-2 N2 - Experimental autoimmune orchitis (EAO) with experimental autoimmune epididymitis (EAE) can be induced in mice by immunization with testicular antigens emulsified in adjuvants. On immunization with syngeneic testicular germ cells (TGC) alone, EAO with no EAE is induced in mice. Recently, we found that EAE with no EAO can be induced in vasectomized mice by immunization with TGC. In the present study, we investigated the appearance of autoantigens relevant to EAO and EAE by reacting each immune serum sample with testes and epididymides extracts from normal mice of various ages by immunoblotting. The results showed that the antisera obtained from mice with EAO lesions specifically defined testicular antigens with molecular weights of 15 kDa, 40 kDa, 75 kDa and >200 kDa from 4 weeks of age, but the antisera obtained from mice with EAE strongly defined both testicular and epididymal antigens of 25 kDa from 5 and 8 weeks of ages, respectively. These results suggest that vasectomy changes the target autoantigens in TGC-induced autoimmunity. SN - 1872-7603 UR - https://www.unboundmedicine.com/medline/citation/20591496/Developmental_ontogeny_of_autoantigens_associated_with_localized_autoimmunity_in_murine_testis_and_epididymis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(10)00100-2 DB - PRIME DP - Unbound Medicine ER -