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Hypersensitive response-like reaction is associated with hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii coss.
PLoS One. 2010 Jun 25; 5(6):e11326.Plos

Abstract

BACKGROUND

Hybrid speciation is classified into homoploid and polyploid based on ploidy level. Common wheat is an allohexaploid species that originated from a naturally occurring interploidy cross between tetraploid wheat and diploid wild wheat Aegilops tauschii Coss. Aegilops tauschii provides wide naturally occurring genetic variation. Sometimes its triploid hybrids with tetraploid wheat show the following four types of hybrid growth abnormalities: types II and III hybrid necrosis, hybrid chlorosis, and severe growth abortion. The growth abnormalities in the triploid hybrids could act as postzygotic hybridization barriers to prevent formation of hexaploid wheat.

METHODOLOGY/PRINCIPAL FINDINGS

Here, we report on the geographical and phylogenetic distribution of Ae. tauschii accessions inducing the hybrid growth abnormalities and showed that they are widely distributed across growth habitats in Ae. tauschii. Molecular and cytological characterization of the type III necrosis phenotype was performed. The hybrid abnormality causing accessions were widely distributed across growth habitats in Ae. tauschii. Transcriptome analysis showed that a number of defense-related genes such as pathogenesis-related genes were highly up-regulated in the type III necrosis lines. Transmission electron microscope observation revealed that cell death occurred accompanied by generation of reactive oxygen species in leaves undergoing type III necrosis. The reduction of photosynthetic activity occurred prior to the appearance of necrotic symptoms on the leaves exhibiting hybrid necrosis.

CONCLUSIONS/SIGNIFICANCE

Taking these results together strongly suggests that an autoimmune response might be triggered by intergenomic incompatibility between the tetraploid wheat and Ae. tauschii genomes in type III necrosis, and that genetically programmed cell death could be regarded as a hypersensitive response-like cell death similar to that observed in Arabidopsis intraspecific and Nicotiana interspecific hybrids. Only Ae. tauschii accessions without such inhibiting factors could be candidates for the D-genome donor for the present hexaploid wheat.

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Authors+Show Affiliations

Mizuno N
Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Hosogi N
No affiliation info available
Park P
No affiliation info available
Takumi S
No affiliation info available

MeSH

Genes, PlantHybridization, GeneticNecrosisPhotosynthesisPolyploidyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20593003

Citation

Mizuno, Nobuyuki, et al. "Hypersensitive Response-like Reaction Is Associated With Hybrid Necrosis in Interspecific Crosses Between Tetraploid Wheat and Aegilops Tauschii Coss." PloS One, vol. 5, no. 6, 2010, pp. e11326.
Mizuno N, Hosogi N, Park P, et al. Hypersensitive response-like reaction is associated with hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii coss. PLoS One. 2010;5(6):e11326.
Mizuno, N., Hosogi, N., Park, P., & Takumi, S. (2010). Hypersensitive response-like reaction is associated with hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii coss. PloS One, 5(6), e11326. https://doi.org/10.1371/journal.pone.0011326
Mizuno N, et al. Hypersensitive Response-like Reaction Is Associated With Hybrid Necrosis in Interspecific Crosses Between Tetraploid Wheat and Aegilops Tauschii Coss. PLoS One. 2010 Jun 25;5(6):e11326. PubMed PMID: 20593003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypersensitive response-like reaction is associated with hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii coss. AU - Mizuno,Nobuyuki, AU - Hosogi,Naoki, AU - Park,Pyoyun, AU - Takumi,Shigeo, Y1 - 2010/06/25/ PY - 2010/02/04/received PY - 2010/06/04/accepted PY - 2010/7/2/entrez PY - 2010/7/2/pubmed PY - 2010/9/2/medline SP - e11326 EP - e11326 JF - PloS one JO - PLoS One VL - 5 IS - 6 N2 - BACKGROUND: Hybrid speciation is classified into homoploid and polyploid based on ploidy level. Common wheat is an allohexaploid species that originated from a naturally occurring interploidy cross between tetraploid wheat and diploid wild wheat Aegilops tauschii Coss. Aegilops tauschii provides wide naturally occurring genetic variation. Sometimes its triploid hybrids with tetraploid wheat show the following four types of hybrid growth abnormalities: types II and III hybrid necrosis, hybrid chlorosis, and severe growth abortion. The growth abnormalities in the triploid hybrids could act as postzygotic hybridization barriers to prevent formation of hexaploid wheat. METHODOLOGY/PRINCIPAL FINDINGS: Here, we report on the geographical and phylogenetic distribution of Ae. tauschii accessions inducing the hybrid growth abnormalities and showed that they are widely distributed across growth habitats in Ae. tauschii. Molecular and cytological characterization of the type III necrosis phenotype was performed. The hybrid abnormality causing accessions were widely distributed across growth habitats in Ae. tauschii. Transcriptome analysis showed that a number of defense-related genes such as pathogenesis-related genes were highly up-regulated in the type III necrosis lines. Transmission electron microscope observation revealed that cell death occurred accompanied by generation of reactive oxygen species in leaves undergoing type III necrosis. The reduction of photosynthetic activity occurred prior to the appearance of necrotic symptoms on the leaves exhibiting hybrid necrosis. CONCLUSIONS/SIGNIFICANCE: Taking these results together strongly suggests that an autoimmune response might be triggered by intergenomic incompatibility between the tetraploid wheat and Ae. tauschii genomes in type III necrosis, and that genetically programmed cell death could be regarded as a hypersensitive response-like cell death similar to that observed in Arabidopsis intraspecific and Nicotiana interspecific hybrids. Only Ae. tauschii accessions without such inhibiting factors could be candidates for the D-genome donor for the present hexaploid wheat. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/20593003/Hypersensitive_response_like_reaction_is_associated_with_hybrid_necrosis_in_interspecific_crosses_between_tetraploid_wheat_and_Aegilops_tauschii_coss_ L2 - https://dx.plos.org/10.1371/journal.pone.0011326 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓27 ↑30]

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