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Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis.
J Comp Neurol. 2010 Sep 01; 518(17):3557-69.JC

Abstract

Trigeminal afferents convey nociceptive information from the corneal surface of the eye to the trigeminal subnucleus caudalis (Vc). Trigeminal afferents, like other nociceptors, are thought to use glutamate and neuropeptides as neurotransmitters. The current studies examined whether corneal afferents contain both neuropeptides and vesicular glutamate transporters. Corneal afferents to the Vc were identified by using cholera toxin B (CTb). Corneal afferents project in two clusters to the rostral and caudal borders of the Vc, regions that contain functionally distinct nociceptive neurons. Thus, corneal afferents projecting to these two regions were examined separately. Dual immunocytochemical studies combined CTb with either calcitonin gene-related peptide (CGRP), substance P (SP), vesicular glutamate transporter 1 (VGluT1), or VGluT2. Corneal afferents were more likely to contain CGRP than SP, and corneal afferents projecting to the rostral region were more likely to contain CGRP than afferents projecting caudally. Overall, corneal afferents were equally likely to contain VGluT1 or VGluT2. Together, 61% of corneal afferents contained either VGluT1 or VGluT2, suggesting that some afferents lack a VGluT. Caudal corneal afferents were more likely to contain VGluT2 than VGluT1, whereas rostral corneal afferents were more likely to contain VGluT1 than VGluT2. Triple-labeling studies combining CTb, CGRP, and VGluT2 showed that very few corneal afferents contain both CGRP and VGluT2, caudally (1%) and rostrally (2%). These results suggest that most corneal afferents contain a peptide or a VGluT, but rarely both. Our results are consistent with a growing literature suggesting that glutamatergic and peptidergic sensory afferents may be distinct populations.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Oregon Health & Science University, Beaverton, Oregon 97006, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20593358

Citation

Hegarty, Deborah M., et al. "Differential Localization of Vesicular Glutamate Transporters and Peptides in Corneal Afferents to Trigeminal Nucleus Caudalis." The Journal of Comparative Neurology, vol. 518, no. 17, 2010, pp. 3557-69.
Hegarty DM, Tonsfeldt K, Hermes SM, et al. Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis. J Comp Neurol. 2010;518(17):3557-69.
Hegarty, D. M., Tonsfeldt, K., Hermes, S. M., Helfand, H., & Aicher, S. A. (2010). Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis. The Journal of Comparative Neurology, 518(17), 3557-69. https://doi.org/10.1002/cne.22414
Hegarty DM, et al. Differential Localization of Vesicular Glutamate Transporters and Peptides in Corneal Afferents to Trigeminal Nucleus Caudalis. J Comp Neurol. 2010 Sep 1;518(17):3557-69. PubMed PMID: 20593358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis. AU - Hegarty,Deborah M, AU - Tonsfeldt,Karen, AU - Hermes,Sam M, AU - Helfand,Helen, AU - Aicher,Sue A, PY - 2010/7/2/entrez PY - 2010/7/2/pubmed PY - 2010/10/12/medline SP - 3557 EP - 69 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 518 IS - 17 N2 - Trigeminal afferents convey nociceptive information from the corneal surface of the eye to the trigeminal subnucleus caudalis (Vc). Trigeminal afferents, like other nociceptors, are thought to use glutamate and neuropeptides as neurotransmitters. The current studies examined whether corneal afferents contain both neuropeptides and vesicular glutamate transporters. Corneal afferents to the Vc were identified by using cholera toxin B (CTb). Corneal afferents project in two clusters to the rostral and caudal borders of the Vc, regions that contain functionally distinct nociceptive neurons. Thus, corneal afferents projecting to these two regions were examined separately. Dual immunocytochemical studies combined CTb with either calcitonin gene-related peptide (CGRP), substance P (SP), vesicular glutamate transporter 1 (VGluT1), or VGluT2. Corneal afferents were more likely to contain CGRP than SP, and corneal afferents projecting to the rostral region were more likely to contain CGRP than afferents projecting caudally. Overall, corneal afferents were equally likely to contain VGluT1 or VGluT2. Together, 61% of corneal afferents contained either VGluT1 or VGluT2, suggesting that some afferents lack a VGluT. Caudal corneal afferents were more likely to contain VGluT2 than VGluT1, whereas rostral corneal afferents were more likely to contain VGluT1 than VGluT2. Triple-labeling studies combining CTb, CGRP, and VGluT2 showed that very few corneal afferents contain both CGRP and VGluT2, caudally (1%) and rostrally (2%). These results suggest that most corneal afferents contain a peptide or a VGluT, but rarely both. Our results are consistent with a growing literature suggesting that glutamatergic and peptidergic sensory afferents may be distinct populations. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/20593358/Differential_localization_of_vesicular_glutamate_transporters_and_peptides_in_corneal_afferents_to_trigeminal_nucleus_caudalis_ L2 - https://doi.org/10.1002/cne.22414 DB - PRIME DP - Unbound Medicine ER -