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Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents.
BMC Plant Biol. 2010 Jul 01; 10:137.BP

Abstract

BACKGROUND

FAE1 (fatty acid elongase1) is the key gene in the control of erucic acid synthesis in seeds of Brassica species. Due to oil with low erucic acid (LEA) content is essential for human health and not enough LEA resource could be available, thus new LEA genetic resources are being sought for Brassica breeding. EcoTILLING, a powerful genotyping method, can readily be used to identify polymorphisms in Brassica.

RESULTS

Seven B. rapa, nine B. oleracea and 101 B. napus accessions were collected for identification of FAE1 polymorphisms. Three polymorphisms were detected in the two FAE1 paralogues of B. napus using EcoTILLING and were found to be strongly associated with differences in the erucic acid contents of seeds. In genomic FAE1 sequences obtained from seven B. rapa accessions, one SNP in the coding region was deduced to cause loss of gene function. Molecular evolution analysis of FAE1 homologues showed that the relationship between the Brassica A and C genomes is closer than that between the A/C genomes and Arabidopsis genome. Alignment of the coding sequences of these FAE1 homologues indicated that 18 SNPs differed between the A and C genomes and could be used as genome-specific markers in Brassica.

CONCLUSION

This study showed the applicability of EcoTILLING for detecting gene polymorphisms in Brassica. The association between B. napus FAE1 polymorphisms and the erucic acid contents of seeds may provide useful guidance for LEA breeding. The discovery of the LEA resource in B. rapa can be exploited in Brasscia cultivation.

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Authors+Show Affiliations

Wang N
National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
Shi L
No affiliation info available
Tian F
No affiliation info available
Ning H
No affiliation info available
Wu X
No affiliation info available
Long Y
No affiliation info available
Meng J
No affiliation info available

MeSH

AcetyltransferasesBrassicaErucic AcidsFatty Acid ElongasesGenotypeHumansMolecular Sequence DataPhenotypePhylogenyPolymorphism, GeneticPolymorphism, Single NucleotideSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20594317

Citation

Wang, Nian, et al. "Assessment of FAE1 Polymorphisms in Three Brassica Species Using EcoTILLING and Their Association With Differences in Seed Erucic Acid Contents." BMC Plant Biology, vol. 10, 2010, p. 137.
Wang N, Shi L, Tian F, et al. Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents. BMC Plant Biol. 2010;10:137.
Wang, N., Shi, L., Tian, F., Ning, H., Wu, X., Long, Y., & Meng, J. (2010). Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents. BMC Plant Biology, 10, 137. https://doi.org/10.1186/1471-2229-10-137
Wang N, et al. Assessment of FAE1 Polymorphisms in Three Brassica Species Using EcoTILLING and Their Association With Differences in Seed Erucic Acid Contents. BMC Plant Biol. 2010 Jul 1;10:137. PubMed PMID: 20594317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents. AU - Wang,Nian, AU - Shi,Lei, AU - Tian,Fang, AU - Ning,Huicai, AU - Wu,Xiaoming, AU - Long,Yan, AU - Meng,Jinling, Y1 - 2010/07/01/ PY - 2009/07/12/received PY - 2010/07/01/accepted PY - 2010/7/3/entrez PY - 2010/7/3/pubmed PY - 2010/10/7/medline SP - 137 EP - 137 JF - BMC plant biology JO - BMC Plant Biol VL - 10 N2 - BACKGROUND: FAE1 (fatty acid elongase1) is the key gene in the control of erucic acid synthesis in seeds of Brassica species. Due to oil with low erucic acid (LEA) content is essential for human health and not enough LEA resource could be available, thus new LEA genetic resources are being sought for Brassica breeding. EcoTILLING, a powerful genotyping method, can readily be used to identify polymorphisms in Brassica. RESULTS: Seven B. rapa, nine B. oleracea and 101 B. napus accessions were collected for identification of FAE1 polymorphisms. Three polymorphisms were detected in the two FAE1 paralogues of B. napus using EcoTILLING and were found to be strongly associated with differences in the erucic acid contents of seeds. In genomic FAE1 sequences obtained from seven B. rapa accessions, one SNP in the coding region was deduced to cause loss of gene function. Molecular evolution analysis of FAE1 homologues showed that the relationship between the Brassica A and C genomes is closer than that between the A/C genomes and Arabidopsis genome. Alignment of the coding sequences of these FAE1 homologues indicated that 18 SNPs differed between the A and C genomes and could be used as genome-specific markers in Brassica. CONCLUSION: This study showed the applicability of EcoTILLING for detecting gene polymorphisms in Brassica. The association between B. napus FAE1 polymorphisms and the erucic acid contents of seeds may provide useful guidance for LEA breeding. The discovery of the LEA resource in B. rapa can be exploited in Brasscia cultivation. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/20594317/Assessment_of_FAE1_polymorphisms_in_three_Brassica_species_using_EcoTILLING_and_their_association_with_differences_in_seed_erucic_acid_contents_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-137 DB - PRIME DP - Unbound Medicine ER -