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Analysis of nerve and neuropeptide patterns in vacuum-assisted closure-treated diabetic murine wounds.
Plast Reconstr Surg 2010; 126(1):87-96PR

Abstract

BACKGROUND

Reestablishment of the peripheral nervous system occurs in parallel with wound healing. With accelerated wound healing seen with the vacuum-assisted closure device, the authors studied its effects on nerve fiber regeneration, nerve sprouting, and the stimulation of neuropeptides and neurotrophins.

METHODS

A vacuum-assisted closure device was applied to a full-thickness diabetic mouse wound using continuous or cyclical modes and compared with foam dressing or occlusive dressing controls, using 10 mice per group. Nerve fibers, substance P, calcitonin gene-related peptide, and nerve growth factor were analyzed using two-dimensional immunohistochemistry and real-time reverse-transcriptase polymerase chain reaction.

RESULTS

A significant increase in dermal and epidermal nerve fiber densities and in substance P, calcitonin gene-related peptide, and nerve growth factor expression was seen in vacuum-assisted closure-treated wounds. Cyclical treatment mode correlated with the largest increase in granulation tissue production, wound surface microdeformations, and a slightly faster wound closure rate.

CONCLUSIONS

This study suggests that vacuum-assisted closure therapy can modulate nerve fiber and neuropeptide production in the wound. Optimized kinetics of vacuum-assisted closure application may provide an opportunity for clinicians to further improve wound healing in denervated wounds such as pressure sores and diabetic foot ulcerations.

Authors+Show Affiliations

Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass. 02115, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

20595860

Citation

Younan, George, et al. "Analysis of Nerve and Neuropeptide Patterns in Vacuum-assisted Closure-treated Diabetic Murine Wounds." Plastic and Reconstructive Surgery, vol. 126, no. 1, 2010, pp. 87-96.
Younan G, Ogawa R, Ramirez M, et al. Analysis of nerve and neuropeptide patterns in vacuum-assisted closure-treated diabetic murine wounds. Plast Reconstr Surg. 2010;126(1):87-96.
Younan, G., Ogawa, R., Ramirez, M., Helm, D., Dastouri, P., & Orgill, D. P. (2010). Analysis of nerve and neuropeptide patterns in vacuum-assisted closure-treated diabetic murine wounds. Plastic and Reconstructive Surgery, 126(1), pp. 87-96. doi:10.1097/PRS.0b013e3181da86d0.
Younan G, et al. Analysis of Nerve and Neuropeptide Patterns in Vacuum-assisted Closure-treated Diabetic Murine Wounds. Plast Reconstr Surg. 2010;126(1):87-96. PubMed PMID: 20595860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of nerve and neuropeptide patterns in vacuum-assisted closure-treated diabetic murine wounds. AU - Younan,George, AU - Ogawa,Rei, AU - Ramirez,Michelle, AU - Helm,Douglas, AU - Dastouri,Pouya, AU - Orgill,Dennis P, PY - 2010/7/3/entrez PY - 2010/7/3/pubmed PY - 2010/9/3/medline SP - 87 EP - 96 JF - Plastic and reconstructive surgery JO - Plast. Reconstr. Surg. VL - 126 IS - 1 N2 - BACKGROUND: Reestablishment of the peripheral nervous system occurs in parallel with wound healing. With accelerated wound healing seen with the vacuum-assisted closure device, the authors studied its effects on nerve fiber regeneration, nerve sprouting, and the stimulation of neuropeptides and neurotrophins. METHODS: A vacuum-assisted closure device was applied to a full-thickness diabetic mouse wound using continuous or cyclical modes and compared with foam dressing or occlusive dressing controls, using 10 mice per group. Nerve fibers, substance P, calcitonin gene-related peptide, and nerve growth factor were analyzed using two-dimensional immunohistochemistry and real-time reverse-transcriptase polymerase chain reaction. RESULTS: A significant increase in dermal and epidermal nerve fiber densities and in substance P, calcitonin gene-related peptide, and nerve growth factor expression was seen in vacuum-assisted closure-treated wounds. Cyclical treatment mode correlated with the largest increase in granulation tissue production, wound surface microdeformations, and a slightly faster wound closure rate. CONCLUSIONS: This study suggests that vacuum-assisted closure therapy can modulate nerve fiber and neuropeptide production in the wound. Optimized kinetics of vacuum-assisted closure application may provide an opportunity for clinicians to further improve wound healing in denervated wounds such as pressure sores and diabetic foot ulcerations. SN - 1529-4242 UR - https://www.unboundmedicine.com/medline/citation/20595860/Analysis_of_nerve_and_neuropeptide_patterns_in_vacuum_assisted_closure_treated_diabetic_murine_wounds_ L2 - http://Insights.ovid.com/pubmed?pmid=20595860 DB - PRIME DP - Unbound Medicine ER -