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Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751, a recombinant protein of Treponema pallidum.
Sci China Life Sci 2010; 53(2):229-33SC

Abstract

The tissue destruction characteristic of syphilis infection may be caused by inflammation due to Treponema pallidum and the ensuing immune responses to the pathogen. T. pallidum membrane proteins are thought to be potent inducers of inflammation during the early stages of infection. However, the actual membrane proteins that induce inflammatory cytokine production are not known, nor are the molecular mechanisms responsible for triggering and sustaining the inflammatory cascades. In the present study, Tp0751 recombinant protein from T. pallidum was found to induce the production of proinflammatory cytokines, including TNF-alpha, IL-1beta and IL-6, in a THP-1 human monocyte cell line. The signal transduction pathways involved in the production of these cytokines were then further investigated. No inhibition of TNF-a, IL-1beta, or IL-6 production was observed following treatment with the SAPK/JNK specific inhibitor SP600125 or with an ERK inhibitor PD98059. By contrast, anti-TLR2 mAb, anti-CD14 mAb, and the p38 inhibitor SB203580 significantly inhibited the production of all three cytokines. In addition, pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of NF-kappaB, profoundly inhibited the production of these cytokines. Tp0751 treatment strongly activated NF-kappaB, as revealed by Western blotting. However, NF-kappaB translocation was significantly inhibited by treatment with PDTC. These results indicated that TLR2, CD14, MAPKs/p38, and NF-kappaB might be implicated in the inflammatory reaction caused by T. pallidum infection.

Authors+Show Affiliations

Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha, 410078, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20596832

Citation

Liu, ShuangQuan, et al. "Production of Proinflammatory Cytokines in the Human THP-1 Monocyte Cell Line Following Induction By Tp0751, a Recombinant Protein of Treponema Pallidum." Science China. Life Sciences, vol. 53, no. 2, 2010, pp. 229-33.
Liu S, Wang S, Wu Y, et al. Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751, a recombinant protein of Treponema pallidum. Sci China Life Sci. 2010;53(2):229-33.
Liu, S., Wang, S., Wu, Y., Zhao, F., Zeng, T., Zhang, Y., ... Gao, D. (2010). Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751, a recombinant protein of Treponema pallidum. Science China. Life Sciences, 53(2), pp. 229-33. doi:10.1007/s11427-010-0038-z.
Liu S, et al. Production of Proinflammatory Cytokines in the Human THP-1 Monocyte Cell Line Following Induction By Tp0751, a Recombinant Protein of Treponema Pallidum. Sci China Life Sci. 2010;53(2):229-33. PubMed PMID: 20596832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of proinflammatory cytokines in the human THP-1 monocyte cell line following induction by Tp0751, a recombinant protein of Treponema pallidum. AU - Liu,ShuangQuan, AU - Wang,ShiPing, AU - Wu,YiMou, AU - Zhao,FeiJun, AU - Zeng,TieBing, AU - Zhang,YueJun, AU - Zhang,QiuGui, AU - Gao,DongMei, Y1 - 2010/03/07/ PY - 2009/10/09/received PY - 2009/12/17/accepted PY - 2010/7/3/entrez PY - 2010/7/3/pubmed PY - 2010/10/21/medline SP - 229 EP - 33 JF - Science China. Life sciences JO - Sci China Life Sci VL - 53 IS - 2 N2 - The tissue destruction characteristic of syphilis infection may be caused by inflammation due to Treponema pallidum and the ensuing immune responses to the pathogen. T. pallidum membrane proteins are thought to be potent inducers of inflammation during the early stages of infection. However, the actual membrane proteins that induce inflammatory cytokine production are not known, nor are the molecular mechanisms responsible for triggering and sustaining the inflammatory cascades. In the present study, Tp0751 recombinant protein from T. pallidum was found to induce the production of proinflammatory cytokines, including TNF-alpha, IL-1beta and IL-6, in a THP-1 human monocyte cell line. The signal transduction pathways involved in the production of these cytokines were then further investigated. No inhibition of TNF-a, IL-1beta, or IL-6 production was observed following treatment with the SAPK/JNK specific inhibitor SP600125 or with an ERK inhibitor PD98059. By contrast, anti-TLR2 mAb, anti-CD14 mAb, and the p38 inhibitor SB203580 significantly inhibited the production of all three cytokines. In addition, pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of NF-kappaB, profoundly inhibited the production of these cytokines. Tp0751 treatment strongly activated NF-kappaB, as revealed by Western blotting. However, NF-kappaB translocation was significantly inhibited by treatment with PDTC. These results indicated that TLR2, CD14, MAPKs/p38, and NF-kappaB might be implicated in the inflammatory reaction caused by T. pallidum infection. SN - 1869-1889 UR - https://www.unboundmedicine.com/medline/citation/20596832/Production_of_proinflammatory_cytokines_in_the_human_THP_1_monocyte_cell_line_following_induction_by_Tp0751_a_recombinant_protein_of_Treponema_pallidum_ DB - PRIME DP - Unbound Medicine ER -