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High performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry for sensitive determination of bioactive amines in donkey milk.
J Chromatogr A. 2010 Aug 06; 1217(32):5215-24.JC

Abstract

In the present study we report on the optimization and validation of a sensitive high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) method for the determination of 8 bioactive amines (histamine, tyramine, tryptamine, 2-phenylethylamine, cadaverine, putrescine, spermidine and spermine) in donkey milk samples. The method involves donkey milk pre-treatment to remove proteins and pre-column dansylation of the amines. HPLC in reversed phase mode has been used for bioactive amines separation and the operating condition of the APCI-MS system proved to be powerful and very efficient for peak assignment. The separation was accomplished in a short time with an excellent resolution for all the amine peaks. Quantification was carried out by monitoring the characteristic [M+H](+) ion of each amine derivative. The method sensitivity, linearity and repeatability were assayed with satisfactory results. The detection limits of the analysed amines ranged from 0.5 microg L(-1) to 15 microg L(-1); the highest LOD was for spermine. Also remarkably good recovery values were obtained; at the lowest spiking level (1 microg L(-1)) the percent mean recoveries ranged from 77.7 to 109.7. Furthermore, as the investigations relate to a complex matrix as donkey milk, suitable studies on matrix effect were performed. Finally, the developed and validated method was applied to analyse 13 donkey milk samples. Among the identified bioactive amines, putrescine, spermine and spermidine proved to be the main amines in donkey milk. Their concentration levels in the present study were lower than the values determined in mature human, cow and sow milk.

Authors+Show Affiliations

Dipartimento di Scienze degli Alimenti e dell'Ambiente Prof. Guglielmo Stagno d'Alcontres, Facoltà di Scienze MM. FF. NN., Università di Messina, Viale Ferdinando Stagno d'Alcontres, 31-98166 Messina, Italy. llatorre@unime.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20598311

Citation

La Torre, Giovanna Loredana, et al. "High Performance Liquid Chromatography Coupled With Atmospheric Pressure Chemical Ionization Mass Spectrometry for Sensitive Determination of Bioactive Amines in Donkey Milk." Journal of Chromatography. A, vol. 1217, no. 32, 2010, pp. 5215-24.
La Torre GL, Saitta M, Giorgia Potortì A, et al. High performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry for sensitive determination of bioactive amines in donkey milk. J Chromatogr A. 2010;1217(32):5215-24.
La Torre, G. L., Saitta, M., Giorgia Potortì, A., Di Bella, G., & Dugo, G. (2010). High performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry for sensitive determination of bioactive amines in donkey milk. Journal of Chromatography. A, 1217(32), 5215-24. https://doi.org/10.1016/j.chroma.2010.06.022
La Torre GL, et al. High Performance Liquid Chromatography Coupled With Atmospheric Pressure Chemical Ionization Mass Spectrometry for Sensitive Determination of Bioactive Amines in Donkey Milk. J Chromatogr A. 2010 Aug 6;1217(32):5215-24. PubMed PMID: 20598311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High performance liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry for sensitive determination of bioactive amines in donkey milk. AU - La Torre,Giovanna Loredana, AU - Saitta,Marcello, AU - Giorgia Potortì,Angela, AU - Di Bella,Giuseppa, AU - Dugo,Giacomo, Y1 - 2010/06/15/ PY - 2010/02/19/received PY - 2010/06/07/revised PY - 2010/06/08/accepted PY - 2010/7/6/entrez PY - 2010/7/6/pubmed PY - 2010/10/20/medline SP - 5215 EP - 24 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 32 N2 - In the present study we report on the optimization and validation of a sensitive high performance liquid chromatography atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) method for the determination of 8 bioactive amines (histamine, tyramine, tryptamine, 2-phenylethylamine, cadaverine, putrescine, spermidine and spermine) in donkey milk samples. The method involves donkey milk pre-treatment to remove proteins and pre-column dansylation of the amines. HPLC in reversed phase mode has been used for bioactive amines separation and the operating condition of the APCI-MS system proved to be powerful and very efficient for peak assignment. The separation was accomplished in a short time with an excellent resolution for all the amine peaks. Quantification was carried out by monitoring the characteristic [M+H](+) ion of each amine derivative. The method sensitivity, linearity and repeatability were assayed with satisfactory results. The detection limits of the analysed amines ranged from 0.5 microg L(-1) to 15 microg L(-1); the highest LOD was for spermine. Also remarkably good recovery values were obtained; at the lowest spiking level (1 microg L(-1)) the percent mean recoveries ranged from 77.7 to 109.7. Furthermore, as the investigations relate to a complex matrix as donkey milk, suitable studies on matrix effect were performed. Finally, the developed and validated method was applied to analyse 13 donkey milk samples. Among the identified bioactive amines, putrescine, spermine and spermidine proved to be the main amines in donkey milk. Their concentration levels in the present study were lower than the values determined in mature human, cow and sow milk. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20598311/High_performance_liquid_chromatography_coupled_with_atmospheric_pressure_chemical_ionization_mass_spectrometry_for_sensitive_determination_of_bioactive_amines_in_donkey_milk_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00792-2 DB - PRIME DP - Unbound Medicine ER -