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Transient transcription of a putative RNase containing BEN domain encoded in Cotesia plutellae bracovirus induces an immunosuppression of the diamondback moth, Plutella xylostella.
J Invertebr Pathol. 2010 Oct; 105(2):156-63.JI

Abstract

A polydnavirus, Cotesia plutellae bracovirus (CpBV), possesses segmented genome located on chromosome(s) of an endoparasitoid wasp, C. plutellae. An episomal viral segment (CpBV-S3) consists of 11,017 bp and encodes two putative open reading frames (ORFs). ORF301 shows amino acid sequence homologies (28-50%) with RNase T2s of various organisms. It also contains BEN domain in C-terminal region. ORF302 is a hypothetical gene, which is also found in other bracoviruses. Both genes were expressed in larvae of Plutella xylostella parasitized by C. plutellae. Their expressions were detected in all tested tissues including hemocyte, fat body, gut, and epidermis. To analyze effects of these genes on the parasitism, the segment of CpBV-S3 was injected to nonparasitized larvae of P. xylostella, in which the two genes were expressed at least for 4 days post-injection. The larvae injected with CpBV-S3 exhibited significant immunosuppression, such as reduction in total hemocyte population and impairment in nodule formation behavior of hemocytes in response to bacterial challenge. Each gene expression in the treated larvae was inhibited by co-injecting respective double strand RNA (dsRNA) specific to each ORF. Injection of dsRNA of ORF301 could rescue the immunosuppression of the viral segment-treated larvae, while dsRNA specific to ORF302 did not. These results suggest that a putative RNase fused with a BEN domain encoded in CpBV-S3 plays a parasitic role in inducing host immunosuppression in the parasitism.

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Authors+Show Affiliations

Park B
Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.
Kim Y
No affiliation info available

MeSH

Amino Acid SequenceAnimalsGene Expression Regulation, ViralGenes, InsectHemocytesHost-Pathogen InteractionsImmune ToleranceInsect ProteinsMolecular Sequence DataMothsOpen Reading FramesPolydnaviridaeRNA, ViralRibonucleasesSequence HomologyTranscription, GeneticViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20600089

Citation

Park, Bokri, and Yonggyun Kim. "Transient Transcription of a Putative RNase Containing BEN Domain Encoded in Cotesia Plutellae Bracovirus Induces an Immunosuppression of the Diamondback Moth, Plutella Xylostella." Journal of Invertebrate Pathology, vol. 105, no. 2, 2010, pp. 156-63.
Park B, Kim Y. Transient transcription of a putative RNase containing BEN domain encoded in Cotesia plutellae bracovirus induces an immunosuppression of the diamondback moth, Plutella xylostella. J Invertebr Pathol. 2010;105(2):156-63.
Park, B., & Kim, Y. (2010). Transient transcription of a putative RNase containing BEN domain encoded in Cotesia plutellae bracovirus induces an immunosuppression of the diamondback moth, Plutella xylostella. Journal of Invertebrate Pathology, 105(2), 156-63. https://doi.org/10.1016/j.jip.2010.06.003
Park B, Kim Y. Transient Transcription of a Putative RNase Containing BEN Domain Encoded in Cotesia Plutellae Bracovirus Induces an Immunosuppression of the Diamondback Moth, Plutella Xylostella. J Invertebr Pathol. 2010;105(2):156-63. PubMed PMID: 20600089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transient transcription of a putative RNase containing BEN domain encoded in Cotesia plutellae bracovirus induces an immunosuppression of the diamondback moth, Plutella xylostella. AU - Park,Bokri, AU - Kim,Yonggyun, Y1 - 2010/06/17/ PY - 2010/01/27/received PY - 2010/06/02/revised PY - 2010/06/03/accepted PY - 2010/7/6/entrez PY - 2010/7/6/pubmed PY - 2011/1/20/medline SP - 156 EP - 63 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 105 IS - 2 N2 - A polydnavirus, Cotesia plutellae bracovirus (CpBV), possesses segmented genome located on chromosome(s) of an endoparasitoid wasp, C. plutellae. An episomal viral segment (CpBV-S3) consists of 11,017 bp and encodes two putative open reading frames (ORFs). ORF301 shows amino acid sequence homologies (28-50%) with RNase T2s of various organisms. It also contains BEN domain in C-terminal region. ORF302 is a hypothetical gene, which is also found in other bracoviruses. Both genes were expressed in larvae of Plutella xylostella parasitized by C. plutellae. Their expressions were detected in all tested tissues including hemocyte, fat body, gut, and epidermis. To analyze effects of these genes on the parasitism, the segment of CpBV-S3 was injected to nonparasitized larvae of P. xylostella, in which the two genes were expressed at least for 4 days post-injection. The larvae injected with CpBV-S3 exhibited significant immunosuppression, such as reduction in total hemocyte population and impairment in nodule formation behavior of hemocytes in response to bacterial challenge. Each gene expression in the treated larvae was inhibited by co-injecting respective double strand RNA (dsRNA) specific to each ORF. Injection of dsRNA of ORF301 could rescue the immunosuppression of the viral segment-treated larvae, while dsRNA specific to ORF302 did not. These results suggest that a putative RNase fused with a BEN domain encoded in CpBV-S3 plays a parasitic role in inducing host immunosuppression in the parasitism. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/20600089/Transient_transcription_of_a_putative_RNase_containing_BEN_domain_encoded_in_Cotesia_plutellae_bracovirus_induces_an_immunosuppression_of_the_diamondback_moth_Plutella_xylostella_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(10)00141-2 DB - PRIME DP - Unbound Medicine ER -