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Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry.
Biochim Biophys Acta. 2010 Sep; 1803(9):1083-93.BB

Abstract

Different studies have reported that proteins involved in Ca(2+) entry are localized in discrete plasma membrane domains known as lipid rafts, which have been suggested to support store-operated Ca(2+) entry by facilitating STIM1 clustering in endoplasmic reticulum-plasma membrane junctions as well as the interaction of STIM1 with TRPC1. Here we report that treatment of HEK293 cells with thapsigargin (TG) results in the activation of Ca(2+) entry with two components, an early, La(3+)-sensitive, component and a late component that shows both La(3+)-sensitive and -insensitive constituents. Preincubation with methyl-beta-cyclodextrin (MbetaCD) prevented TG-induced activation of Ca(2+) entry but, in contrast, enhanced this process after its activation. Addition of MbetaCD after store depletion did not modify the La(3+)-sensitive store-operated divalent cation entry but increased La(3+)-insensitive non-capacitative Ca(2+) entry. Cell stimulation with TG results in a transient increase in Orai1 co-immunoprecipitation with STIM1, TRPC1 and TRPC6. TG-induced association of these proteins was significantly attenuated by preincubation for 30 min with MbetaCD, without altering surface expression of Orai1 or TRPCs. In contrast, the association of Orai1 with STIM1 or TRPC1 was unaffected when MbetaCD was added after store depletion with TG. Addition of MbetaCD to TG-treated cells promoted dissociation between Orai1 and TRPC6, as well as non-capacitative Ca(2+) entry. TRPC6 expression silencing indicates that MbetaCD-enhanced non-capacitative Ca(2+) entry was mediated by TRPC6. In conclusion, lipid raft domains are necessary for the activation but not the maintenance of SOCE probably due to the support of the formation of Ca(2+) signalling complexes involving Orai1, TRPCs and STIM1.

Authors+Show Affiliations

Department of Physiology (Cell Physiology Research Group), University of Extremadura, 10071 Caceres, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20600358

Citation

Galan, Carmen, et al. "Lipid Rafts Modulate the Activation but Not the Maintenance of Store-operated Ca(2+) Entry." Biochimica Et Biophysica Acta, vol. 1803, no. 9, 2010, pp. 1083-93.
Galan C, Woodard GE, Dionisio N, et al. Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry. Biochim Biophys Acta. 2010;1803(9):1083-93.
Galan, C., Woodard, G. E., Dionisio, N., Salido, G. M., & Rosado, J. A. (2010). Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry. Biochimica Et Biophysica Acta, 1803(9), 1083-93. https://doi.org/10.1016/j.bbamcr.2010.06.006
Galan C, et al. Lipid Rafts Modulate the Activation but Not the Maintenance of Store-operated Ca(2+) Entry. Biochim Biophys Acta. 2010;1803(9):1083-93. PubMed PMID: 20600358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid rafts modulate the activation but not the maintenance of store-operated Ca(2+) entry. AU - Galan,Carmen, AU - Woodard,Geoffrey E, AU - Dionisio,Natalia, AU - Salido,Gines M, AU - Rosado,Juan A, Y1 - 2010/06/22/ PY - 2010/04/05/received PY - 2010/06/02/revised PY - 2010/06/11/accepted PY - 2010/7/6/entrez PY - 2010/7/6/pubmed PY - 2010/10/28/medline SP - 1083 EP - 93 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1803 IS - 9 N2 - Different studies have reported that proteins involved in Ca(2+) entry are localized in discrete plasma membrane domains known as lipid rafts, which have been suggested to support store-operated Ca(2+) entry by facilitating STIM1 clustering in endoplasmic reticulum-plasma membrane junctions as well as the interaction of STIM1 with TRPC1. Here we report that treatment of HEK293 cells with thapsigargin (TG) results in the activation of Ca(2+) entry with two components, an early, La(3+)-sensitive, component and a late component that shows both La(3+)-sensitive and -insensitive constituents. Preincubation with methyl-beta-cyclodextrin (MbetaCD) prevented TG-induced activation of Ca(2+) entry but, in contrast, enhanced this process after its activation. Addition of MbetaCD after store depletion did not modify the La(3+)-sensitive store-operated divalent cation entry but increased La(3+)-insensitive non-capacitative Ca(2+) entry. Cell stimulation with TG results in a transient increase in Orai1 co-immunoprecipitation with STIM1, TRPC1 and TRPC6. TG-induced association of these proteins was significantly attenuated by preincubation for 30 min with MbetaCD, without altering surface expression of Orai1 or TRPCs. In contrast, the association of Orai1 with STIM1 or TRPC1 was unaffected when MbetaCD was added after store depletion with TG. Addition of MbetaCD to TG-treated cells promoted dissociation between Orai1 and TRPC6, as well as non-capacitative Ca(2+) entry. TRPC6 expression silencing indicates that MbetaCD-enhanced non-capacitative Ca(2+) entry was mediated by TRPC6. In conclusion, lipid raft domains are necessary for the activation but not the maintenance of SOCE probably due to the support of the formation of Ca(2+) signalling complexes involving Orai1, TRPCs and STIM1. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/20600358/Lipid_rafts_modulate_the_activation_but_not_the_maintenance_of_store_operated_Ca_2+__entry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-4889(10)00176-X DB - PRIME DP - Unbound Medicine ER -