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N-stearoyltyrosine protects primary neurons from Aβ-induced apoptosis through modulating mitogen-activated protein kinase activity.
Neuroscience. 2010 Sep 15; 169(4):1840-7.N

Abstract

N-stearoyltyrosine (NsTyr), an anandamide (AEA) analogue is similar to AEA not only structurally but also in terms of biological activity. Since A beta-induced neuronal injury triggers the activation of mitogen-activated protein kinase (MAPK) pathways and the induction or activation of pro- and anti-apoptotic proteins, in the present study we aimed to assess the protective effect of NsTyr against A beta induced neuronal apoptosis. Cell viability and neuronal injury were respectively measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) assay. Hoechst staining and flow cytometric assessment were used to evaluate cell apoptosis. The anti-apoptotic mechanism involved MAPK phosphorylation and Bcl-2/Bax expression was investigated. The best neuroprotective effect on A beta 25-35-induced neuronal apoptosis was observed in the presence of NsTyr (1 microM). NsTyr exerted anti-apoptotic effect at least partly via activating p-ERK-Bcl-2 but suppressing p-p38-Bax pathways. Moreover a dynamic balance between p-ERK and p-p38 MAPK pathways in NsTyr-induced neuronal protection suggested an interaction between them. Our results indicated the neuroprotective effect of NsTyr on A beta 25-35-induced neuronal injury was at least partly due to anti-apoptosis and raised the possibility that NsTyr might reduce neurodegenerative disorders.

Authors+Show Affiliations

Department of Pharmacy, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20600674

Citation

Yang, Z H., et al. "N-stearoyltyrosine Protects Primary Neurons From Aβ-induced Apoptosis Through Modulating Mitogen-activated Protein Kinase Activity." Neuroscience, vol. 169, no. 4, 2010, pp. 1840-7.
Yang ZH, Sun K, Suo WH, et al. N-stearoyltyrosine protects primary neurons from Aβ-induced apoptosis through modulating mitogen-activated protein kinase activity. Neuroscience. 2010;169(4):1840-7.
Yang, Z. H., Sun, K., Suo, W. H., Yao, L. Y., Fu, Q., Cui, Y. Y., Fu, G. H., Chen, H. Z., & Lu, Y. (2010). N-stearoyltyrosine protects primary neurons from Aβ-induced apoptosis through modulating mitogen-activated protein kinase activity. Neuroscience, 169(4), 1840-7. https://doi.org/10.1016/j.neuroscience.2010.05.069
Yang ZH, et al. N-stearoyltyrosine Protects Primary Neurons From Aβ-induced Apoptosis Through Modulating Mitogen-activated Protein Kinase Activity. Neuroscience. 2010 Sep 15;169(4):1840-7. PubMed PMID: 20600674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-stearoyltyrosine protects primary neurons from Aβ-induced apoptosis through modulating mitogen-activated protein kinase activity. AU - Yang,Z H, AU - Sun,K, AU - Suo,W H, AU - Yao,L Y, AU - Fu,Q, AU - Cui,Y Y, AU - Fu,G H, AU - Chen,H Z, AU - Lu,Y, Y1 - 2010/06/19/ PY - 2010/02/10/received PY - 2010/05/26/revised PY - 2010/05/26/accepted PY - 2010/7/6/entrez PY - 2010/7/6/pubmed PY - 2011/4/19/medline SP - 1840 EP - 7 JF - Neuroscience JO - Neuroscience VL - 169 IS - 4 N2 - N-stearoyltyrosine (NsTyr), an anandamide (AEA) analogue is similar to AEA not only structurally but also in terms of biological activity. Since A beta-induced neuronal injury triggers the activation of mitogen-activated protein kinase (MAPK) pathways and the induction or activation of pro- and anti-apoptotic proteins, in the present study we aimed to assess the protective effect of NsTyr against A beta induced neuronal apoptosis. Cell viability and neuronal injury were respectively measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) assay. Hoechst staining and flow cytometric assessment were used to evaluate cell apoptosis. The anti-apoptotic mechanism involved MAPK phosphorylation and Bcl-2/Bax expression was investigated. The best neuroprotective effect on A beta 25-35-induced neuronal apoptosis was observed in the presence of NsTyr (1 microM). NsTyr exerted anti-apoptotic effect at least partly via activating p-ERK-Bcl-2 but suppressing p-p38-Bax pathways. Moreover a dynamic balance between p-ERK and p-p38 MAPK pathways in NsTyr-induced neuronal protection suggested an interaction between them. Our results indicated the neuroprotective effect of NsTyr on A beta 25-35-induced neuronal injury was at least partly due to anti-apoptosis and raised the possibility that NsTyr might reduce neurodegenerative disorders. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/20600674/N_stearoyltyrosine_protects_primary_neurons_from_Aβ_induced_apoptosis_through_modulating_mitogen_activated_protein_kinase_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(10)00809-2 DB - PRIME DP - Unbound Medicine ER -