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Surface plasmon resonance immunosensor for bacteria detection.
Talanta. 2010 Jul 15; 82(2):810-4.T

Abstract

This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized gold nanoparticles. For the first biosensor, the gold substrate was functionalized with acid-thiol using the self-assembled monolayer technique, while the second one was functionalized with gold nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) and non-specific (Lactobacillus) bacteria detection. Detection limit with a good reproducibility of 10(4) and 10(3) cfu mL(-1) of E. coli bacteria has been obtained for the first biosensor and for the second one respectively. A refractive index variation below 5x10(-3) due to bacteria adsorption is able to be detected. The refractive index of the multilayer structure and of the E. coli bacteria layer was estimated with a modeling software.

Authors+Show Affiliations

Nanotechnology Laboratory, INSAT, Centre Urbain Nord, Charguia, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20602974

Citation

Baccar, H, et al. "Surface Plasmon Resonance Immunosensor for Bacteria Detection." Talanta, vol. 82, no. 2, 2010, pp. 810-4.
Baccar H, Mejri MB, Hafaiedh I, et al. Surface plasmon resonance immunosensor for bacteria detection. Talanta. 2010;82(2):810-4.
Baccar, H., Mejri, M. B., Hafaiedh, I., Ktari, T., Aouni, M., & Abdelghani, A. (2010). Surface plasmon resonance immunosensor for bacteria detection. Talanta, 82(2), 810-4. https://doi.org/10.1016/j.talanta.2010.05.060
Baccar H, et al. Surface Plasmon Resonance Immunosensor for Bacteria Detection. Talanta. 2010 Jul 15;82(2):810-4. PubMed PMID: 20602974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance immunosensor for bacteria detection. AU - Baccar,H, AU - Mejri,M B, AU - Hafaiedh,I, AU - Ktari,T, AU - Aouni,M, AU - Abdelghani,A, Y1 - 2010/06/04/ PY - 2010/03/05/received PY - 2010/05/28/revised PY - 2010/05/28/accepted PY - 2010/7/7/entrez PY - 2010/7/7/pubmed PY - 2010/11/17/medline SP - 810 EP - 4 JF - Talanta JO - Talanta VL - 82 IS - 2 N2 - This work describes an approach for the development of two bacteria biosensors based on surface plasmon resonance (SPR) technique. The first biosensor was based on functionalized gold substrate and the second one on immobilized gold nanoparticles. For the first biosensor, the gold substrate was functionalized with acid-thiol using the self-assembled monolayer technique, while the second one was functionalized with gold nanoparticles immobilized on modified gold substrate. A polyclonal anti-Escherichia coli antibody was immobilized for specific (E. coli) and non-specific (Lactobacillus) bacteria detection. Detection limit with a good reproducibility of 10(4) and 10(3) cfu mL(-1) of E. coli bacteria has been obtained for the first biosensor and for the second one respectively. A refractive index variation below 5x10(-3) due to bacteria adsorption is able to be detected. The refractive index of the multilayer structure and of the E. coli bacteria layer was estimated with a modeling software. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20602974/Surface_plasmon_resonance_immunosensor_for_bacteria_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00414-5 DB - PRIME DP - Unbound Medicine ER -