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Multi-wavelength light emitting diode array as an excitation source for light emitting diode-induced fluorescence detection in capillary electrophoresis.
Electrophoresis. 2010 Aug; 31(15):2589-95.E

Abstract

A multi-wavelength LED array was used as an excitation source for in-column fiber-optic LED-induced fluorescence for CE. The light source consisted of a multi-wavelength LED array consisting of three different LEDs (430, 450 and 480 nm), a focusing lens and a gradient index lens group. The LED beam was collimated and reshaped with the gradient index lens group for coupling the LED light source into a single-mode optical fiber. In addition, the luminance and stability of the LED light source was improved by powering the LED under constant current at enhanced voltages. The benefits of this system were demonstrated by the simultaneous determination of FITC-labeled L-asparagine (Ex/Em 488/520 nm), 4-fluoro-7-nitro-2,1,3-benzoxadiazole-labeled epinephrine (Ex/Em 468/530 nm) and 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde-labeled L-leucine (Ex/Em 440/530 nm). Detection limits of L-asparagine, epinephrine and L-leucine were estimated to be 0.8 x 10(-9), 12.0 x 10(-8) and 4.0 x 10(-8) M (S/N=3), respectively. The RSDs (n=6) for migration time and peak area were better than 0.71 and 0.92%, respectively. The performance of the developed multi-wavelength LED excitation source was compared to the use of a single-wavelength LED and found to provide superior sensitivity for the three fluorophores used in this study.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Huo F
College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu, P. R. China.
Yuan H
No affiliation info available
Breadmore MC
No affiliation info available
Xiao D
No affiliation info available

MeSH

AsparagineElectrophoresis, CapillaryEpinephrineEquipment DesignFiber Optic TechnologyFluorescenceFluorescent DyesLeucineLimit of Detection

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

20603826

Citation

Huo, Feng, et al. "Multi-wavelength Light Emitting Diode Array as an Excitation Source for Light Emitting Diode-induced Fluorescence Detection in Capillary Electrophoresis." Electrophoresis, vol. 31, no. 15, 2010, pp. 2589-95.
Huo F, Yuan H, Breadmore MC, et al. Multi-wavelength light emitting diode array as an excitation source for light emitting diode-induced fluorescence detection in capillary electrophoresis. Electrophoresis. 2010;31(15):2589-95.
Huo, F., Yuan, H., Breadmore, M. C., & Xiao, D. (2010). Multi-wavelength light emitting diode array as an excitation source for light emitting diode-induced fluorescence detection in capillary electrophoresis. Electrophoresis, 31(15), 2589-95. https://doi.org/10.1002/elps.201000071
Huo F, et al. Multi-wavelength Light Emitting Diode Array as an Excitation Source for Light Emitting Diode-induced Fluorescence Detection in Capillary Electrophoresis. Electrophoresis. 2010;31(15):2589-95. PubMed PMID: 20603826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multi-wavelength light emitting diode array as an excitation source for light emitting diode-induced fluorescence detection in capillary electrophoresis. AU - Huo,Feng, AU - Yuan,Hongyan, AU - Breadmore,Michael C, AU - Xiao,Dan, PY - 2010/7/7/entrez PY - 2010/7/7/pubmed PY - 2010/12/14/medline SP - 2589 EP - 95 JF - Electrophoresis JO - Electrophoresis VL - 31 IS - 15 N2 - A multi-wavelength LED array was used as an excitation source for in-column fiber-optic LED-induced fluorescence for CE. The light source consisted of a multi-wavelength LED array consisting of three different LEDs (430, 450 and 480 nm), a focusing lens and a gradient index lens group. The LED beam was collimated and reshaped with the gradient index lens group for coupling the LED light source into a single-mode optical fiber. In addition, the luminance and stability of the LED light source was improved by powering the LED under constant current at enhanced voltages. The benefits of this system were demonstrated by the simultaneous determination of FITC-labeled L-asparagine (Ex/Em 488/520 nm), 4-fluoro-7-nitro-2,1,3-benzoxadiazole-labeled epinephrine (Ex/Em 468/530 nm) and 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde-labeled L-leucine (Ex/Em 440/530 nm). Detection limits of L-asparagine, epinephrine and L-leucine were estimated to be 0.8 x 10(-9), 12.0 x 10(-8) and 4.0 x 10(-8) M (S/N=3), respectively. The RSDs (n=6) for migration time and peak area were better than 0.71 and 0.92%, respectively. The performance of the developed multi-wavelength LED excitation source was compared to the use of a single-wavelength LED and found to provide superior sensitivity for the three fluorophores used in this study. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/20603826/Multi_wavelength_light_emitting_diode_array_as_an_excitation_source_for_light_emitting_diode_induced_fluorescence_detection_in_capillary_electrophoresis_ L2 - https://doi.org/10.1002/elps.201000071 DB - PRIME DP - Unbound Medicine ER -