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Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles.
Biosens Bioelectron. 2010 Oct 15; 26(2):815-21.BB

Abstract

The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37°C for 6h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL).

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Authors+Show Affiliations

Sener G
Hacettepe University, Department of Chemistry, Ankara, Turkey.
Ozgur E
No affiliation info available
Yılmaz E
No affiliation info available
Uzun L
No affiliation info available
Say R
No affiliation info available
Denizli A
No affiliation info available

MeSH

Biosensing TechniquesEquipment DesignEquipment Failure AnalysisMicro-Electrical-Mechanical SystemsMuramidaseNanoparticlesNanotechnologySurface Properties

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20605089

Citation

Sener, Gulsu, et al. "Quartz Crystal Microbalance Based Nanosensor for Lysozyme Detection With Lysozyme Imprinted Nanoparticles." Biosensors & Bioelectronics, vol. 26, no. 2, 2010, pp. 815-21.
Sener G, Ozgur E, Yılmaz E, et al. Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles. Biosens Bioelectron. 2010;26(2):815-21.
Sener, G., Ozgur, E., Yılmaz, E., Uzun, L., Say, R., & Denizli, A. (2010). Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles. Biosensors & Bioelectronics, 26(2), 815-21. https://doi.org/10.1016/j.bios.2010.06.003
Sener G, et al. Quartz Crystal Microbalance Based Nanosensor for Lysozyme Detection With Lysozyme Imprinted Nanoparticles. Biosens Bioelectron. 2010 Oct 15;26(2):815-21. PubMed PMID: 20605089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles. AU - Sener,Gulsu, AU - Ozgur,Erdogan, AU - Yılmaz,Erkut, AU - Uzun,Lokman, AU - Say,Ridvan, AU - Denizli,Adil, Y1 - 2010/06/11/ PY - 2010/02/17/received PY - 2010/06/01/revised PY - 2010/06/04/accepted PY - 2010/7/8/entrez PY - 2010/7/8/pubmed PY - 2011/2/8/medline SP - 815 EP - 21 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 2 N2 - The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37°C for 6h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20605089/Quartz_crystal_microbalance_based_nanosensor_for_lysozyme_detection_with_lysozyme_imprinted_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00304-0 DB - PRIME DP - Unbound Medicine ER -