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Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE–HILIC–MS/MS.
J Chromatogr A. 2010 Jul 09; 1217(28):4688-95.JC

Abstract

A rapid, precise and fully-automated method for analysis of folate (vitamin B9) and its catabolites (viz. p-aminobenzoylglutamate and its acetamide derivative) in biofluids is here presented. The method is based on on-line hyphenation of solid-phase extraction (SPE) by a Prospekt 2 system with hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC–MS/MS). The method was analytically characterized by estimation of repeatability (RSD, n = 5, between 0.5 and 4.1%), accuracy (between 96 and 105%), and sensitivity (limits of quantificantion between 0.3 and 8.3 ng/mL (1.1 and 18.8 pmol/mL) or 0.03 and 0.83 ng (0.11 and 1.88 pmol)). The proposed method is suited for routine analysis of folate catabolites as biomarkers to monitor deficiency of vitamin B9.

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Authors+Show Affiliations

Álvarez-Sánchez B
Department of Analytical Chemistry, Campus of Rabanales, University of Córdoba, Spain.
Priego-Capote F
No affiliation info available
Mata-Granados JM
No affiliation info available
Luque de Castro MD
No affiliation info available

MeSH

AutomationChromatography, LiquidFolic AcidHumansHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsLinear ModelsMilk, HumanReproducibility of ResultsSensitivity and SpecificitySolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20605576

Citation

Álvarez-Sánchez, B, et al. "Automated Determination of Folate Catabolites in Human Biofluids (urine, Breast Milk and Serum) By On-line SPE–HILIC–MS/MS." Journal of Chromatography. A, vol. 1217, no. 28, 2010, pp. 4688-95.
Álvarez-Sánchez B, Priego-Capote F, Mata-Granados JM, et al. Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE–HILIC–MS/MS. J Chromatogr A. 2010;1217(28):4688-95.
Álvarez-Sánchez, B., Priego-Capote, F., Mata-Granados, J. M., & Luque de Castro, M. D. (2010). Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE–HILIC–MS/MS. Journal of Chromatography. A, 1217(28), 4688-95. https://doi.org/10.1016/j.chroma.2010.05.010
Álvarez-Sánchez B, et al. Automated Determination of Folate Catabolites in Human Biofluids (urine, Breast Milk and Serum) By On-line SPE–HILIC–MS/MS. J Chromatogr A. 2010 Jul 9;1217(28):4688-95. PubMed PMID: 20605576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Automated determination of folate catabolites in human biofluids (urine, breast milk and serum) by on-line SPE–HILIC–MS/MS. AU - Álvarez-Sánchez,B, AU - Priego-Capote,F, AU - Mata-Granados,J M, AU - Luque de Castro,M D, PY - 2009/12/21/received PY - 2010/05/03/revised PY - 2010/05/04/accepted PY - 2010/7/8/entrez PY - 2010/7/8/pubmed PY - 2011/6/22/medline SP - 4688 EP - 95 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 28 N2 - A rapid, precise and fully-automated method for analysis of folate (vitamin B9) and its catabolites (viz. p-aminobenzoylglutamate and its acetamide derivative) in biofluids is here presented. The method is based on on-line hyphenation of solid-phase extraction (SPE) by a Prospekt 2 system with hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC–MS/MS). The method was analytically characterized by estimation of repeatability (RSD, n = 5, between 0.5 and 4.1%), accuracy (between 96 and 105%), and sensitivity (limits of quantificantion between 0.3 and 8.3 ng/mL (1.1 and 18.8 pmol/mL) or 0.03 and 0.83 ng (0.11 and 1.88 pmol)). The proposed method is suited for routine analysis of folate catabolites as biomarkers to monitor deficiency of vitamin B9. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20605576/Automated_determination_of_folate_catabolites_in_human_biofluids__urine_breast_milk_and_serum__by_on_line_SPE–HILIC–MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00636-9 DB - PRIME DP - Unbound Medicine ER -