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New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay.
J Agric Food Chem. 2010 Aug 11; 58(15):8477-80.JA

Abstract

Several analytical approaches are available for investigating the antioxidant power for antioxidants, and they are based on a variety of chemical principles, such as oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing/antioxidant power (FRAP). This paper reports a new rapid method for investigating antioxidant power on the basis of the electron-donating ability. This method is called chemiluminescence analysis of antioxidant power (CAAP). The electrons donated from antioxidants are capable of inducing chemiluminescence in the presence of lucigenin and a base. Thus, the intensity of chemiluminescence induced by antioxidants is proportional to their electron-donating ability (antioxidant power). It was found that the correlation between CAAP and FRAP was positive (r = 0.959) and statistically significant (p < 0.05). In addition to the FRAP assay, the rapid CAAP assay is convenient for investigating the antioxidant power of herbal extracts.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chen TS
Graduate Institute of Basic Medical Science, China Medical University; Department of Health and Nutrition Biotechnology, Asia University, Taichung 404, Taiwan.
Liou SY
No affiliation info available
Wu HC
No affiliation info available
Tsai FJ
No affiliation info available
Tsai CH
No affiliation info available
Huang CY
No affiliation info available
Chang YL
No affiliation info available

MeSH

AntioxidantsCamelliaChemistry Techniques, AnalyticalChromansFerric CompoundsOxidation-ReductionPanaxPlant ExtractsRhodiolaVitis

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20608750

Citation

Chen, Tung-Sheng, et al. "New Analytical Method for Investigating the Antioxidant Power of Food Extracts On the Basis of Their Electron-donating Ability: Comparison to the Ferric Reducing/antioxidant Power (FRAP) Assay." Journal of Agricultural and Food Chemistry, vol. 58, no. 15, 2010, pp. 8477-80.
Chen TS, Liou SY, Wu HC, et al. New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay. J Agric Food Chem. 2010;58(15):8477-80.
Chen, T. S., Liou, S. Y., Wu, H. C., Tsai, F. J., Tsai, C. H., Huang, C. Y., & Chang, Y. L. (2010). New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay. Journal of Agricultural and Food Chemistry, 58(15), 8477-80. https://doi.org/10.1021/jf9044292
Chen TS, et al. New Analytical Method for Investigating the Antioxidant Power of Food Extracts On the Basis of Their Electron-donating Ability: Comparison to the Ferric Reducing/antioxidant Power (FRAP) Assay. J Agric Food Chem. 2010 Aug 11;58(15):8477-80. PubMed PMID: 20608750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay. AU - Chen,Tung-Sheng, AU - Liou,Show-Yih, AU - Wu,Hsi-Chin, AU - Tsai,Fuu-Jen, AU - Tsai,Chang-Hai, AU - Huang,Chih-Yang, AU - Chang,Yen-Lin, PY - 2010/7/9/entrez PY - 2010/7/9/pubmed PY - 2010/12/14/medline SP - 8477 EP - 80 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 15 N2 - Several analytical approaches are available for investigating the antioxidant power for antioxidants, and they are based on a variety of chemical principles, such as oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing/antioxidant power (FRAP). This paper reports a new rapid method for investigating antioxidant power on the basis of the electron-donating ability. This method is called chemiluminescence analysis of antioxidant power (CAAP). The electrons donated from antioxidants are capable of inducing chemiluminescence in the presence of lucigenin and a base. Thus, the intensity of chemiluminescence induced by antioxidants is proportional to their electron-donating ability (antioxidant power). It was found that the correlation between CAAP and FRAP was positive (r = 0.959) and statistically significant (p < 0.05). In addition to the FRAP assay, the rapid CAAP assay is convenient for investigating the antioxidant power of herbal extracts. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20608750/New_analytical_method_for_investigating_the_antioxidant_power_of_food_extracts_on_the_basis_of_their_electron_donating_ability:_comparison_to_the_ferric_reducing/antioxidant_power__FRAP__assay_ L2 - https://doi.org/10.1021/jf9044292 DB - PRIME DP - Unbound Medicine ER -