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Engineering bone formation from human dental pulp- and periodontal ligament-derived cells.
Ann Biomed Eng. 2011 Jan; 39(1):26-34.AB

Abstract

A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue in vivo from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and in vitro calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of osteocalcin, osteopontin, and dentin matrix protein 1 mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation in vivo when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility.

Authors+Show Affiliations

Department of Regenerative Oral Surgery, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20614244

Citation

Ikeda, Hideyoshi, et al. "Engineering Bone Formation From Human Dental Pulp- and Periodontal Ligament-derived Cells." Annals of Biomedical Engineering, vol. 39, no. 1, 2011, pp. 26-34.
Ikeda H, Sumita Y, Ikeda M, et al. Engineering bone formation from human dental pulp- and periodontal ligament-derived cells. Ann Biomed Eng. 2011;39(1):26-34.
Ikeda, H., Sumita, Y., Ikeda, M., Ikeda, H., Okumura, T., Sakai, E., Nishimura, M., & Asahina, I. (2011). Engineering bone formation from human dental pulp- and periodontal ligament-derived cells. Annals of Biomedical Engineering, 39(1), 26-34. https://doi.org/10.1007/s10439-010-0115-2
Ikeda H, et al. Engineering Bone Formation From Human Dental Pulp- and Periodontal Ligament-derived Cells. Ann Biomed Eng. 2011;39(1):26-34. PubMed PMID: 20614244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineering bone formation from human dental pulp- and periodontal ligament-derived cells. AU - Ikeda,Hideyoshi, AU - Sumita,Yoshinori, AU - Ikeda,Mihoko, AU - Ikeda,Hisazumi, AU - Okumura,Teruhito, AU - Sakai,Eiko, AU - Nishimura,Masahiro, AU - Asahina,Izumi, Y1 - 2010/07/08/ PY - 2010/04/26/received PY - 2010/06/22/accepted PY - 2010/7/9/entrez PY - 2010/7/9/pubmed PY - 2011/4/13/medline SP - 26 EP - 34 JF - Annals of biomedical engineering JO - Ann Biomed Eng VL - 39 IS - 1 N2 - A robust method for inducing bone formation from cultured dental mesenchymal cells has not been established. In this study, a method for generating bone tissue in vivo from cultured human dental pulp- and periodontal ligament-derived cells (DPCs and PDLCs, respectively) was designed using exogenous bone morphogenetic protein 2 (BMP2). DPCs and PDLCs showed enhanced alkaline phosphatase (ALP) activity and calcified nodule formation in medium containing dexamethasone, β-glycerophosphate, and ascorbic acid (osteogenic medium). However, the addition of recombinant human bone morphogenetic protein 2 (rhBMP2) to osteogenic medium remarkably increased ALP activity and in vitro calcification above the increases observed with osteogenic medium alone. rhBMP2 also significantly upregulated the expression of osteocalcin, osteopontin, and dentin matrix protein 1 mRNA in both cell types cultured in osteogenic medium. Finally, we detected prominent bone-like tissue formation in vivo when cells had been exposed to rhBMP2 in osteogenic medium. In contrast, treatments with osteogenic medium or rhBMP2 alone could not induce abundant mineralized tissue formation. We propose here that treatment with rhBMP2 in osteogenic medium can make dental mesenchymal tissues a highly useful source of cells for bone tissue engineering. In addition, both DPCs and PDLCs showed similar and remarkable osteo-inducibility. SN - 1573-9686 UR - https://www.unboundmedicine.com/medline/citation/20614244/Engineering_bone_formation_from_human_dental_pulp__and_periodontal_ligament_derived_cells_ L2 - https://doi.org/10.1007/s10439-010-0115-2 DB - PRIME DP - Unbound Medicine ER -